Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1661 and Pf6N2E2_1662 are separated by 132 nucleotides Pf6N2E2_1662 and Pf6N2E2_1663 are separated by 31 nucleotides Pf6N2E2_1663 and Pf6N2E2_1664 overlap by 4 nucleotides
Pf6N2E2_1661 - RND efflux system, inner membrane transporter CmeB, at 1,965,742 to 1,968,837
_1661
Pf6N2E2_1662 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase., at 1,968,970 to 1,969,818
_1662
Pf6N2E2_1663 - 2-keto-3-deoxy-L-fuconate dehydrogenase, at 1,969,850 to 1,970,590
_1663
Pf6N2E2_1664 - Nitrate/nitrite transporter, at 1,970,587 to 1,971,969
_1664
Position (kb)
1968
1969
1970 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1968.038 kb on + strand, within Pf6N2E2_1661 at 1968.178 kb on + strand, within Pf6N2E2_1661 at 1968.178 kb on + strand, within Pf6N2E2_1661 at 1968.178 kb on + strand, within Pf6N2E2_1661 at 1968.398 kb on - strand, within Pf6N2E2_1661 at 1968.461 kb on + strand, within Pf6N2E2_1661 at 1969.222 kb on + strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.409 kb on + strand, within Pf6N2E2_1662 at 1969.410 kb on - strand, within Pf6N2E2_1662 at 1969.410 kb on - strand, within Pf6N2E2_1662 at 1969.521 kb on + strand, within Pf6N2E2_1662 at 1969.522 kb on - strand, within Pf6N2E2_1662 at 1969.810 kb on + strand at 1969.810 kb on + strand at 1969.855 kb on + strand at 1969.921 kb on - strand at 1969.921 kb on - strand at 1969.993 kb on - strand, within Pf6N2E2_1663 at 1970.274 kb on + strand, within Pf6N2E2_1663 at 1970.274 kb on + strand, within Pf6N2E2_1663 at 1970.333 kb on + strand, within Pf6N2E2_1663 at 1970.333 kb on + strand, within Pf6N2E2_1663 at 1970.333 kb on + strand, within Pf6N2E2_1663 at 1970.334 kb on - strand, within Pf6N2E2_1663 at 1970.334 kb on - strand, within Pf6N2E2_1663 at 1970.334 kb on - strand, within Pf6N2E2_1663 at 1970.353 kb on - strand, within Pf6N2E2_1663 at 1970.353 kb on - strand, within Pf6N2E2_1663 at 1970.567 kb on + strand at 1970.582 kb on + strand at 1970.583 kb on - strand at 1970.699 kb on - strand at 1970.699 kb on - strand at 1970.699 kb on - strand at 1970.699 kb on - strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.703 kb on - strand at 1970.703 kb on - strand at 1970.703 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 1,968,038 + Pf6N2E2_1661 0.74 -1.9 1,968,178 + Pf6N2E2_1661 0.79 -3.2 1,968,178 + Pf6N2E2_1661 0.79 +0.1 1,968,178 + Pf6N2E2_1661 0.79 -1.5 1,968,398 - Pf6N2E2_1661 0.86 -0.1 1,968,461 + Pf6N2E2_1661 0.88 -1.7 1,969,222 + Pf6N2E2_1662 0.30 -0.5 1,969,223 - Pf6N2E2_1662 0.30 +1.6 1,969,223 - Pf6N2E2_1662 0.30 +0.7 1,969,223 - Pf6N2E2_1662 0.30 -3.1 1,969,409 + Pf6N2E2_1662 0.52 -1.0 1,969,410 - Pf6N2E2_1662 0.52 -1.7 1,969,410 - Pf6N2E2_1662 0.52 -0.9 1,969,521 + Pf6N2E2_1662 0.65 -0.7 1,969,522 - Pf6N2E2_1662 0.65 -1.1 1,969,810 + -2.1 1,969,810 + +1.6 1,969,855 + +2.3 1,969,921 - +2.5 1,969,921 - -3.1 1,969,993 - Pf6N2E2_1663 0.19 +3.3 1,970,274 + Pf6N2E2_1663 0.57 -0.4 1,970,274 + Pf6N2E2_1663 0.57 -0.9 1,970,333 + Pf6N2E2_1663 0.65 -0.8 1,970,333 + Pf6N2E2_1663 0.65 +1.9 1,970,333 + Pf6N2E2_1663 0.65 +1.3 1,970,334 - Pf6N2E2_1663 0.65 -2.0 1,970,334 - Pf6N2E2_1663 0.65 -1.3 1,970,334 - Pf6N2E2_1663 0.65 -0.8 1,970,353 - Pf6N2E2_1663 0.68 -1.5 1,970,353 - Pf6N2E2_1663 0.68 +3.0 1,970,567 + -1.9 1,970,582 + -0.5 1,970,583 - -0.0 1,970,699 - +1.0 1,970,699 - -1.1 1,970,699 - -1.7 1,970,699 - +1.1 1,970,702 + +0.8 1,970,702 + -1.9 1,970,702 + -0.8 1,970,702 + -1.9 1,970,702 + -0.9 1,970,702 + +1.5 1,970,702 + -1.5 1,970,702 + -1.5 1,970,702 + +1.3 1,970,703 - -1.9 1,970,703 - +1.2 1,970,703 - +0.4
Or see this region's nucleotide sequence