Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2544 and Pf6N2E2_2545 are separated by 181 nucleotides Pf6N2E2_2545 and Pf6N2E2_2546 overlap by 12 nucleotides Pf6N2E2_2546 and Pf6N2E2_2547 are separated by 151 nucleotides Pf6N2E2_2547 and Pf6N2E2_2548 are separated by 12 nucleotides
Pf6N2E2_2544 - Deblocking aminopeptidase (EC 3.4.11.-) @ Cyanophycinase 2 (EC 3.4.15.6), at 3,006,167 to 3,007,357
_2544
Pf6N2E2_2545 - FIG00953242: hypothetical protein, at 3,007,539 to 3,007,766
_2545
Pf6N2E2_2546 - Carbon storage regulator, at 3,007,755 to 3,007,955
_2546
Pf6N2E2_2547 - Protein of unknown function UPF0060, at 3,008,107 to 3,008,484
_2547
Pf6N2E2_2548 - Oxidoreductase, short chain dehydrogenase/reductase family, at 3,008,497 to 3,009,312
_2548
Position (kb)
3008
3009 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3007.202 kb on + strand, within Pf6N2E2_2544 at 3007.202 kb on + strand, within Pf6N2E2_2544 at 3007.202 kb on + strand, within Pf6N2E2_2544 at 3007.203 kb on - strand, within Pf6N2E2_2544 at 3007.203 kb on - strand, within Pf6N2E2_2544 at 3007.203 kb on - strand, within Pf6N2E2_2544 at 3007.203 kb on - strand, within Pf6N2E2_2544 at 3007.517 kb on + strand at 3007.518 kb on - strand at 3007.518 kb on - strand at 3007.518 kb on - strand at 3007.518 kb on - strand at 3007.756 kb on + strand at 3007.757 kb on - strand at 3007.813 kb on + strand, within Pf6N2E2_2546 at 3007.813 kb on + strand, within Pf6N2E2_2546 at 3007.813 kb on + strand, within Pf6N2E2_2546 at 3007.904 kb on + strand, within Pf6N2E2_2546 at 3007.904 kb on + strand, within Pf6N2E2_2546 at 3007.932 kb on + strand, within Pf6N2E2_2546 at 3007.932 kb on + strand, within Pf6N2E2_2546 at 3007.947 kb on + strand at 3007.947 kb on + strand at 3007.947 kb on + strand at 3007.947 kb on + strand at 3007.947 kb on + strand at 3007.948 kb on - strand at 3007.948 kb on - strand at 3007.948 kb on - strand at 3008.044 kb on - strand at 3008.044 kb on - strand at 3008.104 kb on - strand at 3008.104 kb on - strand at 3008.238 kb on + strand, within Pf6N2E2_2547 at 3008.238 kb on + strand, within Pf6N2E2_2547 at 3008.238 kb on + strand, within Pf6N2E2_2547 at 3008.238 kb on + strand, within Pf6N2E2_2547 at 3008.238 kb on + strand, within Pf6N2E2_2547 at 3008.238 kb on + strand, within Pf6N2E2_2547 at 3008.239 kb on - strand, within Pf6N2E2_2547 at 3008.239 kb on - strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.241 kb on - strand, within Pf6N2E2_2547 at 3008.241 kb on - strand, within Pf6N2E2_2547 at 3008.241 kb on - strand, within Pf6N2E2_2547 at 3008.241 kb on - strand, within Pf6N2E2_2547 at 3008.253 kb on - strand, within Pf6N2E2_2547 at 3008.261 kb on + strand, within Pf6N2E2_2547 at 3008.276 kb on + strand, within Pf6N2E2_2547 at 3008.276 kb on + strand, within Pf6N2E2_2547 at 3008.277 kb on - strand, within Pf6N2E2_2547 at 3008.385 kb on - strand, within Pf6N2E2_2547 at 3008.429 kb on + strand, within Pf6N2E2_2547 at 3008.429 kb on + strand, within Pf6N2E2_2547 at 3008.429 kb on + strand, within Pf6N2E2_2547 at 3008.429 kb on + strand, within Pf6N2E2_2547 at 3008.430 