Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_254 and Pf6N2E2_255 overlap by 4 nucleotides Pf6N2E2_255 and Pf6N2E2_256 overlap by 8 nucleotides Pf6N2E2_256 and Pf6N2E2_257 are separated by 118 nucleotides
Pf6N2E2_254 - Transcriptional regulator, LysR family, at 318,247 to 319,194
_254
Pf6N2E2_255 - DoxX family protein, at 319,191 to 319,604
_255
Pf6N2E2_256 - putative membrane protein, at 319,597 to 320,235
_256
Pf6N2E2_257 - Exoenzymes regulatory protein AepA precursor, at 320,354 to 322,192
_257
Position (kb) 319
320
321 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 318.712 kb on + strand, within Pf6N2E2_254 at 318.712 kb on + strand, within Pf6N2E2_254 at 318.817 kb on + strand, within Pf6N2E2_254 at 318.818 kb on - strand, within Pf6N2E2_254 at 318.818 kb on - strand, within Pf6N2E2_254 at 318.818 kb on - strand, within Pf6N2E2_254 at 318.818 kb on - strand, within Pf6N2E2_254 at 318.988 kb on + strand, within Pf6N2E2_254 at 319.299 kb on + strand, within Pf6N2E2_255 at 319.299 kb on + strand, within Pf6N2E2_255 at 319.299 kb on + strand, within Pf6N2E2_255 at 319.300 kb on - strand, within Pf6N2E2_255 at 319.300 kb on - strand, within Pf6N2E2_255 at 319.525 kb on + strand, within Pf6N2E2_255 at 319.525 kb on + strand, within Pf6N2E2_255 at 319.526 kb on - strand, within Pf6N2E2_255 at 319.526 kb on - strand, within Pf6N2E2_255 at 319.537 kb on + strand, within Pf6N2E2_255 at 319.537 kb on + strand, within Pf6N2E2_255 at 319.538 kb on - strand, within Pf6N2E2_255 at 319.538 kb on - strand, within Pf6N2E2_255 at 319.733 kb on + strand, within Pf6N2E2_256 at 319.734 kb on - strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.143 kb on + strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.333 kb on - strand at 320.393 kb on - strand at 320.790 kb on - strand, within Pf6N2E2_257 at 320.834 kb on + strand, within Pf6N2E2_257 at 320.975 kb on + strand, within Pf6N2E2_257 at 321.077 kb on + strand, within Pf6N2E2_257 at 321.078 kb on - strand, within Pf6N2E2_257 at 321.131 kb on + strand, within Pf6N2E2_257 at 321.132 kb on - strand, within Pf6N2E2_257
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 318,712 + Pf6N2E2_254 0.49 +1.5 318,712 + Pf6N2E2_254 0.49 +0.8 318,817 + Pf6N2E2_254 0.60 +2.5 318,818 - Pf6N2E2_254 0.60 +0.1 318,818 - Pf6N2E2_254 0.60 +2.1 318,818 - Pf6N2E2_254 0.60 +0.9 318,818 - Pf6N2E2_254 0.60 -3.0 318,988 + Pf6N2E2_254 0.78 -0.6 319,299 + Pf6N2E2_255 0.26 -1.5 319,299 + Pf6N2E2_255 0.26 +1.7 319,299 + Pf6N2E2_255 0.26 -0.3 319,300 - Pf6N2E2_255 0.26 -2.8 319,300 - Pf6N2E2_255 0.26 -0.2 319,525 + Pf6N2E2_255 0.81 -2.8 319,525 + Pf6N2E2_255 0.81 +1.1 319,526 - Pf6N2E2_255 0.81 -1.1 319,526 - Pf6N2E2_255 0.81 -0.8 319,537 + Pf6N2E2_255 0.84 -1.0 319,537 + Pf6N2E2_255 0.84 +1.2 319,538 - Pf6N2E2_255 0.84 +2.2 319,538 - Pf6N2E2_255 0.84 +1.8 319,733 + Pf6N2E2_256 0.21 +0.7 319,734 - Pf6N2E2_256 0.21 +0.8 320,092 + Pf6N2E2_256 0.77 -1.7 320,092 + Pf6N2E2_256 0.77 -1.3 320,092 + Pf6N2E2_256 0.77 +0.9 320,092 + Pf6N2E2_256 0.77 -1.8 320,093 - Pf6N2E2_256 0.78 +0.1 320,093 - Pf6N2E2_256 0.78 +0.1 320,093 - Pf6N2E2_256 0.78 -0.7 320,093 - Pf6N2E2_256 0.78 +0.6 320,143 + Pf6N2E2_256 0.85 -1.9 320,144 - Pf6N2E2_256 0.86 +1.9 320,144 - Pf6N2E2_256 0.86 -1.8 320,144 - Pf6N2E2_256 0.86 +0.2 320,144 - Pf6N2E2_256 0.86 -0.2 320,144 - Pf6N2E2_256 0.86 -3.0 320,333 - -0.0 320,393 - -2.0 320,790 - Pf6N2E2_257 0.24 -2.7 320,834 + Pf6N2E2_257 0.26 -2.2 320,975 + Pf6N2E2_257 0.34 +1.8 321,077 + Pf6N2E2_257 0.39 +1.3 321,078 - Pf6N2E2_257 0.39 +2.0 321,131 + Pf6N2E2_257 0.42 -0.3 321,132 - Pf6N2E2_257 0.42 +0.9
Or see this region's nucleotide sequence