Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_259 and Pf6N2E2_260 are separated by 56 nucleotides Pf6N2E2_260 and Pf6N2E2_261 are separated by 198 nucleotides
Pf6N2E2_259 - Transcriptional regulator, LysR family, at 323,206 to 324,084
_259
Pf6N2E2_260 - Regulator of cell morphogenesis and NO signaling, at 324,141 to 324,617
_260
Pf6N2E2_261 - Aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 324,816 to 325,925
_261
Position (kb) 324
325 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 323.194 kb on - strand at 323.194 kb on - strand at 323.620 kb on + strand, within Pf6N2E2_259 at 323.620 kb on + strand, within Pf6N2E2_259 at 323.620 kb on + strand, within Pf6N2E2_259 at 323.621 kb on - strand, within Pf6N2E2_259 at 323.698 kb on + strand, within Pf6N2E2_259 at 323.698 kb on + strand, within Pf6N2E2_259 at 323.699 kb on - strand, within Pf6N2E2_259 at 323.716 kb on + strand, within Pf6N2E2_259 at 323.914 kb on + strand, within Pf6N2E2_259 at 323.914 kb on + strand, within Pf6N2E2_259 at 323.914 kb on + strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 324.083 kb on - strand at 324.083 kb on - strand at 324.128 kb on + strand at 324.128 kb on + strand at 324.129 kb on - strand at 324.179 kb on - strand at 324.383 kb on + strand, within Pf6N2E2_260 at 324.406 kb on + strand, within Pf6N2E2_260 at 324.406 kb on + strand, within Pf6N2E2_260 at 324.407 kb on - strand, within Pf6N2E2_260 at 324.409 kb on + strand, within Pf6N2E2_260 at 324.409 kb on + strand, within Pf6N2E2_260 at 324.410 kb on - strand, within Pf6N2E2_260 at 324.617 kb on + strand at 324.617 kb on + strand at 324.617 kb on + strand at 324.617 kb on + strand at 324.617 kb on + strand at 324.617 kb on + strand at 324.617 kb on + strand at 324.618 kb on - strand at 324.618 kb on - strand at 324.618 kb on - strand at 324.618 kb on - strand at 324.621 kb on + strand at 324.621 kb on + strand at 324.621 kb on + strand at 324.621 kb on + strand at 324.621 kb on + strand at 324.635 kb on + strand at 325.034 kb on - strand, within Pf6N2E2_261 at 325.228 kb on + strand, within Pf6N2E2_261 at 325.228 kb on + strand, within Pf6N2E2_261 at 325.229 kb on - strand, within Pf6N2E2_261 at 325.408 kb on - strand, within Pf6N2E2_261 at 325.507 kb on + strand, within Pf6N2E2_261 at 325.507 kb on + strand, within Pf6N2E2_261 at 325.508 kb on - strand, within Pf6N2E2_261 at 325.609 kb on - strand, within Pf6N2E2_261 at 325.609 kb on - strand, within Pf6N2E2_261
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 323,194 - -1.7 323,194 - -2.0 323,620 + Pf6N2E2_259 0.47 -0.6 323,620 + Pf6N2E2_259 0.47 +1.4 323,620 + Pf6N2E2_259 0.47 +0.8 323,621 - Pf6N2E2_259 0.47 -2.3 323,698 + Pf6N2E2_259 0.56 -1.1 323,698 + Pf6N2E2_259 0.56 -1.3 323,699 - Pf6N2E2_259 0.56 +2.4 323,716 + Pf6N2E2_259 0.58 +1.0 323,914 + Pf6N2E2_259 0.81 -1.0 323,914 + Pf6N2E2_259 0.81 +0.5 323,914 + Pf6N2E2_259 0.81 -1.6 323,915 - Pf6N2E2_259 0.81 -2.7 323,915 - Pf6N2E2_259 0.81 -0.3 323,915 - Pf6N2E2_259 0.81 -0.7 323,915 - Pf6N2E2_259 0.81 -2.3 323,915 - Pf6N2E2_259 0.81 -0.1 324,083 - +0.7 324,083 - +0.1 324,128 + +0.2 324,128 + -2.0 324,129 - +0.3 324,179 - -0.8 324,383 + Pf6N2E2_260 0.51 +0.6 324,406 + Pf6N2E2_260 0.56 -0.3 324,406 + Pf6N2E2_260 0.56 -1.9 324,407 - Pf6N2E2_260 0.56 -0.7 324,409 + Pf6N2E2_260 0.56 +0.8 324,409 + Pf6N2E2_260 0.56 -0.5 324,410 - Pf6N2E2_260 0.56 -1.8 324,617 + +0.8 324,617 + -0.9 324,617 + +0.1 324,617 + +1.7 324,617 + +1.3 324,617 + -3.1 324,617 + -1.9 324,618 - -3.4 324,618 - +0.6 324,618 - -0.3 324,618 - -0.3 324,621 + +2.5 324,621 + +1.5 324,621 + +1.4 324,621 + -1.3 324,621 + +1.0 324,635 + -2.4 325,034 - Pf6N2E2_261 0.20 +1.3 325,228 + Pf6N2E2_261 0.37 +0.4 325,228 + Pf6N2E2_261 0.37 +0.3 325,229 - Pf6N2E2_261 0.37 +0.3 325,408 - Pf6N2E2_261 0.53 -2.3 325,507 + Pf6N2E2_261 0.62 +0.3 325,507 + Pf6N2E2_261 0.62 +0.6 325,508 - Pf6N2E2_261 0.62 -1.9 325,609 - Pf6N2E2_261 0.71 +0.7 325,609 - Pf6N2E2_261 0.71 -0.7
Or see this region's nucleotide sequence