Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_136 and Pf6N2E2_137 overlap by 1 nucleotides Pf6N2E2_137 and Pf6N2E2_138 are separated by 30 nucleotides
Pf6N2E2_136 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 185,041 to 186,099
_136
Pf6N2E2_137 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 186,099 to 187,634
_137
Pf6N2E2_138 - Alpha/beta hydrolase fold (EC 3.8.1.5), at 187,665 to 188,594
_138
Position (kb) 186
187
188 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 185.114 kb on + strand at 185.376 kb on - strand, within Pf6N2E2_136 at 185.557 kb on - strand, within Pf6N2E2_136 at 185.557 kb on - strand, within Pf6N2E2_136 at 185.928 kb on - strand, within Pf6N2E2_136 at 186.545 kb on - strand, within Pf6N2E2_137 at 186.547 kb on + strand, within Pf6N2E2_137 at 186.547 kb on + strand, within Pf6N2E2_137 at 186.547 kb on + strand, within Pf6N2E2_137 at 186.547 kb on + strand, within Pf6N2E2_137 at 186.548 kb on - strand, within Pf6N2E2_137 at 186.548 kb on - strand, within Pf6N2E2_137 at 186.548 kb on - strand, within Pf6N2E2_137 at 187.085 kb on - strand, within Pf6N2E2_137 at 187.168 kb on + strand, within Pf6N2E2_137 at 187.168 kb on + strand, within Pf6N2E2_137 at 187.168 kb on + strand, within Pf6N2E2_137 at 187.171 kb on + strand, within Pf6N2E2_137 at 187.171 kb on + strand, within Pf6N2E2_137 at 187.172 kb on - strand, within Pf6N2E2_137 at 187.293 kb on + strand, within Pf6N2E2_137 at 187.293 kb on + strand, within Pf6N2E2_137 at 187.294 kb on - strand, within Pf6N2E2_137 at 187.359 kb on + strand, within Pf6N2E2_137 at 187.360 kb on - strand, within Pf6N2E2_137 at 187.468 kb on + strand, within Pf6N2E2_137 at 187.585 kb on + strand at 187.585 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.840 kb on - strand, within Pf6N2E2_138 at 187.840 kb on - strand, within Pf6N2E2_138 at 187.840 kb on - strand, within Pf6N2E2_138 at 188.005 kb on + strand, within Pf6N2E2_138 at 188.006 kb on - strand, within Pf6N2E2_138 at 188.171 kb on - strand, within Pf6N2E2_138 at 188.263 kb on + strand, within Pf6N2E2_138 at 188.326 kb on + strand, within Pf6N2E2_138 at 188.381 kb on - strand, within Pf6N2E2_138 at 188.515 kb on + strand at 188.540 kb on - strand at 188.540 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 185,114 + -2.1 185,376 - Pf6N2E2_136 0.32 +0.9 185,557 - Pf6N2E2_136 0.49 +0.6 185,557 - Pf6N2E2_136 0.49 -0.7 185,928 - Pf6N2E2_136 0.84 -1.1 186,545 - Pf6N2E2_137 0.29 -2.5 186,547 + Pf6N2E2_137 0.29 -2.7 186,547 + Pf6N2E2_137 0.29 -2.2 186,547 + Pf6N2E2_137 0.29 -2.9 186,547 + Pf6N2E2_137 0.29 +0.2 186,548 - Pf6N2E2_137 0.29 +1.8 186,548 - Pf6N2E2_137 0.29 +1.1 186,548 - Pf6N2E2_137 0.29 -1.7 187,085 - Pf6N2E2_137 0.64 -0.6 187,168 + Pf6N2E2_137 0.70 -1.2 187,168 + Pf6N2E2_137 0.70 +2.0 187,168 + Pf6N2E2_137 0.70 +1.8 187,171 + Pf6N2E2_137 0.70 -1.4 187,171 + Pf6N2E2_137 0.70 +1.0 187,172 - Pf6N2E2_137 0.70 -2.2 187,293 + Pf6N2E2_137 0.78 -2.6 187,293 + Pf6N2E2_137 0.78 -1.1 187,294 - Pf6N2E2_137 0.78 +0.1 187,359 + Pf6N2E2_137 0.82 -0.8 187,360 - Pf6N2E2_137 0.82 +0.6 187,468 + Pf6N2E2_137 0.89 -2.2 187,585 + +0.6 187,585 + -1.4 187,634 + +1.8 187,634 + -0.4 187,634 + -3.1 187,634 + +0.5 187,634 + -2.4 187,634 + +0.2 187,634 + -1.8 187,634 + +1.1 187,634 + -2.5 187,634 + +0.5 187,634 + -1.7 187,635 - +0.2 187,635 - -1.6 187,635 - +1.6 187,635 - +1.6 187,635 - +0.1 187,635 - +1.8 187,635 - +1.3 187,840 - Pf6N2E2_138 0.19 +2.6 187,840 - Pf6N2E2_138 0.19 -2.5 187,840 - Pf6N2E2_138 0.19 +0.2 188,005 + Pf6N2E2_138 0.37 -1.7 188,006 - Pf6N2E2_138 0.37 -1.1 188,171 - Pf6N2E2_138 0.54 -1.6 188,263 + Pf6N2E2_138 0.64 +1.6 188,326 + Pf6N2E2_138 0.71 -1.6 188,381 - Pf6N2E2_138 0.77 -1.2 188,515 + +0.5 188,540 - -1.2 188,540 - -0.8
Or see this region's nucleotide sequence