Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1283 and Pf6N2E2_1284 are separated by 35 nucleotides Pf6N2E2_1284 and Pf6N2E2_1285 are separated by 10 nucleotides Pf6N2E2_1285 and Pf6N2E2_1286 are separated by 17 nucleotides
Pf6N2E2_1283 - Ferric siderophore transport system, periplasmic binding protein TonB, at 1,513,207 to 1,513,878
_1283
Pf6N2E2_1284 - Biopolymer transport protein ExbD/TolR, at 1,513,914 to 1,514,339
_1284
Pf6N2E2_1285 - MotA/TolQ/ExbB proton channel family protein, at 1,514,350 to 1,516,149
_1285
Pf6N2E2_1286 - Hemolysin activation/secretion protein associated with VreARI signalling system, at 1,516,167 to 1,517,771
_1286
Position (kb) 1514
1515
1516
1517 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1513.472 kb on + strand, within Pf6N2E2_1283 at 1513.472 kb on + strand, within Pf6N2E2_1283 at 1513.473 kb on - strand, within Pf6N2E2_1283 at 1513.473 kb on - strand, within Pf6N2E2_1283 at 1514.002 kb on + strand, within Pf6N2E2_1284 at 1514.328 kb on + strand at 1514.508 kb on - strand at 1514.508 kb on - strand at 1514.634 kb on + strand, within Pf6N2E2_1285 at 1514.635 kb on - strand, within Pf6N2E2_1285 at 1514.635 kb on - strand, within Pf6N2E2_1285 at 1514.635 kb on - strand, within Pf6N2E2_1285 at 1514.635 kb on - strand, within Pf6N2E2_1285 at 1514.843 kb on + strand, within Pf6N2E2_1285 at 1514.843 kb on + strand, within Pf6N2E2_1285 at 1514.844 kb on - strand, within Pf6N2E2_1285 at 1514.844 kb on - strand, within Pf6N2E2_1285 at 1514.845 kb on + strand, within Pf6N2E2_1285 at 1514.846 kb on - strand, within Pf6N2E2_1285 at 1515.122 kb on + strand, within Pf6N2E2_1285 at 1515.329 kb on + strand, within Pf6N2E2_1285 at 1515.531 kb on + strand, within Pf6N2E2_1285 at 1515.719 kb on + strand, within Pf6N2E2_1285 at 1515.720 kb on - strand, within Pf6N2E2_1285 at 1515.720 kb on - strand, within Pf6N2E2_1285 at 1515.791 kb on + strand, within Pf6N2E2_1285 at 1515.791 kb on + strand, within Pf6N2E2_1285 at 1515.791 kb on + strand, within Pf6N2E2_1285 at 1515.791 kb on + strand, within Pf6N2E2_1285 at 1515.791 kb on + strand, within Pf6N2E2_1285 at 1515.792 kb on - strand, within Pf6N2E2_1285 at 1516.123 kb on - strand at 1516.138 kb on + strand at 1516.138 kb on + strand at 1516.379 kb on - strand, within Pf6N2E2_1286 at 1516.399 kb on + strand, within Pf6N2E2_1286 at 1516.399 kb on + strand, within Pf6N2E2_1286 at 1516.606 kb on + strand, within Pf6N2E2_1286 at 1516.768 kb on + strand, within Pf6N2E2_1286 at 1516.789 kb on + strand, within Pf6N2E2_1286 at 1516.903 kb on + strand, within Pf6N2E2_1286 at 1516.921 kb on + strand, within Pf6N2E2_1286 at 1516.922 kb on - strand, within Pf6N2E2_1286 at 1516.922 kb on - strand, within Pf6N2E2_1286 at 1517.122 kb on + strand, within Pf6N2E2_1286 at 1517.122 kb on + strand, within Pf6N2E2_1286
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 1,513,472 + Pf6N2E2_1283 0.39 -1.6 1,513,472 + Pf6N2E2_1283 0.39 +1.8 1,513,473 - Pf6N2E2_1283 0.40 +2.7 1,513,473 - Pf6N2E2_1283 0.40 -0.0 1,514,002 + Pf6N2E2_1284 0.21 -1.9 1,514,328 + -0.8 1,514,508 - -2.1 1,514,508 - -2.9 1,514,634 + Pf6N2E2_1285 0.16 -1.9 1,514,635 - Pf6N2E2_1285 0.16 +0.5 1,514,635 - Pf6N2E2_1285 0.16 -0.9 1,514,635 - Pf6N2E2_1285 0.16 -0.0 1,514,635 - Pf6N2E2_1285 0.16 -0.9 1,514,843 + Pf6N2E2_1285 0.27 -0.9 1,514,843 + Pf6N2E2_1285 0.27 -1.6 1,514,844 - Pf6N2E2_1285 0.27 -0.2 1,514,844 - Pf6N2E2_1285 0.27 -1.2 1,514,845 + Pf6N2E2_1285 0.28 +1.7 1,514,846 - Pf6N2E2_1285 0.28 -2.8 1,515,122 + Pf6N2E2_1285 0.43 -1.6 1,515,329 + Pf6N2E2_1285 0.54 +1.5 1,515,531 + Pf6N2E2_1285 0.66 +2.5 1,515,719 + Pf6N2E2_1285 0.76 -0.4 1,515,720 - Pf6N2E2_1285 0.76 +0.5 1,515,720 - Pf6N2E2_1285 0.76 +0.4 1,515,791 + Pf6N2E2_1285 0.80 -0.8 1,515,791 + Pf6N2E2_1285 0.80 -0.8 1,515,791 + Pf6N2E2_1285 0.80 +0.4 1,515,791 + Pf6N2E2_1285 0.80 -1.4 1,515,791 + Pf6N2E2_1285 0.80 -2.3 1,515,792 - Pf6N2E2_1285 0.80 -2.1 1,516,123 - -0.9 1,516,138 + +0.8 1,516,138 + -1.9 1,516,379 - Pf6N2E2_1286 0.13 -2.1 1,516,399 + Pf6N2E2_1286 0.14 +0.7 1,516,399 + Pf6N2E2_1286 0.14 +1.8 1,516,606 + Pf6N2E2_1286 0.27 +2.4 1,516,768 + Pf6N2E2_1286 0.37 +0.8 1,516,789 + Pf6N2E2_1286 0.39 +2.2 1,516,903 + Pf6N2E2_1286 0.46 +0.5 1,516,921 + Pf6N2E2_1286 0.47 -1.9 1,516,922 - Pf6N2E2_1286 0.47 -0.0 1,516,922 - Pf6N2E2_1286 0.47 +0.7 1,517,122 + Pf6N2E2_1286 0.60 -1.6 1,517,122 + Pf6N2E2_1286 0.60 -2.9
Or see this region's nucleotide sequence