Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_250

Experiment: deoxycholate 0.0125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_249 and Pf6N2E2_250 are separated by 262 nucleotidesPf6N2E2_250 and Pf6N2E2_251 are separated by 184 nucleotides Pf6N2E2_249 - Pirin, at 310,575 to 311,297 _249 Pf6N2E2_250 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 311,560 to 312,495 _250 Pf6N2E2_251 - transcriptional regulator, at 312,680 to 314,167 _251 Position (kb) 311 312 313Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 310.699 kb on - strand, within Pf6N2E2_249at 310.738 kb on + strand, within Pf6N2E2_249at 310.738 kb on + strand, within Pf6N2E2_249at 310.739 kb on - strand, within Pf6N2E2_249at 310.739 kb on - strand, within Pf6N2E2_249at 310.739 kb on - strand, within Pf6N2E2_249at 310.739 kb on - strand, within Pf6N2E2_249at 310.744 kb on + strand, within Pf6N2E2_249at 310.745 kb on - strand, within Pf6N2E2_249at 310.745 kb on - strand, within Pf6N2E2_249at 310.802 kb on - strand, within Pf6N2E2_249at 311.098 kb on + strand, within Pf6N2E2_249at 311.098 kb on + strand, within Pf6N2E2_249at 311.098 kb on + strand, within Pf6N2E2_249at 311.099 kb on - strand, within Pf6N2E2_249at 311.460 kb on + strandat 311.460 kb on + strandat 311.460 kb on + strandat 311.461 kb on - strandat 311.461 kb on - strandat 311.512 kb on + strandat 311.512 kb on + strandat 311.513 kb on - strandat 311.513 kb on - strandat 311.513 kb on - strandat 311.513 kb on - strandat 311.513 kb on - strandat 311.514 kb on + strandat 311.632 kb on + strandat 311.753 kb on - strand, within Pf6N2E2_250at 311.788 kb on + strand, within Pf6N2E2_250at 311.788 kb on + strand, within Pf6N2E2_250at 311.788 kb on + strand, within Pf6N2E2_250at 311.788 kb on + strand, within Pf6N2E2_250at 311.933 kb on - strand, within Pf6N2E2_250at 311.987 kb on + strand, within Pf6N2E2_250at 311.987 kb on + strand, within Pf6N2E2_250at 311.987 kb on + strand, within Pf6N2E2_250at 311.987 kb on + strand, within Pf6N2E2_250at 312.578 kb on + strandat 312.619 kb on - strandat 312.653 kb on + strandat 312.839 kb on + strand, within Pf6N2E2_251at 312.962 kb on + strand, within Pf6N2E2_251at 312.962 kb on + strandat 312.962 kb on + strand, within Pf6N2E2_251at 312.963 kb on - strand, within Pf6N2E2_251at 312.989 kb on + strand, within Pf6N2E2_251

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Per-strain Table

Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M
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310,699 - Pf6N2E2_249 0.17 -2.3
310,738 + Pf6N2E2_249 0.23 +0.1
310,738 + Pf6N2E2_249 0.23 -2.7
310,739 - Pf6N2E2_249 0.23 +2.2
310,739 - Pf6N2E2_249 0.23 -2.2
310,739 - Pf6N2E2_249 0.23 -1.1
310,739 - Pf6N2E2_249 0.23 -1.2
310,744 + Pf6N2E2_249 0.23 +1.5
310,745 - Pf6N2E2_249 0.24 +1.2
310,745 - Pf6N2E2_249 0.24 +1.2
310,802 - Pf6N2E2_249 0.31 -2.8
311,098 + Pf6N2E2_249 0.72 +0.6
311,098 + Pf6N2E2_249 0.72 +0.2
311,098 + Pf6N2E2_249 0.72 -1.8
311,099 - Pf6N2E2_249 0.72 +0.5
311,460 + +1.3
311,460 + +1.0
311,460 + -2.7
311,461 - -2.3
311,461 - -1.3
311,512 + +2.1
311,512 + +2.8
311,513 - +0.7
311,513 - +1.7
311,513 - -0.7
311,513 - +0.6
311,513 - +0.6
311,514 + -1.5
311,632 + -2.3
311,753 - Pf6N2E2_250 0.21 -1.7
311,788 + Pf6N2E2_250 0.24 +1.7
311,788 + Pf6N2E2_250 0.24 +1.4
311,788 + Pf6N2E2_250 0.24 +0.7
311,788 + Pf6N2E2_250 0.24 -0.8
311,933 - Pf6N2E2_250 0.40 +1.9
311,987 + Pf6N2E2_250 0.46 +2.2
311,987 + Pf6N2E2_250 0.46 -2.7
311,987 + Pf6N2E2_250 0.46 +0.8
311,987 + Pf6N2E2_250 0.46 -0.9
312,578 + +0.9
312,619 - -1.9
312,653 + -2.3
312,839 + Pf6N2E2_251 0.11 +1.7
312,962 + Pf6N2E2_251 0.19 +0.9
312,962 + -1.5
312,962 + Pf6N2E2_251 0.19 -2.2
312,963 - Pf6N2E2_251 0.19 +1.5
312,989 + Pf6N2E2_251 0.21 +0.9

Or see this region's nucleotide sequence