Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00885

Experiment: Uridine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00875 and BWI76_RS00880 are separated by 15 nucleotidesBWI76_RS00880 and BWI76_RS00885 are separated by 46 nucleotidesBWI76_RS00885 and BWI76_RS00890 are separated by 364 nucleotides BWI76_RS00875: BWI76_RS00875 - DNA-binding transcriptional regulator FabR, at 180,927 to 181,562 _RS00875 BWI76_RS00880: BWI76_RS00880 - membrane protein, at 181,578 to 181,937 _RS00880 BWI76_RS00885: BWI76_RS00885 - tRNA (uridine(54)-C5)-methyltransferase TrmA, at 181,984 to 183,084 _RS00885 BWI76_RS00890: BWI76_RS00890 - vitamin B12/cobalamin outer membrane transporter, at 183,449 to 185,317 _RS00890 Position (kb) 181 182 183 184Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 181.021 kb on + strand, within BWI76_RS00875at 181.021 kb on + strand, within BWI76_RS00875at 181.021 kb on + strand, within BWI76_RS00875at 181.109 kb on + strand, within BWI76_RS00875at 181.129 kb on + strand, within BWI76_RS00875at 181.294 kb on + strand, within BWI76_RS00875at 181.383 kb on + strand, within BWI76_RS00875at 181.388 kb on + strand, within BWI76_RS00875at 181.396 kb on - strand, within BWI76_RS00875at 181.444 kb on + strand, within BWI76_RS00875at 181.586 kb on - strandat 181.590 kb on + strandat 181.590 kb on + strandat 181.590 kb on + strandat 181.590 kb on + strandat 181.590 kb on + strandat 181.595 kb on + strandat 181.595 kb on + strandat 181.598 kb on - strandat 181.603 kb on - strandat 181.659 kb on + strand, within BWI76_RS00880at 181.661 kb on + strand, within BWI76_RS00880at 181.661 kb on + strand, within BWI76_RS00880at 181.886 kb on + strand, within BWI76_RS00880at 182.436 kb on + strand, within BWI76_RS00885at 182.439 kb on - strand, within BWI76_RS00885at 182.575 kb on - strand, within BWI76_RS00885at 182.637 kb on + strand, within BWI76_RS00885at 182.713 kb on + strand, within BWI76_RS00885at 182.772 kb on + strand, within BWI76_RS00885at 182.812 kb on - strand, within BWI76_RS00885at 183.052 kb on + strandat 183.062 kb on + strandat 183.078 kb on - strandat 183.079 kb on + strandat 183.121 kb on - strandat 183.129 kb on + strandat 183.140 kb on + strandat 183.140 kb on + strandat 183.140 kb on + strandat 183.145 kb on + strandat 183.231 kb on + strandat 183.236 kb on + strandat 183.290 kb on - strandat 183.328 kb on + strandat 183.354 kb on - strandat 183.360 kb on - strandat 183.413 kb on - strandat 183.419 kb on - strandat 183.428 kb on + strandat 183.527 kb on - strandat 183.535 kb on + strandat 183.536 kb on - strandat 183.567 kb on + strandat 183.613 kb on - strandat 183.753 kb on - strand, within BWI76_RS00890at 183.758 kb on + strand, within BWI76_RS00890at 183.786 kb on + strand, within BWI76_RS00890at 183.830 kb on + strand, within BWI76_RS00890at 183.872 kb on + strand, within BWI76_RS00890at 183.880 kb on - strand, within BWI76_RS00890at 183.894 kb on - strand, within BWI76_RS00890at 183.942 kb on + strand, within BWI76_RS00890at 183.942 kb on + strand, within BWI76_RS00890at 183.942 kb on + strand, within BWI76_RS00890at 183.945 kb on - strand, within BWI76_RS00890at 183.951 kb on - strand, within BWI76_RS00890at 184.005 kb on - strand, within BWI76_RS00890at 184.017 kb on + strand, within BWI76_RS00890at 184.017 kb on + strand, within BWI76_RS00890at 184.066 kb on + strand, within BWI76_RS00890

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Per-strain Table

Position Strand Gene LocusTag Fraction Uridine (N)
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181,021 + BWI76_RS00875 0.15 -0.4
181,021 + BWI76_RS00875 0.15 +0.0
181,021 + BWI76_RS00875 0.15 -0.9
181,109 + BWI76_RS00875 0.29 -1.6
181,129 + BWI76_RS00875 0.32 -0.5
181,294 + BWI76_RS00875 0.58 -1.1
181,383 + BWI76_RS00875 0.72 +1.0
181,388 + BWI76_RS00875 0.72 +0.8
181,396 - BWI76_RS00875 0.74 -1.0
181,444 + BWI76_RS00875 0.81 -2.4
181,586 - -1.0
181,590 + +0.6
181,590 + +2.4
181,590 + -0.9
181,590 + -0.9
181,590 + -1.9
181,595 + +0.8
181,595 + +0.3
181,598 - +1.5
181,603 - +0.9
181,659 + BWI76_RS00880 0.23 +0.7
181,661 + BWI76_RS00880 0.23 -0.1
181,661 + BWI76_RS00880 0.23 -0.9
181,886 + BWI76_RS00880 0.86 +1.3
182,436 + BWI76_RS00885 0.41 +0.1
182,439 - BWI76_RS00885 0.41 +1.3
182,575 - BWI76_RS00885 0.54 -2.1
182,637 + BWI76_RS00885 0.59 -0.9
182,713 + BWI76_RS00885 0.66 +1.3
182,772 + BWI76_RS00885 0.72 +0.9
182,812 - BWI76_RS00885 0.75 +1.2
183,052 + -1.1
183,062 + -2.6
183,078 - -2.0
183,079 + -0.5
183,121 - -0.9
183,129 + +3.1
183,140 + -3.3
183,140 + -3.5
183,140 + -0.9
183,145 + +0.1
183,231 + -0.3
183,236 + +0.1
183,290 - -1.9
183,328 + +0.3
183,354 - +3.3
183,360 - +1.2
183,413 - +1.8
183,419 - +1.6
183,428 + -1.4
183,527 - +1.5
183,535 + +0.7
183,536 - +0.7
183,567 + +2.0
183,613 - +2.8
183,753 - BWI76_RS00890 0.16 +0.3
183,758 + BWI76_RS00890 0.17 +0.1
183,786 + BWI76_RS00890 0.18 -0.7
183,830 + BWI76_RS00890 0.20 +0.9
183,872 + BWI76_RS00890 0.23 +1.2
183,880 - BWI76_RS00890 0.23 +1.8
183,894 - BWI76_RS00890 0.24 +0.4
183,942 + BWI76_RS00890 0.26 +1.0
183,942 + BWI76_RS00890 0.26 +0.8
183,942 + BWI76_RS00890 0.26 -0.3
183,945 - BWI76_RS00890 0.27 +1.2
183,951 - BWI76_RS00890 0.27 +0.2
184,005 - BWI76_RS00890 0.30 +0.5
184,017 + BWI76_RS00890 0.30 +0.9
184,017 + BWI76_RS00890 0.30 +1.6
184,066 + BWI76_RS00890 0.33 +2.7

Or see this region's nucleotide sequence