Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_181

Experiment: deoxycholate 0.0125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_179 and Pf6N2E2_180 overlap by 20 nucleotidesPf6N2E2_180 and Pf6N2E2_6104 are separated by 90 nucleotidesPf6N2E2_6104 and Pf6N2E2_181 are separated by 193 nucleotidesPf6N2E2_181 and Pf6N2E2_182 overlap by 26 nucleotides Pf6N2E2_179 - Integration host factor alpha subunit, at 230,305 to 230,607 _179 Pf6N2E2_180 - Transcriptional regulator, MerR family, at 230,588 to 230,944 _180 Pf6N2E2_6104 - tRNA-Pro-GGG, at 231,035 to 231,108 _6104 Pf6N2E2_181 - Two-component response regulator, at 231,302 to 231,994 _181 Pf6N2E2_182 - Two-component hybrid sensor and regulator, at 231,969 to 235,361 _182 Position (kb) 231 232Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 230.304 kb on + strandat 230.304 kb on + strandat 230.304 kb on + strandat 230.305 kb on - strandat 230.587 kb on + strandat 230.587 kb on + strandat 230.587 kb on + strandat 230.587 kb on + strandat 230.587 kb on + strandat 230.588 kb on - strandat 230.588 kb on - strandat 230.605 kb on + strandat 230.756 kb on + strand, within Pf6N2E2_180at 230.756 kb on + strand, within Pf6N2E2_180at 230.756 kb on + strand, within Pf6N2E2_180at 230.804 kb on + strand, within Pf6N2E2_180at 230.869 kb on + strand, within Pf6N2E2_180at 230.870 kb on - strand, within Pf6N2E2_180at 231.128 kb on - strandat 231.218 kb on - strandat 231.283 kb on + strandat 231.283 kb on + strandat 231.283 kb on + strandat 231.283 kb on + strandat 231.283 kb on + strandat 231.283 kb on + strandat 231.284 kb on - strandat 231.374 kb on + strand, within Pf6N2E2_181at 231.374 kb on + strand, within Pf6N2E2_181at 231.375 kb on - strand, within Pf6N2E2_181at 231.375 kb on - strand, within Pf6N2E2_181at 231.375 kb on - strand, within Pf6N2E2_181at 231.380 kb on + strand, within Pf6N2E2_181at 231.380 kb on + strand, within Pf6N2E2_181at 231.380 kb on + strand, within Pf6N2E2_181at 231.381 kb on - strand, within Pf6N2E2_181at 231.381 kb on - strand, within Pf6N2E2_181at 231.390 kb on + strand, within Pf6N2E2_181at 231.390 kb on + strand, within Pf6N2E2_181at 231.390 kb on + strand, within Pf6N2E2_181at 231.390 kb on + strand, within Pf6N2E2_181at 231.390 kb on + strand, within Pf6N2E2_181at 231.390 kb on + strand, within Pf6N2E2_181at 231.390 kb on + strand, within Pf6N2E2_181at 231.390 kb on + strand, within Pf6N2E2_181at 231.390 kb on + strand, within Pf6N2E2_181at 231.390 kb on + strand, within Pf6N2E2_181at 231.391 kb on - strand, within Pf6N2E2_181at 231.391 kb on - strand, within Pf6N2E2_181at 231.391 kb on - strand, within Pf6N2E2_181at 231.391 kb on - strand, within Pf6N2E2_181at 231.391 kb on - strand, within Pf6N2E2_181at 231.391 kb on - strand, within Pf6N2E2_181at 231.391 kb on - strand, within Pf6N2E2_181at 231.391 kb on - strand, within Pf6N2E2_181at 231.391 kb on - strand, within Pf6N2E2_181at 231.391 kb on - strand, within Pf6N2E2_181at 231.667 kb on - strand, within Pf6N2E2_181at 231.667 kb on - strand, within Pf6N2E2_181at 231.891 kb on + strand, within Pf6N2E2_181at 231.892 kb on - strand, within Pf6N2E2_181at 231.892 kb on - strand, within Pf6N2E2_181at 232.133 kb on - strandat 232.284 kb on + strandat 232.285 kb on - strandat 232.300 kb on + strandat 232.535 kb on + strand, within Pf6N2E2_182at 232.582 kb on - strand, within Pf6N2E2_182at 232.656 kb on + strand, within Pf6N2E2_182at 232.656 kb on + strand, within Pf6N2E2_182at 232.853 kb on + strand, within Pf6N2E2_182at 232.854 kb on - strand, within Pf6N2E2_182at 232.854 kb on - strand, within Pf6N2E2_182at 232.892 kb on + strand, within Pf6N2E2_182at 232.892 kb on + strand, within Pf6N2E2_182at 232.892 kb on + strand, within Pf6N2E2_182at 232.892 kb on + strand, within Pf6N2E2_182at 232.892 kb on + strand, within Pf6N2E2_182at 232.892 kb on + strand, within Pf6N2E2_182at 232.893 kb on - strand, within Pf6N2E2_182at 232.894 kb on + strand, within Pf6N2E2_182at 232.894 kb on + strand, within Pf6N2E2_182at 232.894 kb on + strand, within Pf6N2E2_182at 232.894 kb on + strand, within Pf6N2E2_182at 232.894 kb on + strand, within Pf6N2E2_182at 232.894 kb on + strand, within Pf6N2E2_182at 232.895 kb on - strand, within Pf6N2E2_182at 232.895 kb on - strand, within Pf6N2E2_182at 232.895 kb on - strand, within Pf6N2E2_182at 232.895 kb on - strand, within Pf6N2E2_182at 232.895 kb on - strand, within Pf6N2E2_182at 232.895 kb on - strand, within Pf6N2E2_182at 232.895 kb on - strand, within Pf6N2E2_182at 232.895 kb on - strand, within Pf6N2E2_182

