Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1076 and Pf6N2E2_1077 overlap by 4 nucleotides Pf6N2E2_1077 and Pf6N2E2_1078 are separated by 49 nucleotides
Pf6N2E2_1076 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 1,257,423 to 1,259,501
_1076
Pf6N2E2_1077 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 1,259,498 to 1,261,258
_1077
Pf6N2E2_1078 - Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21), at 1,261,308 to 1,262,879
_1078
Position (kb)
1259
1260
1261
1262 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1258.874 kb on - strand, within Pf6N2E2_1076 at 1258.874 kb on - strand, within Pf6N2E2_1076 at 1259.023 kb on + strand, within Pf6N2E2_1076 at 1259.023 kb on + strand, within Pf6N2E2_1076 at 1259.024 kb on - strand, within Pf6N2E2_1076 at 1259.024 kb on - strand, within Pf6N2E2_1076 at 1259.024 kb on - strand, within Pf6N2E2_1076 at 1259.087 kb on - strand, within Pf6N2E2_1076 at 1259.251 kb on + strand, within Pf6N2E2_1076 at 1259.252 kb on - strand, within Pf6N2E2_1076 at 1259.252 kb on - strand, within Pf6N2E2_1076 at 1260.005 kb on - strand, within Pf6N2E2_1077 at 1260.071 kb on + strand, within Pf6N2E2_1077 at 1260.307 kb on - strand, within Pf6N2E2_1077 at 1260.315 kb on + strand, within Pf6N2E2_1077 at 1260.315 kb on + strand, within Pf6N2E2_1077 at 1260.318 kb on + strand, within Pf6N2E2_1077 at 1260.319 kb on - strand, within Pf6N2E2_1077 at 1260.387 kb on + strand, within Pf6N2E2_1077 at 1260.387 kb on + strand, within Pf6N2E2_1077 at 1260.388 kb on - strand, within Pf6N2E2_1077 at 1260.519 kb on - strand, within Pf6N2E2_1077 at 1260.549 kb on + strand, within Pf6N2E2_1077 at 1260.550 kb on - strand, within Pf6N2E2_1077 at 1260.678 kb on + strand, within Pf6N2E2_1077 at 1260.678 kb on + strand, within Pf6N2E2_1077 at 1260.678 kb on + strand, within Pf6N2E2_1077 at 1260.678 kb on + strand, within Pf6N2E2_1077 at 1260.678 kb on + strand, within Pf6N2E2_1077 at 1260.678 kb on + strand, within Pf6N2E2_1077 at 1260.705 kb on + strand, within Pf6N2E2_1077 at 1260.705 kb on + strand, within Pf6N2E2_1077 at 1260.705 kb on + strand, within Pf6N2E2_1077 at 1260.705 kb on + strand, within Pf6N2E2_1077 at 1260.706 kb on - strand, within Pf6N2E2_1077 at 1261.005 kb on + strand, within Pf6N2E2_1077 at 1261.101 kb on + strand at 1261.102 kb on - strand at 1261.304 kb on + strand at 1261.305 kb on - strand at 1261.337 kb on + strand at 1261.337 kb on + strand at 1261.355 kb on - strand at 1261.355 kb on - strand at 1261.366 kb on + strand at 1261.366 kb on + strand at 1261.481 kb on - strand, within Pf6N2E2_1078 at 1261.481 kb on - strand, within Pf6N2E2_1078 at 1261.591 kb on + strand, within Pf6N2E2_1078 at 1261.592 kb on - strand, within Pf6N2E2_1078 at 1261.592 kb on - strand, within Pf6N2E2_1078 at 1262.095 kb on + strand, within Pf6N2E2_1078 at 1262.095 kb on + strand, within Pf6N2E2_1078 at 1262.095 kb on + strand, within