kb on - strand, within Pf6N2E2_2547 at 3008.430 kb on - strand, within Pf6N2E2_2547 at 3008.430 kb on - strand, within Pf6N2E2_2547 at 3008.430 kb on - strand, within Pf6N2E2_2547 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.657 kb on + strand, within Pf6N2E2_2548 at 3008.657 kb on + strand, within Pf6N2E2_2548 at 3008.657 kb on + strand, within Pf6N2E2_2548 at 3008.658 kb on - strand, within Pf6N2E2_2548 at 3008.658 kb on - strand, within Pf6N2E2_2548 at 3008.658 kb on - strand, within Pf6N2E2_2548 at 3008.782 kb on + strand, within Pf6N2E2_2548 at 3008.782 kb on + strand, within Pf6N2E2_2548 at 3008.782 kb on + strand, within Pf6N2E2_2548 at 3008.782 kb on + strand, within Pf6N2E2_2548 at 3008.782 kb on + strand, within Pf6N2E2_2548 at 3008.783 kb on - strand, within Pf6N2E2_2548 at 3008.783 kb on - strand, within Pf6N2E2_2548 at 3008.783 kb on - strand, within Pf6N2E2_2548 at 3008.783 kb on - strand, within Pf6N2E2_2548 at 3008.788 kb on + strand, within Pf6N2E2_2548 at 3008.789 kb on - strand, within Pf6N2E2_2548 at 3008.789 kb on - strand, within Pf6N2E2_2548 at 3008.789 kb on - strand, within Pf6N2E2_2548 at 3008.789 kb on - strand, within Pf6N2E2_2548 at 3008.864 kb on + strand, within Pf6N2E2_2548 at 3008.864 kb on + strand, within Pf6N2E2_2548 at 3008.865 kb on - strand, within Pf6N2E2_2548 at 3008.865 kb on - strand, within Pf6N2E2_2548 at 3008.865 kb on - strand, within Pf6N2E2_2548 at 3009.125 kb on + strand, within Pf6N2E2_2548 at 3009.125 kb on + strand, within Pf6N2E2_2548 at 3009.179 kb on - strand, within Pf6N2E2_2548 at 3009.179 kb on - strand, within Pf6N2E2_2548 at 3009.179 kb on - strand, within Pf6N2E2_2548 at 3009.179 kb on - strand, within Pf6N2E2_2548 at 3009.312 kb on + strand at 3009.342 kb on + strand at 3009.342 kb on + strand at 3009.353 kb on + strand at 3009.440 kb on + strand at 3009.462 kb on + strand at 3009.462 kb on + strand at 3009.463 kb on - strand at 3009.463 kb on - strand at 3009.463 kb on - strand at 3009.465 kb on + strand at 3009.465 kb on + strand at 3009.466 kb on - strand at 3009.466 kb on - strand at 3009.480 kb on + strand at 3009.481 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 3,007,202 + Pf6N2E2_2544 0.87 -2.2 3,007,202 + Pf6N2E2_2544 0.87 +1.6 3,007,202 + Pf6N2E2_2544 0.87 -0.7 3,007,203 - Pf6N2E2_2544 0.87 -0.2 3,007,203 - Pf6N2E2_2544 0.87 -0.7 3,007,203 - Pf6N2E2_2544 0.87 +1.7 3,007,203 - Pf6N2E2_2544 0.87 +0.0 3,007,517 + +0.2 3,007,518 - +2.2 3,007,518 - -0.7 3,007,518 - -0.6 3,007,518 - -1.5 3,007,756 + -1.1 3,007,757 - -0.3 3,007,813 + Pf6N2E2_2546 0.29 -1.6 3,007,813 + Pf6N2E2_2546 0.29 -2.0 3,007,813 + Pf6N2E2_2546 0.29 +1.2 3,007,904 + Pf6N2E2_2546 0.74 -1.0 3,007,904 + Pf6N2E2_2546 0.74 +0.9 3,007,932 + Pf6N2E2_2546 0.88 +0.1 3,007,932 + Pf6N2E2_2546 0.88 +0.9 3,007,947 + -1.3 3,007,947 + -1.6 3,007,947 + +1.0 3,007,947 + -0.1 3,007,947 + +2.3 3,007,948 - +1.9 3,007,948 - -2.3 3,007,948 - +1.9 3,008,044 - +0.9 3,008,044 - -1.5 3,008,104 - -1.4 3,008,104 - -1.5 3,008,238 + Pf6N2E2_2547 0.35 -0.1 3,008,238 + Pf6N2E2_2547 0.35 +0.0 3,008,238 + Pf6N2E2_2547 0.35 -1.2 3,008,238 + Pf6N2E2_2547 0.35 +1.2 3,008,238 + Pf6N2E2_2547 0.35 +0.1 3,008,238 + Pf6N2E2_2547 0.35 -3.0 3,008,239 - Pf6N2E2_2547 0.35 -0.1 3,008,239 - Pf6N2E2_2547 0.35 +3.2 3,008,240 + Pf6N2E2_2547 0.35 +0.7 3,008,240 + Pf6N2E2_2547 0.35 -2.2 3,008,240 + Pf6N2E2_2547 0.35 +0.2 3,008,240 + Pf6N2E2_2547 0.35 +0.7 3,008,240 + Pf6N2E2_2547 0.35 +1.3 3,008,240 + Pf6N2E2_2547 0.35 -0.1 3,008,240 + Pf6N2E2_2547 0.35 -0.6 3,008,240 + Pf6N2E2_2547 0.35 +0.1 3,008,240 + Pf6N2E2_2547 0.35 +0.9 3,008,240 + Pf6N2E2_2547 0.35 -2.5 3,008,241 - Pf6N2E2_2547 0.35 -1.1 3,008,241 - Pf6N2E2_2547 0.35 -0.0 3,008,241 - Pf6N2E2_2547 0.35 -1.7 3,008,241 - Pf6N2E2_2547 0.35 -2.5 3,008,253 - Pf6N2E2_2547 0.39 -0.1 3,008,261 + Pf6N2E2_2547 0.41 +0.6 3,008,276 + Pf6N2E2_2547 0.45 -3.1 3,008,276 + Pf6N2E2_2547 0.45 -0.5 3,008,277 - Pf6N2E2_2547 0.45 -1.4 3,008,385 - Pf6N2E2_2547 0.74 +0.2 3,008,429 + Pf6N2E2_2547 0.85 -0.5 3,008,429 + Pf6N2E2_2547 0.85 +1.6 3,008,429 + Pf6N2E2_2547 0.85 +0.5 3,008,429 + Pf6N2E2_2547 0.85 -0.1 3,008,430 - Pf6N2E2_2547 0.85 +0.9 3,008,430 - Pf6N2E2_2547 0.85 -2.7 3,008,430 - Pf6N2E2_2547 0.85 +3.2 3,008,430 - Pf6N2E2_2547 0.85 +1.9 3,008,651 + Pf6N2E2_2548 0.19 -2.1 3,008,651 + Pf6N2E2_2548 0.19 -2.5 3,008,651 + -0.5 3,008,651 + Pf6N2E2_2548 0.19 +2.3 3,008,651 + Pf6N2E2_2548 0.19 +0.5 3,008,651 + Pf6N2E2_2548 0.19 -0.4 3,008,651 + Pf6N2E2_2548 0.19 +0.5 3,008,651 + Pf6N2E2_2548 0.19 -1.3 3,008,651 + Pf6N2E2_2548 0.19 -1.0 3,008,652 - Pf6N2E2_2548 0.19 +0.9 3,008,652 - Pf6N2E2_2548 0.19 -1.6 3,008,652 - Pf6N2E2_2548 0.19 -0.2 3,008,652 - Pf6N2E2_2548 0.19 -1.5 3,008,652 - Pf6N2E2_2548 0.19 -1.8 3,008,652 - Pf6N2E2_2548 0.19 -0.5 3,008,652 - Pf6N2E2_2548 0.19 +1.5 3,008,657 + Pf6N2E2_2548 0.20 -0.1 3,008,657 + Pf6N2E2_2548 0.20 +0.7 3,008,657 + Pf6N2E2_2548 0.20 -0.7 3,008,658 - Pf6N2E2_2548 0.20 -3.4 3,008,658 - Pf6N2E2_2548 0.20 +0.9 3,008,658 - Pf6N2E2_2548 0.20 -2.3 3,008,782 + Pf6N2E2_2548 0.35 -0.0 3,008,782 + Pf6N2E2_2548 0.35 -2.2 3,008,782 + Pf6N2E2_2548 0.35 -0.5 3,008,782 + Pf6N2E2_2548 0.35 -3.0 3,008,782 + Pf6N2E2_2548 0.35 +0.9 3,008,783 - Pf6N2E2_2548 0.35 +0.7 3,008,783 - Pf6N2E2_2548 0.35 -1.0 3,008,783 - Pf6N2E2_2548 0.35 -2.1 3,008,783 - Pf6N2E2_2548 0.35 -1.0 3,008,788 + Pf6N2E2_2548 0.36 -2.5 3,008,789 - Pf6N2E2_2548 0.36 +1.4 3,008,789 - Pf6N2E2_2548 0.36 -2.7 3,008,789 - Pf6N2E2_2548 0.36 +1.2 3,008,789 - Pf6N2E2_2548 0.36 +1.9 3,008,864 + Pf6N2E2_2548 0.45 -2.3 3,008,864 + Pf6N2E2_2548 0.45 +0.7 3,008,865 - Pf6N2E2_2548 0.45 +0.2 3,008,865 - Pf6N2E2_2548 0.45 +0.1 3,008,865 - Pf6N2E2_2548 0.45 -3.0 3,009,125 + Pf6N2E2_2548 0.77 +0.9 3,009,125 + Pf6N2E2_2548 0.77 -1.1 3,009,179 - Pf6N2E2_2548 0.84 +2.0 3,009,179 - Pf6N2E2_2548 0.84 -1.0 3,009,179 - Pf6N2E2_2548 0.84 -1.1 3,009,179 - Pf6N2E2_2548 0.84 -1.1 3,009,312 + +0.9 3,009,342 + +0.9 3,009,342 + -0.5 3,009,353 + +0.2 3,009,440 + -0.4 3,009,462 + +0.7 3,009,462 + +0.8 3,009,463 - +0.5 3,009,463 - +0.5 3,009,463 - +2.1 3,009,465 + +0.8 3,009,465 + +1.0 3,009,466 - -1.1 3,009,466 - -0.9 3,009,480 + +1.2 3,009,481 - +0.7
Or see this region's nucleotide sequence