Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_148 and Pf6N2E2_149 are separated by 274 nucleotides Pf6N2E2_149 and Pf6N2E2_150 are separated by 155 nucleotides Pf6N2E2_150 and Pf6N2E2_151 are separated by 2 nucleotides
Pf6N2E2_148 - Membrane fusion component of tripartite multidrug resistance system, at 203,029 to 204,225
_148
Pf6N2E2_149 - Inner membrane component of tripartite multidrug resistance system, at 204,500 to 206,029
_149
Pf6N2E2_150 - UDP-2,3-diacylglucosamine diphosphatase (EC 3.6.1.54), at 206,185 to 206,934
_150
Pf6N2E2_151 - Peptidyl-prolyl cis-trans isomerase PpiB (EC 5.2.1.8), at 206,937 to 207,440
_151
Position (kb) 204
205
206
207 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 203.676 kb on + strand, within Pf6N2E2_148 at 203.677 kb on - strand, within Pf6N2E2_148 at 203.702 kb on - strand, within Pf6N2E2_148 at 203.863 kb on + strand, within Pf6N2E2_148 at 203.864 kb on - strand, within Pf6N2E2_148 at 203.864 kb on - strand, within Pf6N2E2_148 at 203.881 kb on + strand, within Pf6N2E2_148 at 203.881 kb on + strand, within Pf6N2E2_148 at 203.881 kb on + strand, within Pf6N2E2_148 at 203.882 kb on - strand, within Pf6N2E2_148 at 203.936 kb on + strand, within Pf6N2E2_148 at 204.137 kb on - strand at 204.137 kb on - strand at 204.137 kb on - strand at 204.703 kb on + strand, within Pf6N2E2_149 at 204.790 kb on + strand, within Pf6N2E2_149 at 204.790 kb on + strand, within Pf6N2E2_149 at 204.791 kb on - strand, within Pf6N2E2_149 at 204.828 kb on + strand, within Pf6N2E2_149 at 204.829 kb on - strand, within Pf6N2E2_149 at 204.992 kb on + strand, within Pf6N2E2_149 at 204.992 kb on + strand, within Pf6N2E2_149 at 204.992 kb on + strand, within Pf6N2E2_149 at 204.992 kb on + strand, within Pf6N2E2_149 at 204.992 kb on + strand, within Pf6N2E2_149 at 204.992 kb on + strand, within Pf6N2E2_149 at 204.993 kb on - strand, within Pf6N2E2_149 at 204.993 kb on - strand, within Pf6N2E2_149 at 204.993 kb on - strand, within Pf6N2E2_149 at 205.107 kb on - strand, within Pf6N2E2_149 at 205.107 kb on - strand, within Pf6N2E2_149 at 205.340 kb on + strand, within Pf6N2E2_149 at 205.341 kb on - strand, within Pf6N2E2_149 at 205.478 kb on + strand, within Pf6N2E2_149 at 205.479 kb on - strand, within Pf6N2E2_149 at 205.479 kb on - strand, within Pf6N2E2_149 at 205.479 kb on - strand, within Pf6N2E2_149 at 205.787 kb on + strand, within Pf6N2E2_149 at 205.787 kb on + strand, within Pf6N2E2_149 at 205.788 kb on - strand, within Pf6N2E2_149 at 205.788 kb on - strand, within Pf6N2E2_149 at 205.788 kb on - strand, within Pf6N2E2_149 at 205.904 kb on + strand at 205.904 kb on + strand at 205.904 kb on + strand at 205.904 kb on + strand at 205.904 kb on + strand at 205.904 kb on + strand at 205.904 kb on + strand at 205.904 kb on + strand at 205.904 kb on + strand at 205.904 kb on + strand at 205.904 kb on + strand at 205.904 kb on + strand at 205.904 kb on + strand at 205.905 kb on - strand at 205.905 kb on - strand at 205.905 kb on - strand at 205.905 kb on - strand at 205.905 kb on - strand at 205.905 kb on - strand at 205.905 kb on - strand at 205.905 kb on - strand at 205.905 kb on - strand at 205.905 kb on - strand at 205.905 kb on - strand at 205.905 kb on - strand at 205.925 kb on + strand at 205.925 kb on + strand at 205.926 kb on - strand at 205.926 kb on - strand at 205.926 kb on - strand at 205.926 kb on - strand at 205.926 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 203,676 + Pf6N2E2_148 0.54 -1.4 203,677 - Pf6N2E2_148 0.54 +0.0 203,702 - Pf6N2E2_148 0.56 +0.9 203,863 + Pf6N2E2_148 0.70 -1.5 203,864 - Pf6N2E2_148 0.70 -0.6 203,864 - Pf6N2E2_148 0.70 -1.7 203,881 + Pf6N2E2_148 0.71 +0.2 203,881 + Pf6N2E2_148 0.71 +1.9 203,881 + Pf6N2E2_148 0.71 -1.1 203,882 - Pf6N2E2_148 0.71 +0.7 203,936 + Pf6N2E2_148 0.76 +1.1 204,137 - -0.2 204,137 - +1.2 204,137 - -2.3 204,703 + Pf6N2E2_149 0.13 +0.7 204,790 + Pf6N2E2_149 0.19 -3.5 204,790 + Pf6N2E2_149 0.19 -0.5 204,791 - Pf6N2E2_149 0.19 +1.7 204,828 + Pf6N2E2_149 0.21 +1.2 204,829 - Pf6N2E2_149 0.22 -1.3 204,992 + Pf6N2E2_149 0.32 -0.8 204,992 + Pf6N2E2_149 0.32 -1.6 204,992 + Pf6N2E2_149 0.32 -1.7 204,992 + Pf6N2E2_149 0.32 +1.0 204,992 + Pf6N2E2_149 0.32 -1.6 204,992 + Pf6N2E2_149 0.32 -2.7 204,993 - Pf6N2E2_149 0.32 -2.2 204,993 - Pf6N2E2_149 0.32 +2.0 204,993 - Pf6N2E2_149 0.32 +1.6 205,107 - Pf6N2E2_149 0.40 -0.8 205,107 - Pf6N2E2_149 0.40 -0.7 205,340 + Pf6N2E2_149 0.55 -3.7 205,341 - Pf6N2E2_149 0.55 -0.6 205,478 + Pf6N2E2_149 0.64 -3.7 205,479 - Pf6N2E2_149 0.64 -1.4 205,479 - Pf6N2E2_149 0.64 -3.1 205,479 - Pf6N2E2_149 0.64 +0.7 205,787 + Pf6N2E2_149 0.84 +1.3 205,787 + Pf6N2E2_149 0.84 -0.6 205,788 - Pf6N2E2_149 0.84 +1.4 205,788 - Pf6N2E2_149 0.84 -2.9 205,788 - Pf6N2E2_149 0.84 -1.4 205,904 + +0.9 205,904 + -0.1 205,904 + -2.6 205,904 + -0.5 205,904 + -2.6 205,904 + +0.5 205,904 + +0.5 205,904 + +0.5 205,904 + +1.7 205,904 + -2.0 205,904 + -2.1 205,904 + -0.6 205,904 + -0.3 205,905 - -2.5 205,905 - +0.5 205,905 - +0.6 205,905 - -0.9 205,905 - +0.6 205,905 - +1.4 205,905 - -1.4 205,905 - -1.5 205,905 - -0.3 205,905 - +0.3 205,905 - +1.1 205,905 - -0.8 205,925 + -1.1 205,925 + -0.6 205,926 - -1.1 205,926 - +0.5 205,926 - +1.0 205,926 - +0.0 205,926 - +0.6
Or see this region's nucleotide sequence