Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1924 and Pf6N2E2_1925 are separated by 52 nucleotides Pf6N2E2_1925 and Pf6N2E2_1926 overlap by 4 nucleotides Pf6N2E2_1926 and Pf6N2E2_1927 are separated by 421 nucleotides
Pf6N2E2_1924 - 6-phosphogluconolactonase (EC 3.1.1.31), at 2,279,013 to 2,280,185
_1924
Pf6N2E2_1925 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 2,280,238 to 2,280,876
_1925
Pf6N2E2_1926 - hypothetical protein, at 2,280,873 to 2,281,016
_1926
Pf6N2E2_1927 - Capsule biosynthesis protein capA, at 2,281,438 to 2,282,553
_1927
Position (kb)
2280
2281 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2279.267 kb on - strand, within Pf6N2E2_1924 at 2279.294 kb on - strand, within Pf6N2E2_1924 at 2279.294 kb on - strand, within Pf6N2E2_1924 at 2279.303 kb on + strand, within Pf6N2E2_1924 at 2279.388 kb on - strand, within Pf6N2E2_1924 at 2279.436 kb on + strand, within Pf6N2E2_1924 at 2279.457 kb on + strand, within Pf6N2E2_1924 at 2279.482 kb on + strand, within Pf6N2E2_1924 at 2279.482 kb on + strand, within Pf6N2E2_1924 at 2279.682 kb on + strand, within Pf6N2E2_1924 at 2279.682 kb on + strand, within Pf6N2E2_1924 at 2279.682 kb on + strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.817 kb on + strand, within Pf6N2E2_1924 at 2279.817 kb on + strand, within Pf6N2E2_1924 at 2279.891 kb on - strand, within Pf6N2E2_1924 at 2279.898 kb on + strand, within Pf6N2E2_1924 at 2279.903 kb on + strand, within Pf6N2E2_1924 at 2280.033 kb on + strand, within Pf6N2E2_1924 at 2280.034 kb on - strand, within Pf6N2E2_1924 at 2280.034 kb on - strand, within Pf6N2E2_1924 at 2280.111 kb on + strand at 2280.111 kb on + strand at 2280.112 kb on - strand at 2280.112 kb on - strand at 2280.167 kb on + strand at 2280.167 kb on + strand at 2280.234 kb on - strand at 2280.239 kb on + strand at 2280.239 kb on + strand at 2280.239 kb on + strand at 2280.239 kb on + strand at 2280.239 kb on + strand at 2280.240 kb on - strand at 2280.244 kb on - strand at 2280.488 kb on + strand, within Pf6N2E2_1925 at 2280.489 kb on - strand, within Pf6N2E2_1925 at 2280.544 kb on - strand, within Pf6N2E2_1925 at 2280.551 kb on + strand, within Pf6N2E2_1925 at 2280.551 kb on + strand, within Pf6N2E2_1925 at 2280.551 kb on + strand, within Pf6N2E2_1925 at 2280.552 kb on - strand, within Pf6N2E2_1925 at 2280.560 kb on + strand, within Pf6N2E2_1925 at 2280.561 kb on - strand, within Pf6N2E2_1925 at 2280.566 kb on + strand, within Pf6N2E2_1925 at 2280.566 kb on + strand, within Pf6N2E2_1925 at 2280.567 kb on - strand, within Pf6N2E2_1925 at 2280.570 kb on + strand, within Pf6N2E2_1925 at 2280.634 kb on + strand, within Pf6N2E2_1925 at 2280.634 kb on + strand, within Pf6N2E2_1925 at 2280.645 kb on + strand, within Pf6N2E2_1925 at 2281.006 kb on - strand at 2281.046 kb on + strand at 2281.135 kb on - strand at 2281.329 kb on + strand at 2281.428 kb on - strand at 2281.457 kb on + strand at 2281.520 kb on - strand at 2281.520 kb on - strand at 2281.585 kb on + strand, within Pf6N2E2_1927 at 2281.585 kb on + strand, within Pf6N2E2_1927 at 2281.702 kb on + strand, within Pf6N2E2_1927 at 2281.703 kb on - strand, within Pf6N2E2_1927 at 2281.703 kb on - strand, within Pf6N2E2_1927 at 2281.703 kb on - strand, within Pf6N2E2_1927 at 2281.840 kb on + strand, within Pf6N2E2_1927 at 2281.840 kb on + strand, within Pf6N2E2_1927 at 2281.841 kb on - strand, within Pf6N2E2_1927 at 2281.841 kb on - strand, within Pf6N2E2_1927 at 2281.843 kb on - strand, within Pf6N2E2_1927 at 2281.843 kb on - strand, within Pf6N2E2_1927 at 2281.851 kb on + strand, within Pf6N2E2_1927
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 2,279,267 - Pf6N2E2_1924 0.22 -2.8 2,279,294 - Pf6N2E2_1924 0.24 +0.5 2,279,294 - Pf6N2E2_1924 0.24 -2.8 2,279,303 + Pf6N2E2_1924 0.25 -1.7 2,279,388 - Pf6N2E2_1924 0.32 -0.0 2,279,436 + Pf6N2E2_1924 0.36 +0.2 2,279,457 + Pf6N2E2_1924 0.38 -0.6 2,279,482 + Pf6N2E2_1924 0.40 -0.5 2,279,482 + Pf6N2E2_1924 0.40 +0.3 2,279,682 + Pf6N2E2_1924 0.57 -0.0 2,279,682 + Pf6N2E2_1924 0.57 -1.0 2,279,682 + Pf6N2E2_1924 0.57 +0.5 2,279,683 - Pf6N2E2_1924 0.57 +1.5 2,279,683 - Pf6N2E2_1924 0.57 +0.8 2,279,683 - Pf6N2E2_1924 0.57 -1.9 2,279,683 - Pf6N2E2_1924 0.57 +0.1 2,279,683 - Pf6N2E2_1924 0.57 -0.5 2,279,683 - Pf6N2E2_1924 0.57 +2.8 2,279,817 + Pf6N2E2_1924 0.69 +1.2 2,279,817 + Pf6N2E2_1924 0.69 +1.8 2,279,891 - Pf6N2E2_1924 0.75 -0.8 2,279,898 + Pf6N2E2_1924 0.75 +0.8 2,279,903 + Pf6N2E2_1924 0.76 -0.4 2,280,033 + Pf6N2E2_1924 0.87 +2.5 2,280,034 - Pf6N2E2_1924 0.87 -0.0 2,280,034 - Pf6N2E2_1924 0.87 -1.5 2,280,111 + +1.8 2,280,111 + +0.4 2,280,112 - +2.0 2,280,112 - -1.1 2,280,167 + +0.9 2,280,167 + -2.5 2,280,234 - +0.1 2,280,239 + -2.5 2,280,239 + +0.9 2,280,239 + -1.2 2,280,239 + -2.0 2,280,239 + +1.5 2,280,240 - -2.8 2,280,244 - +0.2 2,280,488 + Pf6N2E2_1925 0.39 +3.2 2,280,489 - Pf6N2E2_1925 0.39 +0.4 2,280,544 - Pf6N2E2_1925 0.48 +0.5 2,280,551 + Pf6N2E2_1925 0.49 +1.4 2,280,551 + Pf6N2E2_1925 0.49 +1.4 2,280,551 + Pf6N2E2_1925 0.49 +2.8 2,280,552 - Pf6N2E2_1925 0.49 +1.1 2,280,560 + Pf6N2E2_1925 0.50 -2.7 2,280,561 - Pf6N2E2_1925 0.51 +2.0 2,280,566 + Pf6N2E2_1925 0.51 -0.6 2,280,566 + Pf6N2E2_1925 0.51 -1.3 2,280,567 - Pf6N2E2_1925 0.51 -1.3 2,280,570 + Pf6N2E2_1925 0.52 -3.0 2,280,634 + Pf6N2E2_1925 0.62 +0.7 2,280,634 + Pf6N2E2_1925 0.62 +2.6 2,280,645 + Pf6N2E2_1925 0.64 +0.5 2,281,006 - +2.4 2,281,046 + +1.0 2,281,135 - -2.8 2,281,329 + +1.0 2,281,428 - -0.5 2,281,457 + -2.5 2,281,520 - -2.8 2,281,520 - +2.3 2,281,585 + Pf6N2E2_1927 0.13 -0.0 2,281,585 + Pf6N2E2_1927 0.13 +1.5 2,281,702 + Pf6N2E2_1927 0.24 +0.5 2,281,703 - Pf6N2E2_1927 0.24 -1.5 2,281,703 - Pf6N2E2_1927 0.24 +2.9 2,281,703 - Pf6N2E2_1927 0.24 +0.5 2,281,840 + Pf6N2E2_1927 0.36 -0.8 2,281,840 + Pf6N2E2_1927 0.36 -3.2 2,281,841 - Pf6N2E2_1927 0.36 +1.0 2,281,841 - Pf6N2E2_1927 0.36 -0.4 2,281,843 - Pf6N2E2_1927 0.36 +1.1 2,281,843 - Pf6N2E2_1927 0.36 -3.0 2,281,851 + Pf6N2E2_1927 0.37 -0.8
Or see this region's nucleotide sequence