Experiment: reconstituted MRS with Porphyran 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14065 and BBR_RS14070 are separated by 615 nucleotides BBR_RS14070 and BBR_RS14075 are separated by 387 nucleotides
BBR_RS14065: BBR_RS14065 - TnpV protein, at 930,308 to 930,681
_RS14065
BBR_RS14070: BBR_RS14070 - threonine synthase, at 931,297 to 932,787
_RS14070
BBR_RS14075: BBR_RS14075 - glutamate-5-semialdehyde dehydrogenase, at 933,175 to 934,470
_RS14075
Position (kb)
931
932
933 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 930.328 kb on + strand at 930.328 kb on + strand at 930.337 kb on + strand at 930.339 kb on + strand at 930.345 kb on - strand at 930.345 kb on - strand at 930.365 kb on + strand, within BBR_RS14065 at 930.393 kb on + strand, within BBR_RS14065 at 930.606 kb on + strand, within BBR_RS14065 at 930.606 kb on + strand, within BBR_RS14065 at 930.633 kb on + strand, within BBR_RS14065 at 930.643 kb on + strand, within BBR_RS14065 at 930.662 kb on - strand at 930.666 kb on + strand at 930.666 kb on + strand at 930.700 kb on + strand at 930.700 kb on + strand at 930.700 kb on + strand at 930.757 kb on + strand at 930.850 kb on + strand at 930.855 kb on + strand at 930.912 kb on - strand at 930.913 kb on - strand at 930.913 kb on - strand at 930.913 kb on - strand at 930.913 kb on - strand at 930.926 kb on - strand at 930.949 kb on + strand at 930.957 kb on - strand at 930.957 kb on - strand at 931.034 kb on - strand at 931.051 kb on - strand at 931.061 kb on + strand at 931.069 kb on - strand at 931.075 kb on + strand at 931.083 kb on - strand at 931.083 kb on - strand at 931.093 kb on - strand at 931.142 kb on - strand at 931.175 kb on - strand at 931.255 kb on - strand at 931.279 kb on - strand at 931.292 kb on + strand at 931.292 kb on + strand at 931.294 kb on + strand at 931.294 kb on + strand at 931.302 kb on - strand at 931.302 kb on - strand at 931.327 kb on + strand at 931.327 kb on + strand at 931.337 kb on - strand at 931.337 kb on - strand at 931.527 kb on - strand, within BBR_RS14070 at 931.565 kb on - strand, within BBR_RS14070 at 931.652 kb on - strand, within BBR_RS14070 at 931.652 kb on - strand, within BBR_RS14070 at 931.653 kb on - strand, within BBR_RS14070 at 931.698 kb on - strand, within BBR_RS14070 at 931.725 kb on + strand, within BBR_RS14070 at 931.733 kb on - strand, within BBR_RS14070 at 931.772 kb on + strand, within BBR_RS14070 at 931.785 kb on - strand, within BBR_RS14070 at 931.800 kb on - strand, within BBR_RS14070 at 931.800 kb on - strand, within BBR_RS14070 at 931.835 kb on + strand, within BBR_RS14070 at 931.858 kb on + strand, within BBR_RS14070 at 931.858 kb on + strand, within BBR_RS14070 at 932.067 kb on - strand, within BBR_RS14070 at 932.121 kb on + strand, within BBR_RS14070 at 932.155 kb on - strand, within BBR_RS14070 at 932.159 kb on - strand, within BBR_RS14070 at 932.228 kb on - strand, within BBR_RS14070 at 932.264 kb on + strand, within BBR_RS14070 at 932.270 kb on - strand, within BBR_RS14070 at 932.285 kb on + strand, within BBR_RS14070 at 932.295 kb on + strand, within BBR_RS14070 at 932.405 kb on + strand, within BBR_RS14070 at 932.448 kb