Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08375

Experiment: Uridine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08370 and BWI76_RS08375 are separated by 9 nucleotidesBWI76_RS08375 and BWI76_RS08380 are separated by 15 nucleotidesBWI76_RS08380 and BWI76_RS08385 are separated by 45 nucleotides BWI76_RS08370: BWI76_RS08370 - membrane protein, at 1,771,176 to 1,772,171 _RS08370 BWI76_RS08375: BWI76_RS08375 - pyroglutamyl-peptidase I, at 1,772,181 to 1,772,825 _RS08375 BWI76_RS08380: BWI76_RS08380 - endonuclease VIII, at 1,772,841 to 1,773,632 _RS08380 BWI76_RS08385: BWI76_RS08385 - citrate (Si)-synthase, at 1,773,678 to 1,774,961 _RS08385 Position (kb) 1772 1773Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1771.234 kb on - strandat 1771.286 kb on + strand, within BWI76_RS08370at 1771.330 kb on + strand, within BWI76_RS08370at 1772.016 kb on + strand, within BWI76_RS08370at 1772.108 kb on + strandat 1772.116 kb on + strandat 1772.627 kb on - strand, within BWI76_RS08375at 1772.633 kb on + strand, within BWI76_RS08375at 1772.904 kb on + strandat 1772.912 kb on - strandat 1773.069 kb on + strand, within BWI76_RS08380at 1773.220 kb on + strand, within BWI76_RS08380at 1773.794 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Uridine (N)
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1,771,234 - -0.8
1,771,286 + BWI76_RS08370 0.11 +0.6
1,771,330 + BWI76_RS08370 0.15 -1.8
1,772,016 + BWI76_RS08370 0.84 +0.9
1,772,108 + +0.9
1,772,116 + +0.6
1,772,627 - BWI76_RS08375 0.69 -1.7
1,772,633 + BWI76_RS08375 0.70 -4.4
1,772,904 + -0.2
1,772,912 - -0.3
1,773,069 + BWI76_RS08380 0.29 -0.2
1,773,220 + BWI76_RS08380 0.48 +0.0
1,773,794 + -0.9

Or see this region's nucleotide sequence