Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1 and Pf6N2E2_2 are separated by 145 nucleotides Pf6N2E2_2 and Pf6N2E2_3 are separated by 273 nucleotides
Pf6N2E2_1 - Trans-aconitate 2-methyltransferase (EC 2.1.1.144), at 90 to 860
_1
Pf6N2E2_2 - FIG057993:Thioesterase involved in non-ribosomal peptide biosynthesis, at 1,006 to 1,740
_2
Pf6N2E2_3 - Non-ribosomal peptide synthetase modules, pyoverdine, at 2,014 to 14,454
_3
Position (kb) 1
2 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 0.027 kb on + strand at 0.028 kb on - strand at 0.035 kb on - strand at 0.035 kb on - strand at 0.158 kb on - strand at 0.181 kb on + strand, within Pf6N2E2_1 at 0.182 kb on - strand, within Pf6N2E2_1 at 0.193 kb on + strand, within Pf6N2E2_1 at 0.298 kb on + strand, within Pf6N2E2_1 at 0.349 kb on + strand, within Pf6N2E2_1 at 0.353 kb on - strand, within Pf6N2E2_1 at 0.353 kb on - strand, within Pf6N2E2_1 at 0.369 kb on + strand, within Pf6N2E2_1 at 0.370 kb on - strand, within Pf6N2E2_1 at 0.370 kb on - strand, within Pf6N2E2_1 at 0.838 kb on + strand at 0.839 kb on - strand at 0.839 kb on - strand at 0.839 kb on - strand at 0.902 kb on + strand at 0.902 kb on + strand at 0.902 kb on + strand at 0.903 kb on - strand at 0.904 kb on + strand at 0.904 kb on + strand at 0.904 kb on + strand at 0.904 kb on + strand at 0.905 kb on - strand at 0.905 kb on - strand at 0.948 kb on - strand at 0.955 kb on + strand at 0.955 kb on + strand at 1.037 kb on - strand at 1.060 kb on + strand at 1.060 kb on + strand at 1.060 kb on + strand at 1.061 kb on - strand at 1.349 kb on - strand, within Pf6N2E2_2 at 1.430 kb on + strand, within Pf6N2E2_2 at 1.431 kb on - strand, within Pf6N2E2_2 at 1.431 kb on - strand, within Pf6N2E2_2 at 1.431 kb on - strand, within Pf6N2E2_2 at 1.960 kb on - strand at 2.173 kb on + strand at 2.227 kb on + strand at 2.228 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 27 + +0.6 28 - +1.2 35 - +0.2 35 - +1.1 158 - +1.2 181 + Pf6N2E2_1 0.12 -2.6 182 - Pf6N2E2_1 0.12 +1.1 193 + Pf6N2E2_1 0.13 +0.9 298 + Pf6N2E2_1 0.27 +1.5 349 + Pf6N2E2_1 0.34 +1.6 353 - Pf6N2E2_1 0.34 +0.3 353 - Pf6N2E2_1 0.34 +0.5 369 + Pf6N2E2_1 0.36 -1.7 370 - Pf6N2E2_1 0.36 -1.8 370 - Pf6N2E2_1 0.36 +1.8 838 + +2.6 839 - +0.5 839 - +3.0 839 - +1.1 902 + -0.2 902 + +1.3 902 + -2.9 903 - -1.4 904 + -0.1 904 + +0.8 904 + +2.1 904 + +1.9 905 - -1.6 905 - -0.2 948 - +1.4 955 + -0.2 955 + -0.3 1,037 - -1.7 1,060 + -2.1 1,060 + +1.1 1,060 + +1.8 1,061 - +3.0 1,349 - Pf6N2E2_2 0.47 -0.3 1,430 + Pf6N2E2_2 0.58 -0.3 1,431 - Pf6N2E2_2 0.58 -1.1 1,431 - Pf6N2E2_2 0.58 -2.6 1,431 - Pf6N2E2_2 0.58 -2.3 1,960 - +0.9 2,173 + -0.2 2,227 + +1.3 2,228 - +1.8
Or see this region's nucleotide sequence