Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_185 and Pf6N2E2_186 overlap by 4 nucleotides Pf6N2E2_186 and Pf6N2E2_187 are separated by 10 nucleotides Pf6N2E2_187 and Pf6N2E2_188 are separated by 132 nucleotides
Pf6N2E2_185 - Urea ABC transporter, permease protein UrtC, at 237,965 to 239,152
_185
Pf6N2E2_186 - Urea ABC transporter, ATPase protein UrtD, at 239,149 to 239,907
_186
Pf6N2E2_187 - Urea ABC transporter, ATPase protein UrtE, at 239,918 to 240,607
_187
Pf6N2E2_188 - Formamidase (EC 3.5.1.49), at 240,740 to 241,969
_188
Position (kb) 239
240
241 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 238.944 kb on - strand, within Pf6N2E2_185 at 238.979 kb on + strand, within Pf6N2E2_185 at 238.980 kb on - strand, within Pf6N2E2_185 at 239.248 kb on + strand, within Pf6N2E2_186 at 239.248 kb on + strand, within Pf6N2E2_186 at 239.248 kb on + strand, within Pf6N2E2_186 at 239.249 kb on - strand, within Pf6N2E2_186 at 239.249 kb on - strand, within Pf6N2E2_186 at 239.249 kb on - strand, within Pf6N2E2_186 at 239.393 kb on - strand, within Pf6N2E2_186 at 239.393 kb on - strand, within Pf6N2E2_186 at 239.717 kb on + strand, within Pf6N2E2_186 at 239.808 kb on + strand, within Pf6N2E2_186 at 239.893 kb on + strand at 239.893 kb on + strand at 239.894 kb on - strand at 239.897 kb on + strand at 239.928 kb on + strand at 239.928 kb on + strand at 239.929 kb on - strand at 240.155 kb on + strand, within Pf6N2E2_187 at 240.155 kb on + strand, within Pf6N2E2_187 at 240.156 kb on - strand, within Pf6N2E2_187 at 240.156 kb on - strand, within Pf6N2E2_187 at 240.185 kb on - strand, within Pf6N2E2_187 at 240.207 kb on + strand, within Pf6N2E2_187 at 240.207 kb on + strand, within Pf6N2E2_187 at 240.207 kb on + strand, within Pf6N2E2_187 at 240.257 kb on + strand, within Pf6N2E2_187 at 240.606 kb on - strand at 240.626 kb on + strand at 240.626 kb on + strand at 240.626 kb on + strand at 240.627 kb on - strand at 240.627 kb on - strand at 240.627 kb on - strand at 240.627 kb on - strand at 240.636 kb on + strand at 240.654 kb on + strand at 240.654 kb on + strand at 240.669 kb on - strand at 240.712 kb on + strand at 240.712 kb on + strand at 240.859 kb on + strand at 240.859 kb on + strand at 240.873 kb on - strand, within Pf6N2E2_188 at 240.873 kb on - strand, within Pf6N2E2_188 at 240.873 kb on - strand, within Pf6N2E2_188 at 240.950 kb on + strand, within Pf6N2E2_188 at 240.950 kb on + strand, within Pf6N2E2_188 at 240.951 kb on - strand, within Pf6N2E2_188 at 241.405 kb on + strand, within Pf6N2E2_188 at 241.406 kb on - strand, within Pf6N2E2_188 at 241.457 kb on + strand, within Pf6N2E2_188 at 241.558 kb on + strand, within Pf6N2E2_188
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 238,944 - Pf6N2E2_185 0.82 +1.2 238,979 + Pf6N2E2_185 0.85 -1.7 238,980 - Pf6N2E2_185 0.85 -0.3 239,248 + Pf6N2E2_186 0.13 -1.4 239,248 + Pf6N2E2_186 0.13 -0.6 239,248 + Pf6N2E2_186 0.13 +1.6 239,249 - Pf6N2E2_186 0.13 +0.2 239,249 - Pf6N2E2_186 0.13 -3.0 239,249 - Pf6N2E2_186 0.13 -1.8 239,393 - Pf6N2E2_186 0.32 -1.3 239,393 - Pf6N2E2_186 0.32 +1.3 239,717 + Pf6N2E2_186 0.75 +1.0 239,808 + Pf6N2E2_186 0.87 -1.4 239,893 + -1.6 239,893 + +2.0 239,894 - -0.9 239,897 + +2.5 239,928 + -1.5 239,928 + +1.1 239,929 - -1.1 240,155 + Pf6N2E2_187 0.34 -2.5 240,155 + Pf6N2E2_187 0.34 -3.2 240,156 - Pf6N2E2_187 0.34 +2.2 240,156 - Pf6N2E2_187 0.34 -0.6 240,185 - Pf6N2E2_187 0.39 -1.7 240,207 + Pf6N2E2_187 0.42 +1.9 240,207 + Pf6N2E2_187 0.42 +2.0 240,207 + Pf6N2E2_187 0.42 +1.3 240,257 + Pf6N2E2_187 0.49 +0.6 240,606 - +1.9 240,626 + -0.1 240,626 + -2.1 240,626 + -0.6 240,627 - -1.4 240,627 - +0.9 240,627 - +1.5 240,627 - +2.3 240,636 + -2.3 240,654 + -1.5 240,654 + -0.5 240,669 - +1.6 240,712 + +0.1 240,712 + -2.7 240,859 + -2.3 240,859 + +0.7 240,873 - Pf6N2E2_188 0.11 -2.7 240,873 - Pf6N2E2_188 0.11 +1.0 240,873 - Pf6N2E2_188 0.11 -1.2 240,950 + Pf6N2E2_188 0.17 -1.3 240,950 + Pf6N2E2_188 0.17 +2.1 240,951 - Pf6N2E2_188 0.17 -1.3 241,405 + Pf6N2E2_188 0.54 -0.3 241,406 - Pf6N2E2_188 0.54 +0.1 241,457 + Pf6N2E2_188 0.58 -0.3 241,558 + Pf6N2E2_188 0.67 -1.4
Or see this region's nucleotide sequence