Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1977 and Pf6N2E2_1978 are separated by 8 nucleotides Pf6N2E2_1978 and Pf6N2E2_1979 are separated by 461 nucleotides Pf6N2E2_1979 and Pf6N2E2_1980 are separated by 204 nucleotides Pf6N2E2_1980 and Pf6N2E2_1981 are separated by 122 nucleotides
Pf6N2E2_1977 - DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster, at 2,342,473 to 2,344,023
_1977
Pf6N2E2_1978 - DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster, at 2,344,032 to 2,344,559
_1978
Pf6N2E2_1979 - Cytosine-specific DNA methyltransferase (EC 2.1.1.37), at 2,345,021 to 2,346,277
_1979
Pf6N2E2_1980 - hypothetical protein, at 2,346,482 to 2,346,886
_1980
Pf6N2E2_1981 - FIG051360: Periplasmic protein TonB, links inner and outer membranes, at 2,347,009 to 2,347,491
_1981
Position (kb)
2345
2346
2347 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2344.124 kb on + strand, within Pf6N2E2_1978 at 2344.152 kb on + strand, within Pf6N2E2_1978 at 2344.153 kb on - strand, within Pf6N2E2_1978 at 2344.153 kb on - strand, within Pf6N2E2_1978 at 2344.158 kb on + strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.659 kb on - strand at 2344.659 kb on - strand at 2344.749 kb on + strand at 2344.749 kb on + strand at 2344.749 kb on + strand at 2344.750 kb on - strand at 2344.750 kb on - strand at 2344.750 kb on - strand at 2344.750 kb on - strand at 2345.048 kb on + strand at 2345.101 kb on - strand at 2345.130 kb on + strand at 2345.131 kb on - strand at 2345.175 kb on + strand, within Pf6N2E2_1979 at 2345.176 kb on - strand, within Pf6N2E2_1979 at 2345.180 kb on - strand, within Pf6N2E2_1979 at 2345.180 kb on - strand, within Pf6N2E2_1979 at 2345.650 kb on - strand, within Pf6N2E2_1979 at 2345.701 kb on - strand, within Pf6N2E2_1979 at 2345.745 kb on + strand, within Pf6N2E2_1979 at 2345.745 kb on + strand, within Pf6N2E2_1979 at 2345.745 kb on + strand, within Pf6N2E2_1979 at 2345.746 kb on - strand, within Pf6N2E2_1979 at 2345.746 kb on - strand, within Pf6N2E2_1979 at 2345.746 kb on - strand, within Pf6N2E2_1979 at 2345.746 kb on - strand, within Pf6N2E2_1979 at 2345.919 kb on + strand, within Pf6N2E2_1979 at 2345.920 kb on - strand, within Pf6N2E2_1979 at 2345.920 kb on - strand, within Pf6N2E2_1979 at 2346.023 kb on + strand, within Pf6N2E2_1979 at 2346.023 kb on + strand, within Pf6N2E2_1979 at 2346.024 kb on - strand, within Pf6N2E2_1979 at 2346.024 kb on - strand, within Pf6N2E2_1979 at 2346.024 kb on - strand, within Pf6N2E2_1979 at 2346.024 kb on - strand, within Pf6N2E2_1979 at 2346.150 kb on + strand, within Pf6N2E2_1979 at 2346.150 kb on + strand, within Pf6N2E2_1979 at 2346.211 kb on + strand at 2346.237 kb on + strand at 2346.237 kb on + strand at 2346.237 kb on + strand at 2346.238 kb on - strand at 2346.319 kb on + strand at 2346.319 kb on + strand at 2346.319 kb on + strand at 2346.455 kb on + strand at 2346.527 kb on - strand, within Pf6N2E2_1980 at 2346.557 kb on - strand, within Pf6N2E2_1980 at 2346.559 kb on + strand, within Pf6N2E2_1980 at 2346.559 kb on + strand, within Pf6N2E2_1980 at 2346.560 kb on - strand, within Pf6N2E2_1980 at 2346.617 kb on + strand, within Pf6N2E2_1980 at 2346.634 kb on + strand, within Pf6N2E2_1980 at 2346.732 kb on + strand, within Pf6N2E2_1980 at 2346.735 kb on + strand, within Pf6N2E2_1980 at 2346.736 kb on - strand, within Pf6N2E2_1980 