Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1308

Experiment: deoxycholate 0.0125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1306 and Pf6N2E2_1307 are separated by 186 nucleotidesPf6N2E2_1307 and Pf6N2E2_1308 overlap by 4 nucleotidesPf6N2E2_1308 and Pf6N2E2_1309 are separated by 5 nucleotides Pf6N2E2_1306 - Transcriptional activator of 4-hydroxyphenylacetate 3-monooxygenase operon, XylS/AraC family, at 1,540,379 to 1,541,284 _1306 Pf6N2E2_1307 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,541,471 to 1,542,148 _1307 Pf6N2E2_1308 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,542,145 to 1,542,915 _1308 Pf6N2E2_1309 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 1,542,921 to 1,544,381 _1309 Position (kb) 1542 1543Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1541.206 kb on - strandat 1541.206 kb on - strandat 1541.390 kb on + strandat 1541.407 kb on - strandat 1541.751 kb on - strand, within Pf6N2E2_1307at 1541.801 kb on + strand, within Pf6N2E2_1307at 1541.801 kb on + strand, within Pf6N2E2_1307at 1541.801 kb on + strand, within Pf6N2E2_1307at 1541.802 kb on - strand, within Pf6N2E2_1307at 1541.802 kb on - strand, within Pf6N2E2_1307at 1541.802 kb on - strand, within Pf6N2E2_1307at 1541.802 kb on - strand, within Pf6N2E2_1307at 1541.802 kb on - strand, within Pf6N2E2_1307at 1541.802 kb on - strand, within Pf6N2E2_1307at 1541.912 kb on + strand, within Pf6N2E2_1307at 1541.976 kb on + strand, within Pf6N2E2_1307at 1541.976 kb on + strand, within Pf6N2E2_1307at 1541.976 kb on + strand, within Pf6N2E2_1307at 1541.976 kb on + strand, within Pf6N2E2_1307at 1541.976 kb on + strand, within Pf6N2E2_1307at 1542.058 kb on - strand, within Pf6N2E2_1307at 1542.112 kb on + strandat 1542.377 kb on - strand, within Pf6N2E2_1308at 1542.421 kb on + strand, within Pf6N2E2_1308at 1542.422 kb on - strand, within Pf6N2E2_1308at 1542.422 kb on - strand, within Pf6N2E2_1308at 1542.431 kb on - strand, within Pf6N2E2_1308at 1542.545 kb on - strand, within Pf6N2E2_1308at 1542.559 kb on + strand, within Pf6N2E2_1308at 1542.560 kb on - strand, within Pf6N2E2_1308at 1542.739 kb on + strand, within Pf6N2E2_1308at 1542.739 kb on + strand, within Pf6N2E2_1308at 1542.979 kb on - strandat 1543.308 kb on + strand, within Pf6N2E2_1309at 1543.318 kb on - strand, within Pf6N2E2_1309at 1543.360 kb on + strand, within Pf6N2E2_1309at 1543.361 kb on - strand, within Pf6N2E2_1309at 1543.501 kb on + strand, within Pf6N2E2_1309at 1543.565 kb on - strand, within Pf6N2E2_1309at 1543.908 kb on + strand, within Pf6N2E2_1309

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Per-strain Table

Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M
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1,541,206 - -0.2
1,541,206 - -0.1
1,541,390 + -2.1
1,541,407 - +1.6
1,541,751 - Pf6N2E2_1307 0.41 +0.2
1,541,801 + Pf6N2E2_1307 0.49 -1.2
1,541,801 + Pf6N2E2_1307 0.49 -1.8
1,541,801 + Pf6N2E2_1307 0.49 -1.4
1,541,802 - Pf6N2E2_1307 0.49 -2.3
1,541,802 - Pf6N2E2_1307 0.49 -0.7
1,541,802 - Pf6N2E2_1307 0.49 +0.0
1,541,802 - Pf6N2E2_1307 0.49 +1.3
1,541,802 - Pf6N2E2_1307 0.49 -0.9
1,541,802 - Pf6N2E2_1307 0.49 -2.8
1,541,912 + Pf6N2E2_1307 0.65 -2.2
1,541,976 + Pf6N2E2_1307 0.74 -1.9
1,541,976 + Pf6N2E2_1307 0.74 -1.6
1,541,976 + Pf6N2E2_1307 0.74 +0.4
1,541,976 + Pf6N2E2_1307 0.74 +1.7
1,541,976 + Pf6N2E2_1307 0.74 -0.8
1,542,058 - Pf6N2E2_1307 0.87 +2.1
1,542,112 + +0.2
1,542,377 - Pf6N2E2_1308 0.30 +2.1
1,542,421 + Pf6N2E2_1308 0.36 -1.4
1,542,422 - Pf6N2E2_1308 0.36 +1.6
1,542,422 - Pf6N2E2_1308 0.36 -2.8
1,542,431 - Pf6N2E2_1308 0.37 -1.4
1,542,545 - Pf6N2E2_1308 0.52 -0.1
1,542,559 + Pf6N2E2_1308 0.54 -1.8
1,542,560 - Pf6N2E2_1308 0.54 -1.9
1,542,739 + Pf6N2E2_1308 0.77 +1.9
1,542,739 + Pf6N2E2_1308 0.77 -1.4
1,542,979 - -1.4
1,543,308 + Pf6N2E2_1309 0.26 +0.8
1,543,318 - Pf6N2E2_1309 0.27 +1.6
1,543,360 + Pf6N2E2_1309 0.30 -2.4
1,543,361 - Pf6N2E2_1309 0.30 +0.5
1,543,501 + Pf6N2E2_1309 0.40 -2.4
1,543,565 - Pf6N2E2_1309 0.44 +0.3
1,543,908 + Pf6N2E2_1309 0.68 -2.4

Or see this region's nucleotide sequence