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Per-strain Table

Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M
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230,304 + -1.4
230,304 + -0.6
230,304 + -2.4
230,305 - -1.1
230,587 + -0.1
230,587 + +1.2
230,587 + -0.1
230,587 + -0.4
230,587 + -1.0
230,588 - +0.1
230,588 - +2.7
230,605 + -0.9
230,756 + Pf6N2E2_180 0.47 -0.1
230,756 + Pf6N2E2_180 0.47 +0.7
230,756 + Pf6N2E2_180 0.47 +0.6
230,804 + Pf6N2E2_180 0.61 +2.3
230,869 + Pf6N2E2_180 0.79 +0.8
230,870 - Pf6N2E2_180 0.79 -2.1
231,128 - -1.2
231,218 - -1.3
231,283 + +1.1
231,283 + +3.2
231,283 + -0.3
231,283 + -0.4
231,283 + -2.6
231,283 + +1.1
231,284 - -1.6
231,374 + Pf6N2E2_181 0.10 +0.7
231,374 + Pf6N2E2_181 0.10 -0.9
231,375 - Pf6N2E2_181 0.11 -2.2
231,375 - Pf6N2E2_181 0.11 +0.7
231,375 - Pf6N2E2_181 0.11 +0.6
231,380 + Pf6N2E2_181 0.11 +1.6
231,380 + Pf6N2E2_181 0.11 -2.7
231,380 + Pf6N2E2_181 0.11 -1.1
231,381 - Pf6N2E2_181 0.11 -3.0
231,381 - Pf6N2E2_181 0.11 +1.4
231,390 + Pf6N2E2_181 0.13 -2.6
231,390 + Pf6N2E2_181 0.13 +0.4
231,390 + Pf6N2E2_181 0.13 +0.8
231,390 + Pf6N2E2_181 0.13 -0.5
231,390 + Pf6N2E2_181 0.13 -0.7
231,390 + Pf6N2E2_181 0.13 -1.1
231,390 + Pf6N2E2_181 0.13 +0.6
231,390 + Pf6N2E2_181 0.13 +1.8
231,390 + Pf6N2E2_181 0.13 -0.1
231,390 + Pf6N2E2_181 0.13 -2.1
231,391 - Pf6N2E2_181 0.13 +0.1
231,391 - Pf6N2E2_181 0.13 -2.9
231,391 - Pf6N2E2_181 0.13 -1.4
231,391 - Pf6N2E2_181 0.13 -0.3
231,391 - Pf6N2E2_181 0.13 -2.7
231,391 - Pf6N2E2_181 0.13 +0.6
231,391 - Pf6N2E2_181 0.13 -1.2
231,391 - Pf6N2E2_181 0.13 +0.1
231,391 - Pf6N2E2_181 0.13 +1.7
231,391 - Pf6N2E2_181 0.13 +0.1
231,667 - Pf6N2E2_181 0.53 +1.7
231,667 - Pf6N2E2_181 0.53 -2.3
231,891 + Pf6N2E2_181 0.85 -0.7
231,892 - Pf6N2E2_181 0.85 -3.1
231,892 - Pf6N2E2_181 0.85 +0.0
232,133 - -2.3
232,284 + -3.0
232,285 - +1.7
232,300 + +1.2
232,535 + Pf6N2E2_182 0.17 +1.9
232,582 - Pf6N2E2_182 0.18 +2.2
232,656 + Pf6N2E2_182 0.20 +1.4
232,656 + Pf6N2E2_182 0.20 +1.4
232,853 + Pf6N2E2_182 0.26 -0.3
232,854 - Pf6N2E2_182 0.26 +1.4
232,854 - Pf6N2E2_182 0.26 +2.4
232,892 + Pf6N2E2_182 0.27 +0.8
232,892 + Pf6N2E2_182 0.27 +3.8
232,892 + Pf6N2E2_182 0.27 +0.5
232,892 + Pf6N2E2_182 0.27 -0.3
232,892 + Pf6N2E2_182 0.27 -0.1
232,892 + Pf6N2E2_182 0.27 -1.4
232,893 - Pf6N2E2_182 0.27 -2.2
232,894 + Pf6N2E2_182 0.27 -1.9
232,894 + Pf6N2E2_182 0.27 -0.1
232,894 + Pf6N2E2_182 0.27 -3.7
232,894 + Pf6N2E2_182 0.27 +0.1
232,894 + Pf6N2E2_182 0.27 -0.6
232,894 + Pf6N2E2_182 0.27 -2.4
232,895 - Pf6N2E2_182 0.27 +0.3
232,895 - Pf6N2E2_182 0.27 -1.9
232,895 - Pf6N2E2_182 0.27 -0.7
232,895 - Pf6N2E2_182 0.27 -0.7
232,895 - Pf6N2E2_182 0.27 +0.8
232,895 - Pf6N2E2_182 0.27 +0.1
232,895 - Pf6N2E2_182 0.27 +1.0
232,895 - Pf6N2E2_182 0.27 +1.0

Or see this region's nucleotide sequence