at 2346.736 kb on - strand, within Pf6N2E2_1980 at 2346.738 kb on + strand, within Pf6N2E2_1980 at 2346.738 kb on + strand, within Pf6N2E2_1980 at 2346.738 kb on + strand, within Pf6N2E2_1980 at 2346.738 kb on + strand, within Pf6N2E2_1980 at 2346.738 kb on + strand, within Pf6N2E2_1980 at 2346.739 kb on - strand, within Pf6N2E2_1980 at 2346.828 kb on + strand, within Pf6N2E2_1980 at 2346.850 kb on + strand at 2346.850 kb on + strand at 2346.872 kb on + strand at 2346.873 kb on - strand at 2346.884 kb on + strand at 2347.036 kb on + strand at 2347.066 kb on + strand, within Pf6N2E2_1981 at 2347.115 kb on + strand, within Pf6N2E2_1981 at 2347.115 kb on + strand, within Pf6N2E2_1981 at 2347.116 kb on - strand, within Pf6N2E2_1981 at 2347.205 kb on + strand, within Pf6N2E2_1981
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 2,344,124 + Pf6N2E2_1978 0.17 -2.4 2,344,152 + Pf6N2E2_1978 0.23 -2.3 2,344,153 - Pf6N2E2_1978 0.23 +1.6 2,344,153 - Pf6N2E2_1978 0.23 -1.9 2,344,158 + Pf6N2E2_1978 0.24 -2.7 2,344,159 - Pf6N2E2_1978 0.24 -0.0 2,344,159 - Pf6N2E2_1978 0.24 +1.3 2,344,159 - Pf6N2E2_1978 0.24 +1.1 2,344,159 - Pf6N2E2_1978 0.24 -1.8 2,344,159 - Pf6N2E2_1978 0.24 -1.9 2,344,659 - -2.3 2,344,659 - -1.8 2,344,749 + +0.7 2,344,749 + +1.2 2,344,749 + -1.4 2,344,750 - -0.1 2,344,750 - +1.4 2,344,750 - -3.5 2,344,750 - -2.1 2,345,048 + +2.6 2,345,101 - +1.4 2,345,130 + -2.3 2,345,131 - -1.4 2,345,175 + Pf6N2E2_1979 0.12 -0.4 2,345,176 - Pf6N2E2_1979 0.12 -0.6 2,345,180 - Pf6N2E2_1979 0.13 -0.1 2,345,180 - Pf6N2E2_1979 0.13 +1.4 2,345,650 - Pf6N2E2_1979 0.50 -1.4 2,345,701 - Pf6N2E2_1979 0.54 -0.4 2,345,745 + Pf6N2E2_1979 0.58 -1.8 2,345,745 + Pf6N2E2_1979 0.58 +1.3 2,345,745 + Pf6N2E2_1979 0.58 -1.6 2,345,746 - Pf6N2E2_1979 0.58 -0.0 2,345,746 - Pf6N2E2_1979 0.58 -0.2 2,345,746 - Pf6N2E2_1979 0.58 -0.8 2,345,746 - Pf6N2E2_1979 0.58 +2.1 2,345,919 + Pf6N2E2_1979 0.71 +0.6 2,345,920 - Pf6N2E2_1979 0.72 -1.9 2,345,920 - Pf6N2E2_1979 0.72 +1.1 2,346,023 + Pf6N2E2_1979 0.80 -2.8 2,346,023 + Pf6N2E2_1979 0.80 -1.6 2,346,024 - Pf6N2E2_1979 0.80 -0.2 2,346,024 - Pf6N2E2_1979 0.80 -0.9 2,346,024 - Pf6N2E2_1979 0.80 -3.1 2,346,024 - Pf6N2E2_1979 0.80 +2.0 2,346,150 + Pf6N2E2_1979 0.90 +0.8 2,346,150 + Pf6N2E2_1979 0.90 +0.7 2,346,211 + -1.9 2,346,237 + -1.3 2,346,237 + +1.8 2,346,237 + +1.7 2,346,238 - -3.8 2,346,319 + -0.6 2,346,319 + +1.0 2,346,319 + -0.4 2,346,455 + +0.6 2,346,527 - Pf6N2E2_1980 0.11 -2.4 2,346,557 - Pf6N2E2_1980 0.19 -2.4 2,346,559 + Pf6N2E2_1980 0.19 -2.2 2,346,559 + Pf6N2E2_1980 0.19 +1.1 2,346,560 - Pf6N2E2_1980 0.19 +0.0 2,346,617 + Pf6N2E2_1980 0.33 +2.4 2,346,634 + Pf6N2E2_1980 0.38 -1.9 2,346,732 + Pf6N2E2_1980 0.62 +1.3 2,346,735 + Pf6N2E2_1980 0.62 +0.4 2,346,736 - Pf6N2E2_1980 0.63 -0.0 2,346,736 - Pf6N2E2_1980 0.63 -2.0 2,346,738 + Pf6N2E2_1980 0.63 +0.8 2,346,738 + Pf6N2E2_1980 0.63 +0.9 2,346,738 + Pf6N2E2_1980 0.63 -3.2 2,346,738 + Pf6N2E2_1980 0.63 +2.2 2,346,738 + Pf6N2E2_1980 0.63 -0.3 2,346,739 - Pf6N2E2_1980 0.63 +1.2 2,346,828 + Pf6N2E2_1980 0.85 -2.7 2,346,850 + -0.5 2,346,850 + -2.1 2,346,872 + +0.5 2,346,873 - -1.4 2,346,884 + -1.6 2,347,036 + -2.1 2,347,066 + Pf6N2E2_1981 0.12 -1.6 2,347,115 + Pf6N2E2_1981 0.22 -1.1 2,347,115 + Pf6N2E2_1981 0.22 +0.5 2,347,116 - Pf6N2E2_1981 0.22 +1.1 2,347,205 + Pf6N2E2_1981 0.41 -2.8
Or see this region's nucleotide sequence