Pf6N2E2_1078 at 1262.095 kb on + strand, within Pf6N2E2_1078 at 1262.095 kb on + strand, within Pf6N2E2_1078 at 1262.096 kb on - strand, within Pf6N2E2_1078 at 1262.096 kb on - strand, within Pf6N2E2_1078 at 1262.096 kb on - strand, within Pf6N2E2_1078 at 1262.117 kb on + strand, within Pf6N2E2_1078 at 1262.117 kb on + strand, within Pf6N2E2_1078 at 1262.117 kb on + strand, within Pf6N2E2_1078 at 1262.128 kb on + strand, within Pf6N2E2_1078 at 1262.128 kb on + strand, within Pf6N2E2_1078 at 1262.129 kb on - strand, within Pf6N2E2_1078 at 1262.129 kb on - strand, within Pf6N2E2_1078 at 1262.165 kb on - strand, within Pf6N2E2_1078
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 1,258,874 - Pf6N2E2_1076 0.70 -2.5 1,258,874 - Pf6N2E2_1076 0.70 +2.8 1,259,023 + Pf6N2E2_1076 0.77 -0.1 1,259,023 + Pf6N2E2_1076 0.77 -0.4 1,259,024 - Pf6N2E2_1076 0.77 -2.3 1,259,024 - Pf6N2E2_1076 0.77 -0.6 1,259,024 - Pf6N2E2_1076 0.77 +1.2 1,259,087 - Pf6N2E2_1076 0.80 +0.3 1,259,251 + Pf6N2E2_1076 0.88 -0.1 1,259,252 - Pf6N2E2_1076 0.88 +0.9 1,259,252 - Pf6N2E2_1076 0.88 +1.4 1,260,005 - Pf6N2E2_1077 0.29 -1.7 1,260,071 + Pf6N2E2_1077 0.33 -2.9 1,260,307 - Pf6N2E2_1077 0.46 -2.8 1,260,315 + Pf6N2E2_1077 0.46 -0.4 1,260,315 + Pf6N2E2_1077 0.46 +2.6 1,260,318 + Pf6N2E2_1077 0.47 -0.4 1,260,319 - Pf6N2E2_1077 0.47 +2.1 1,260,387 + Pf6N2E2_1077 0.50 -1.5 1,260,387 + Pf6N2E2_1077 0.50 +0.5 1,260,388 - Pf6N2E2_1077 0.51 -2.9 1,260,519 - Pf6N2E2_1077 0.58 -2.5 1,260,549 + Pf6N2E2_1077 0.60 -2.5 1,260,550 - Pf6N2E2_1077 0.60 -0.9 1,260,678 + Pf6N2E2_1077 0.67 -2.5 1,260,678 + Pf6N2E2_1077 0.67 -0.8 1,260,678 + Pf6N2E2_1077 0.67 -0.2 1,260,678 + Pf6N2E2_1077 0.67 +0.6 1,260,678 + Pf6N2E2_1077 0.67 +0.2 1,260,678 + Pf6N2E2_1077 0.67 -0.9 1,260,705 + Pf6N2E2_1077 0.69 +1.7 1,260,705 + Pf6N2E2_1077 0.69 -1.4 1,260,705 + Pf6N2E2_1077 0.69 +0.4 1,260,705 + Pf6N2E2_1077 0.69 -0.8 1,260,706 - Pf6N2E2_1077 0.69 +3.0 1,261,005 + Pf6N2E2_1077 0.86 -0.4 1,261,101 + +0.3 1,261,102 - -3.1 1,261,304 + -1.1 1,261,305 - -0.2 1,261,337 + +1.7 1,261,337 + -0.5 1,261,355 - +1.5 1,261,355 - +1.3 1,261,366 + -1.0 1,261,366 + -0.1 1,261,481 - Pf6N2E2_1078 0.11 -0.2 1,261,481 - Pf6N2E2_1078 0.11 +1.7 1,261,591 + Pf6N2E2_1078 0.18 -2.2 1,261,592 - Pf6N2E2_1078 0.18 +1.2 1,261,592 - Pf6N2E2_1078 0.18 +1.5 1,262,095 + Pf6N2E2_1078 0.50 -0.1 1,262,095 + Pf6N2E2_1078 0.50 -2.0 1,262,095 + Pf6N2E2_1078 0.50 -2.5 1,262,095 + Pf6N2E2_1078 0.50 +0.7 1,262,095 + Pf6N2E2_1078 0.50 -1.1 1,262,096 - Pf6N2E2_1078 0.50 +0.8 1,262,096 - Pf6N2E2_1078 0.50 +1.1 1,262,096 - Pf6N2E2_1078 0.50 -2.1 1,262,117 + Pf6N2E2_1078 0.51 +1.8 1,262,117 + Pf6N2E2_1078 0.51 -3.7 1,262,117 + Pf6N2E2_1078 0.51 +0.3 1,262,128 + Pf6N2E2_1078 0.52 +2.1 1,262,128 + Pf6N2E2_1078 0.52 -0.1 1,262,129 - Pf6N2E2_1078 0.52 +0.9 1,262,129 - Pf6N2E2_1078 0.52 +3.0 1,262,165 - Pf6N2E2_1078 0.55 -0.5
Or see this region's nucleotide sequence