Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2991 and Pf6N2E2_2992 are separated by 46 nucleotides Pf6N2E2_2992 and Pf6N2E2_2993 are separated by 53 nucleotides
Pf6N2E2_2991 - decarboxylase, at 3,483,333 to 3,484,451
_2991
Pf6N2E2_2992 - Regulatory protein RecX, at 3,484,498 to 3,484,920
_2992
Pf6N2E2_2993 - RecA protein, at 3,484,974 to 3,486,026
_2993
Position (kb)
3484
3485 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3483.526 kb on + strand, within Pf6N2E2_2991 at 3483.526 kb on + strand, within Pf6N2E2_2991 at 3483.526 kb on + strand, within Pf6N2E2_2991 at 3483.526 kb on + strand, within Pf6N2E2_2991 at 3483.526 kb on + strand, within Pf6N2E2_2991 at 3483.527 kb on - strand, within Pf6N2E2_2991 at 3483.527 kb on - strand, within Pf6N2E2_2991 at 3483.527 kb on - strand, within Pf6N2E2_2991 at 3483.636 kb on + strand, within Pf6N2E2_2991 at 3483.637 kb on - strand, within Pf6N2E2_2991 at 3483.637 kb on - strand, within Pf6N2E2_2991 at 3483.637 kb on - strand, within Pf6N2E2_2991 at 3483.848 kb on + strand, within Pf6N2E2_2991 at 3483.848 kb on + strand, within Pf6N2E2_2991 at 3483.940 kb on + strand, within Pf6N2E2_2991 at 3483.940 kb on + strand, within Pf6N2E2_2991 at 3483.941 kb on - strand, within Pf6N2E2_2991 at 3483.941 kb on - strand, within Pf6N2E2_2991 at 3484.090 kb on - strand, within Pf6N2E2_2991 at 3484.192 kb on + strand, within Pf6N2E2_2991 at 3484.192 kb on + strand, within Pf6N2E2_2991 at 3484.192 kb on + strand, within Pf6N2E2_2991 at 3484.193 kb on - strand, within Pf6N2E2_2991 at 3484.269 kb on + strand, within Pf6N2E2_2991 at 3484.269 kb on + strand, within Pf6N2E2_2991 at 3484.269 kb on + strand, within Pf6N2E2_2991 at 3484.270 kb on - strand, within Pf6N2E2_2991 at 3484.270 kb on - strand, within Pf6N2E2_2991 at 3484.371 kb on + strand at 3484.371 kb on + strand at 3484.457 kb on + strand at 3484.481 kb on + strand at 3484.481 kb on + strand at 3484.482 kb on - strand at 3484.482 kb on - strand at 3484.482 kb on - strand at 3484.499 kb on + strand at 3484.606 kb on + strand, within Pf6N2E2_2992 at 3484.606 kb on + strand, within Pf6N2E2_2992 at 3484.607 kb on - strand, within Pf6N2E2_2992 at 3484.607 kb on - strand, within Pf6N2E2_2992 at 3484.763 kb on + strand, within Pf6N2E2_2992 at 3484.764 kb on - strand, within Pf6N2E2_2992 at 3484.872 kb on + strand, within Pf6N2E2_2992 at 3484.873 kb on - strand, within Pf6N2E2_2992 at 3484.873 kb on - strand, within Pf6N2E2_2992 at 3485.466 kb on + strand, within Pf6N2E2_2993 at 3485.816 kb on + strand, within Pf6N2E2_2993
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 3,483,526 + Pf6N2E2_2991 0.17 -1.4 3,483,526 + Pf6N2E2_2991 0.17 -0.5 3,483,526 + Pf6N2E2_2991 0.17 -1.0 3,483,526 + Pf6N2E2_2991 0.17 -2.4 3,483,526 + Pf6N2E2_2991 0.17 +2.6 3,483,527 - Pf6N2E2_2991 0.17 -1.2 3,483,527 - Pf6N2E2_2991 0.17 +1.8 3,483,527 - Pf6N2E2_2991 0.17 -1.2 3,483,636 + Pf6N2E2_2991 0.27 -0.9 3,483,637 - Pf6N2E2_2991 0.27 +1.5 3,483,637 - Pf6N2E2_2991 0.27 +1.2 3,483,637 - Pf6N2E2_2991 0.27 +0.3 3,483,848 + Pf6N2E2_2991 0.46 +0.5 3,483,848 + Pf6N2E2_2991 0.46 +0.7 3,483,940 + Pf6N2E2_2991 0.54 +1.2 3,483,940 + Pf6N2E2_2991 0.54 -0.6 3,483,941 - Pf6N2E2_2991 0.54 +0.1 3,483,941 - Pf6N2E2_2991 0.54 +1.9 3,484,090 - Pf6N2E2_2991 0.68 +0.9 3,484,192 + Pf6N2E2_2991 0.77 -0.5 3,484,192 + Pf6N2E2_2991 0.77 +0.6 3,484,192 + Pf6N2E2_2991 0.77 -0.3 3,484,193 - Pf6N2E2_2991 0.77 -2.0 3,484,269 + Pf6N2E2_2991 0.84 -0.7 3,484,269 + Pf6N2E2_2991 0.84 +1.1 3,484,269 + Pf6N2E2_2991 0.84 +0.2 3,484,270 - Pf6N2E2_2991 0.84 -1.7 3,484,270 - Pf6N2E2_2991 0.84 +0.5 3,484,371 + +0.3 3,484,371 + -1.5 3,484,457 + -2.6 3,484,481 + +1.5 3,484,481 + -1.1 3,484,482 - +0.6 3,484,482 - +1.4 3,484,482 - +1.0 3,484,499 + +0.3 3,484,606 + Pf6N2E2_2992 0.26 -0.7 3,484,606 + Pf6N2E2_2992 0.26 +0.3 3,484,607 - Pf6N2E2_2992 0.26 -1.2 3,484,607 - Pf6N2E2_2992 0.26 -2.4 3,484,763 + Pf6N2E2_2992 0.63 -2.8 3,484,764 - Pf6N2E2_2992 0.63 -2.1 3,484,872 + Pf6N2E2_2992 0.88 -0.9 3,484,873 - Pf6N2E2_2992 0.89 -2.5 3,484,873 - Pf6N2E2_2992 0.89 +0.9 3,485,466 + Pf6N2E2_2993 0.47 -2.1 3,485,816 + Pf6N2E2_2993 0.80 -1.4
Or see this region's nucleotide sequence