Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2973 and Pf6N2E2_2974 are separated by 214 nucleotides Pf6N2E2_2974 and Pf6N2E2_6114 are separated by 174 nucleotides Pf6N2E2_6114 and Pf6N2E2_6115 are separated by 223 nucleotides Pf6N2E2_6115 and Pf6N2E2_2975 are separated by 156 nucleotides
Pf6N2E2_2973 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,464,272 to 3,465,225
_2973
Pf6N2E2_2974 - DNA polymerase IV (EC 2.7.7.7), at 3,465,440 to 3,466,612
_2974
Pf6N2E2_6114 - tRNA-Met-CAT, at 3,466,787 to 3,466,860
_6114
Pf6N2E2_6115 - tRNA-Met-CAT, at 3,467,084 to 3,467,157
_6115
Pf6N2E2_2975 - membrane protein, putative, at 3,467,314 to 3,469,956
_2975
Position (kb)
3465
3466
3467 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3464.515 kb on + strand, within Pf6N2E2_2973 at 3464.525 kb on - strand, within Pf6N2E2_2973 at 3464.555 kb on - strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3465.023 kb on - strand, within Pf6N2E2_2973 at 3465.023 kb on - strand, within Pf6N2E2_2973 at 3465.314 kb on + strand at 3465.315 kb on - strand at 3465.315 kb on - strand at 3465.315 kb on - strand at 3465.316 kb on + strand at 3465.316 kb on + strand at 3465.316 kb on + strand at 3465.316 kb on + strand at 3465.317 kb on - strand at 3465.317 kb on - strand at 3465.317 kb on - strand at 3465.561 kb on + strand, within Pf6N2E2_2974 at 3465.561 kb on + strand, within Pf6N2E2_2974 at 3465.562 kb on - strand, within Pf6N2E2_2974 at 3465.562 kb on - strand, within Pf6N2E2_2974 at 3465.750 kb on + strand, within Pf6N2E2_2974 at 3465.750 kb on + strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3466.180 kb on - strand, within Pf6N2E2_2974 at 3466.218 kb on + strand, within Pf6N2E2_2974 at 3466.219 kb on - strand, within Pf6N2E2_2974 at 3466.219 kb on - strand, within Pf6N2E2_2974 at 3466.261 kb on - strand, within Pf6N2E2_2974 at 3466.345 kb on - strand, within Pf6N2E2_2974 at 3466.359 kb on + strand, within Pf6N2E2_2974 at 3466.360 kb on - strand, within Pf6N2E2_2974 at 3466.363 kb on + strand, within Pf6N2E2_2974 at 3466.363 kb on + strand, within Pf6N2E2_2974 at 3466.489 kb on + strand, within Pf6N2E2_2974 at 3466.489 kb on + strand, within Pf6N2E2_2974 at 3466.489 kb on + strand, within Pf6N2E2_2974 at 3466.490 kb on - strand, within Pf6N2E2_2974 at 3466.490 kb on - strand, within Pf6N2E2_2974 at 3466.490 kb on - strand, within Pf6N2E2_2974 at 3466.501 kb on + strand at 3466.501 kb on + strand at 3466.505 kb on + strand at 3466.505 kb on + strand at 3466.505 kb on + strand at 3466.505 kb on + strand at 3466.506 kb on - strand at 3466.506 kb on - strand at 3466.518 kb on + strand at 3466.519 kb on - strand at 3466.519 kb on - strand at 3466.519 kb on - strand at 3466.541 kb on + strand at 3466.558 kb on + strand at 3466.558 kb on + strand at 3466.559 kb on - strand at 3466.559 kb on - strand at 3466.559 kb on - strand at 3466.559 kb on - strand at 3466.559 kb on - strand at 3466.560 kb on + strand at 3466.560 kb on + strand at 3466.560 kb on + strand at 3466.560 kb on + strand at 3466.562 kb on + strand at 3466.959 kb on + strand at 3466.989 kb on + strand at 3466.989 kb on + strand at 3466.989 kb on + strand at 3467.018 kb on - strand at 3467.030 kb on + strand at 3467.163 kb on + strand at 3467.163 kb on + strand at 3467.163 kb on + strand at 3467.163 kb on + strand at 3467.224 kb on + strand at 3467.225 kb on - strand at 3467.242 kb on - strand at 3467.264 kb on - strand at 3467.264 kb on - strand at 3467.264 kb on - strand at 3467.296 kb on + strand at 3467.296 kb on + strand at 3467.350 kb on - strand at 3467.350 kb on - strand at 3467.418 kb on + strand at 3467.419 kb on - strand at 3467.419 kb on - strand at 3467.419 kb on - strand at 3467.419 kb on - strand at 3467.441 kb on + strand at 3467.441 kb on + strand at 3467.590 kb on + strand, within Pf6N2E2_2975
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 3,464,515 + Pf6N2E2_2973 0.25 -1.1 3,464,525 - Pf6N2E2_2973 0.27 +1.5 3,464,555 - Pf6N2E2_2973 0.30 -0.2 3,464,665 + Pf6N2E2_2973 0.41 +1.1 3,464,665 + Pf6N2E2_2973 0.41 +2.0 3,464,665 + Pf6N2E2_2973 0.41 -1.6 3,464,665 + Pf6N2E2_2973 0.41 +1.1 3,464,666 - Pf6N2E2_2973 0.41 +2.3 3,464,666 - Pf6N2E2_2973 0.41 -1.6 3,464,666 - Pf6N2E2_2973 0.41 +2.4 3,464,666 - Pf6N2E2_2973 0.41 -2.1 3,465,023 - Pf6N2E2_2973 0.79 +1.9 3,465,023 - Pf6N2E2_2973 0.79 +4.1 3,465,314 + -0.5 3,465,315 - -1.8 3,465,315 - -1.7 3,465,315 - +1.4 3,465,316 + -0.9 3,465,316 + -2.1 3,465,316 + +0.1 3,465,316 + -0.7 3,465,317 - +1.2 3,465,317 - -2.1 3,465,317 - -2.3 3,465,561 + Pf6N2E2_2974 0.10 -1.9 3,465,561 + Pf6N2E2_2974 0.10 +0.5 3,465,562 - Pf6N2E2_2974 0.10 +1.1 3,465,562 - Pf6N2E2_2974 0.10 -0.7 3,465,750 + Pf6N2E2_2974 0.26 -1.3 3,465,750 + Pf6N2E2_2974 0.26 -0.8 3,465,751 - Pf6N2E2_2974 0.27 +2.6 3,465,751 - Pf6N2E2_2974 0.27 +0.3 3,465,751 - Pf6N2E2_2974 0.27 +0.4 3,465,751 - Pf6N2E2_2974 0.27 -2.4 3,465,751 - Pf6N2E2_2974 0.27 -3.5 3,466,180 - Pf6N2E2_2974 0.63 +1.2 3,466,218 + Pf6N2E2_2974 0.66 -3.1 3,466,219 - Pf6N2E2_2974 0.66 -0.1 3,466,219 - Pf6N2E2_2974 0.66 -2.0 3,466,261 - Pf6N2E2_2974 0.70 +3.2 3,466,345 - Pf6N2E2_2974 0.77 +2.7 3,466,359 + Pf6N2E2_2974 0.78 -2.0 3,466,360 - Pf6N2E2_2974 0.78 +1.8 3,466,363 + Pf6N2E2_2974 0.79 +2.1 3,466,363 + Pf6N2E2_2974 0.79 -2.0 3,466,489 + Pf6N2E2_2974 0.89 +0.3 3,466,489 + Pf6N2E2_2974 0.89 -2.5 3,466,489 + Pf6N2E2_2974 0.89 +1.9 3,466,490 - Pf6N2E2_2974 0.90 +0.7 3,466,490 - Pf6N2E2_2974 0.90 +1.4 3,466,490 - Pf6N2E2_2974 0.90 -2.7 3,466,501 + -2.8 3,466,501 + +0.4 3,466,505 + +4.1 3,466,505 + -3.3 3,466,505 + -1.7 3,466,505 + +0.5 3,466,506 - -2.0 3,466,506 - +0.8 3,466,518 + -2.1 3,466,519 - -2.3 3,466,519 - +0.8 3,466,519 - -1.8 3,466,541 + -3.4 3,466,558 + +1.0 3,466,558 + +0.1 3,466,559 - +1.8 3,466,559 - -2.6 3,466,559 - +1.2 3,466,559 - -2.8 3,466,559 - +1.5 3,466,560 + +1.5 3,466,560 + +0.1 3,466,560 + -0.1 3,466,560 + +1.5 3,466,562 + +1.3 3,466,959 + +0.9 3,466,989 + +1.4 3,466,989 + +0.7 3,466,989 + +2.5 3,467,018 - +0.8 3,467,030 + -0.0 3,467,163 + +0.2 3,467,163 + +0.0 3,467,163 + +1.4 3,467,163 + +2.4 3,467,224 + -0.7 3,467,225 - +0.7 3,467,242 - -0.4 3,467,264 - -1.8 3,467,264 - +2.4 3,467,264 - +1.1 3,467,296 + +2.3 3,467,296 + +1.3 3,467,350 - +1.1 3,467,350 - -1.4 3,467,418 + +0.5 3,467,419 - -1.1 3,467,419 - +0.2 3,467,419 - +0.6 3,467,419 - -1.3 3,467,441 + -1.3 3,467,441 + +0.8 3,467,590 + Pf6N2E2_2975 0.10 -0.3
Or see this region's nucleotide sequence