Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02215

Experiment: Uridine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02205 and BWI76_RS02210 overlap by 4 nucleotidesBWI76_RS02210 and BWI76_RS02215 overlap by 8 nucleotidesBWI76_RS02215 and BWI76_RS02220 overlap by 1 nucleotidesBWI76_RS02220 and BWI76_RS02225 overlap by 4 nucleotides BWI76_RS02205: BWI76_RS02205 - phosphonate C-P lyase system protein PhnK, at 456,761 to 457,519 _RS02205 BWI76_RS02210: BWI76_RS02210 - carbon-phosphorus lyase complex subunit PhnJ, at 457,516 to 458,364 _RS02210 BWI76_RS02215: BWI76_RS02215 - carbon-phosphorus lyase complex subunit PhnI, at 458,357 to 459,421 _RS02215 BWI76_RS02220: BWI76_RS02220 - carbon-phosphorus lyase subunit PhnH, at 459,421 to 460,005 _RS02220 BWI76_RS02225: BWI76_RS02225 - phosphonate C-P lyase system protein PhnG, at 460,002 to 460,454 _RS02225 Position (kb) 458 459 460Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 457.436 kb on + strand, within BWI76_RS02205at 457.473 kb on + strandat 457.637 kb on + strand, within BWI76_RS02210at 457.654 kb on + strand, within BWI76_RS02210at 457.701 kb on + strandat 457.718 kb on + strand, within BWI76_RS02210at 457.752 kb on + strand, within BWI76_RS02210at 457.760 kb on - strand, within BWI76_RS02210at 457.826 kb on + strand, within BWI76_RS02210at 457.854 kb on - strand, within BWI76_RS02210at 457.854 kb on - strand, within BWI76_RS02210at 457.854 kb on - strand, within BWI76_RS02210at 457.925 kb on - strand, within BWI76_RS02210at 457.974 kb on + strand, within BWI76_RS02210at 458.223 kb on - strand, within BWI76_RS02210at 458.385 kb on + strandat 458.385 kb on + strandat 458.393 kb on - strandat 458.393 kb on - strandat 458.393 kb on - strandat 458.523 kb on - strand, within BWI76_RS02215at 458.601 kb on - strand, within BWI76_RS02215at 458.670 kb on + strand, within BWI76_RS02215at 458.775 kb on - strand, within BWI76_RS02215at 458.788 kb on + strand, within BWI76_RS02215at 458.793 kb on + strand, within BWI76_RS02215at 458.796 kb on - strand, within BWI76_RS02215at 458.796 kb on - strand, within BWI76_RS02215at 458.801 kb on - strand, within BWI76_RS02215at 458.801 kb on - strand, within BWI76_RS02215at 458.864 kb on + strand, within BWI76_RS02215at 458.872 kb on - strand, within BWI76_RS02215at 459.224 kb on - strand, within BWI76_RS02215at 459.427 kb on - strandat 459.794 kb on + strand, within BWI76_RS02220at 459.804 kb on + strand, within BWI76_RS02220at 459.906 kb on + strand, within BWI76_RS02220at 460.008 kb on - strandat 460.181 kb on - strand, within BWI76_RS02225at 460.182 kb on - strand, within BWI76_RS02225at 460.319 kb on + strand, within BWI76_RS02225at 460.342 kb on + strand, within BWI76_RS02225

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Per-strain Table

Position Strand Gene LocusTag Fraction Uridine (N)
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457,436 + BWI76_RS02205 0.89 +0.6
457,473 + +0.4
457,637 + BWI76_RS02210 0.14 +1.1
457,654 + BWI76_RS02210 0.16 -0.5
457,701 + +0.7
457,718 + BWI76_RS02210 0.24 -0.4
457,752 + BWI76_RS02210 0.28 -1.3
457,760 - BWI76_RS02210 0.29 +0.7
457,826 + BWI76_RS02210 0.37 -4.3
457,854 - BWI76_RS02210 0.40 +2.1
457,854 - BWI76_RS02210 0.40 -0.8
457,854 - BWI76_RS02210 0.40 -1.2
457,925 - BWI76_RS02210 0.48 -2.0
457,974 + BWI76_RS02210 0.54 +0.6
458,223 - BWI76_RS02210 0.83 +0.7
458,385 + +0.4
458,385 + +1.6
458,393 - -2.4
458,393 - -2.2
458,393 - -3.6
458,523 - BWI76_RS02215 0.16 +0.5
458,601 - BWI76_RS02215 0.23 -1.4
458,670 + BWI76_RS02215 0.29 -1.9
458,775 - BWI76_RS02215 0.39 -1.5
458,788 + BWI76_RS02215 0.40 -2.4
458,793 + BWI76_RS02215 0.41 -0.4
458,796 - BWI76_RS02215 0.41 +0.3
458,796 - BWI76_RS02215 0.41 -2.0
458,801 - BWI76_RS02215 0.42 -3.6
458,801 - BWI76_RS02215 0.42 -0.6
458,864 + BWI76_RS02215 0.48 -0.7
458,872 - BWI76_RS02215 0.48 +0.6
459,224 - BWI76_RS02215 0.81 +0.6
459,427 - -2.0
459,794 + BWI76_RS02220 0.64 +0.0
459,804 + BWI76_RS02220 0.65 -3.8
459,906 + BWI76_RS02220 0.83 -2.3
460,008 - +0.4
460,181 - BWI76_RS02225 0.40 -0.1
460,182 - BWI76_RS02225 0.40 -2.1
460,319 + BWI76_RS02225 0.70 -1.9
460,342 + BWI76_RS02225 0.75 -2.8

Or see this region's nucleotide sequence