on + strand, within BBR_RS14070 at 932.479 kb on - strand, within BBR_RS14070 at 932.489 kb on - strand, within BBR_RS14070 at 932.529 kb on - strand, within BBR_RS14070 at 932.539 kb on - strand, within BBR_RS14070 at 932.776 kb on - strand at 932.937 kb on + strand at 932.939 kb on + strand at 932.947 kb on - strand at 933.003 kb on + strand at 933.083 kb on - strand at 933.138 kb on - strand at 933.140 kb on + strand at 933.140 kb on + strand at 933.148 kb on - strand at 933.289 kb on + strand at 933.487 kb on - strand, within BBR_RS14075 at 933.494 kb on + strand, within BBR_RS14075 at 933.494 kb on + strand, within BBR_RS14075 at 933.494 kb on + strand, within BBR_RS14075 at 933.671 kb on + strand, within BBR_RS14075 at 933.718 kb on + strand, within BBR_RS14075 at 933.741 kb on + strand, within BBR_RS14075
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Porphyran 0.5 %w/v remove 930,328 + +1.0 930,328 + +0.3 930,337 + +0.1 930,339 + +0.5 930,345 - -1.3 930,345 - -0.5 930,365 + BBR_RS14065 0.15 +0.5 930,393 + BBR_RS14065 0.23 +1.1 930,606 + BBR_RS14065 0.80 +0.1 930,606 + BBR_RS14065 0.80 -0.2 930,633 + BBR_RS14065 0.87 -0.8 930,643 + BBR_RS14065 0.90 +0.7 930,662 - +3.1 930,666 + +0.2 930,666 + +1.0 930,700 + +0.8 930,700 + -0.4 930,700 + -0.7 930,757 + -0.2 930,850 + +0.5 930,855 + +0.4 930,912 - +0.0 930,913 - +0.4 930,913 - -0.0 930,913 - +0.2 930,913 - +0.3 930,926 - +0.1 930,949 + -2.2 930,957 - +0.0 930,957 - -0.2 931,034 - +0.8 931,051 - -0.2 931,061 + -0.1 931,069 - -0.5 931,075 + +1.0 931,083 - -0.4 931,083 - -1.0 931,093 - -0.0 931,142 - -1.2 931,175 - -0.5 931,255 - +0.1 931,279 - +2.5 931,292 + +1.2 931,292 + +0.4 931,294 + +1.3 931,294 + +1.0 931,302 - -0.6 931,302 - -0.4 931,327 + +1.3 931,327 + +1.3 931,337 - -0.4 931,337 - -0.4 931,527 - BBR_RS14070 0.15 +1.0 931,565 - BBR_RS14070 0.18 +0.8 931,652 - BBR_RS14070 0.24 -0.3 931,652 - BBR_RS14070 0.24 +0.7 931,653 - BBR_RS14070 0.24 -0.4 931,698 - BBR_RS14070 0.27 -1.5 931,725 + BBR_RS14070 0.29 -0.4 931,733 - BBR_RS14070 0.29 +0.2 931,772 + BBR_RS14070 0.32 -0.6 931,785 - BBR_RS14070 0.33 -0.7 931,800 - BBR_RS14070 0.34 +0.0 931,800 - BBR_RS14070 0.34 -1.5 931,835 + BBR_RS14070 0.36 +1.0 931,858 + BBR_RS14070 0.38 -0.5 931,858 + BBR_RS14070 0.38 -0.5 932,067 - BBR_RS14070 0.52 +1.0 932,121 + BBR_RS14070 0.55 +0.2 932,155 - BBR_RS14070 0.58 +0.2 932,159 - BBR_RS14070 0.58 +0.4 932,228 - BBR_RS14070 0.62 -0.9 932,264 + BBR_RS14070 0.65 -0.3 932,270 - BBR_RS14070 0.65 +0.1 932,285 + BBR_RS14070 0.66 +0.5 932,295 + BBR_RS14070 0.67 +1.5 932,405 + BBR_RS14070 0.74 -0.9 932,448 + BBR_RS14070 0.77 +0.5 932,479 - BBR_RS14070 0.79 +0.2 932,489 - BBR_RS14070 0.80 +0.0 932,529 - BBR_RS14070 0.83 +0.2 932,539 - BBR_RS14070 0.83 -0.3 932,776 - +0.9 932,937 + -0.7 932,939 + +0.2 932,947 - +0.7 933,003 + +0.3 933,083 - +0.6 933,138 - +0.5 933,140 + +1.0 933,140 + -0.0 933,148 - +0.6 933,289 + -1.3 933,487 - BBR_RS14075 0.24 +0.1 933,494 + BBR_RS14075 0.25 -0.3 933,494 + BBR_RS14075 0.25 -0.7 933,494 + BBR_RS14075 0.25 +1.1 933,671 + BBR_RS14075 0.38 +0.2 933,718 + BBR_RS14075 0.42 -0.3 933,741 + BBR_RS14075 0.44 -0.7
Or see this region's nucleotide sequence