Experiment: Succinic Acid; Light intensity 75 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3007 and Rru_A3008 are separated by 1 nucleotides Rru_A3008 and Rru_A3009 are separated by 334 nucleotides Rru_A3009 and Rru_A3010 are separated by 10 nucleotides Rru_A3010 and Rru_A3011 are separated by 110 nucleotides
Rru_A3007: Rru_A3007 - SOUL heme-binding protein (NCBI), at 3,464,938 to 3,465,531
_A3007
Rru_A3008: Rru_A3008 - Protein of unknown function UPF0153 (NCBI), at 3,465,533 to 3,465,844
_A3008
Rru_A3009: Rru_A3009 - Aminotransferase class-III (NCBI), at 3,466,179 to 3,467,510
_A3009
Rru_A3010: Rru_A3010 - Transcriptional Regulator, DeoR family (NCBI), at 3,467,521 to 3,468,315
_A3010
Rru_A3011: Rru_A3011 - Isochorismatase hydrolase (NCBI), at 3,468,426 to 3,469,133
_A3011
Position (kb)
3466
3467
3468 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3465.457 kb on + strand, within Rru_A3007 at 3465.458 kb on - strand, within Rru_A3007 at 3465.458 kb on - strand, within Rru_A3007 at 3465.458 kb on - strand, within Rru_A3007 at 3465.458 kb on - strand, within Rru_A3007 at 3465.458 kb on - strand, within Rru_A3007 at 3465.458 kb on - strand, within Rru_A3007 at 3465.464 kb on - strand, within Rru_A3007 at 3465.464 kb on - strand, within Rru_A3007 at 3465.464 kb on - strand, within Rru_A3007 at 3465.530 kb on - strand at 3465.648 kb on + strand, within Rru_A3008 at 3465.845 kb on + strand at 3465.845 kb on + strand at 3465.845 kb on + strand at 3465.845 kb on + strand at 3465.846 kb on - strand at 3465.846 kb on - strand at 3466.115 kb on + strand at 3466.299 kb on + strand at 3466.300 kb on - strand at 3466.300 kb on - strand at 3466.304 kb on + strand at 3466.377 kb on + strand, within Rru_A3009 at 3466.378 kb on - strand, within Rru_A3009 at 3466.535 kb on + strand, within Rru_A3009 at 3466.596 kb on + strand, within Rru_A3009 at 3466.596 kb on + strand, within Rru_A3009 at 3466.596 kb on + strand, within Rru_A3009 at 3466.597 kb on - strand, within Rru_A3009 at 3466.646 kb on + strand, within Rru_A3009 at 3466.791 kb on - strand, within Rru_A3009 at 3466.867 kb on - strand, within Rru_A3009 at 3467.109 kb on + strand, within Rru_A3009 at 3467.218 kb on - strand, within Rru_A3009 at 3467.488 kb on + strand at 3467.488 kb on + strand at 3467.488 kb on + strand at 3467.488 kb on + strand at 3467.488 kb on + strand at 3467.488 kb on + strand at 3467.489 kb on - strand at 3467.489 kb on - strand at 3467.489 kb on - strand at 3467.624 kb on + strand, within Rru_A3010 at 3467.705 kb on + strand, within Rru_A3010 at 3467.705 kb on + strand, within Rru_A3010 at 3467.706 kb on - strand, within Rru_A3010 at 3467.706 kb on - strand, within Rru_A3010 at 3467.786 kb on + strand, within Rru_A3010 at 3467.801 kb on + strand, within Rru_A3010 at 3467.801 kb on + strand, within Rru_A3010 at 3467.802 kb on - strand, within Rru_A3010 at 3467.802 kb on - strand, within Rru_A3010 at 3467.802 kb on - strand, within Rru_A3010 at 3467.846 kb on + strand, within Rru_A3010 at 3467.846 kb on + strand, within Rru_A3010 at 3467.847 kb on - strand, within Rru_A3010 at 3468.119 kb on + strand, within Rru_A3010 at 3468.119 kb on + strand, within Rru_A3010 at 3468.119 kb on + strand, within Rru_A3010 at 3468.120 kb on - strand, within Rru_A3010 at 3468.120 kb on - strand, within Rru_A3010 at 3468.474 kb on + strand at 3468.475 kb on - strand at 3468.475 kb on - strand at 3468.475 kb on - strand at 3468.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 75 uM remove 3,465,457 + Rru_A3007 0.87 +0.6 3,465,458 - Rru_A3007 0.88 +1.0 3,465,458 - Rru_A3007 0.88 -1.0 3,465,458 - Rru_A3007 0.88 +0.0 3,465,458 - Rru_A3007 0.88 +0.2 3,465,458 - Rru_A3007 0.88 +0.5 3,465,458 - Rru_A3007 0.88 +0.7 3,465,464 - Rru_A3007 0.89 +0.0 3,465,464 - Rru_A3007 0.89 +0.2 3,465,464 - Rru_A3007 0.89 +1.5 3,465,530 - +0.9 3,465,648 + Rru_A3008 0.37 -0.1 3,465,845 + +0.8 3,465,845 + +0.0 3,465,845 + +2.2 3,465,845 + +0.0 3,465,846 - +1.6 3,465,846 - -1.0 3,466,115 + +0.0 3,466,299 + +0.0 3,466,300 - -2.4 3,466,300 - +0.0 3,466,304 + +0.0 3,466,377 + Rru_A3009 0.15 +0.0 3,466,378 - Rru_A3009 0.15 +0.0 3,466,535 + Rru_A3009 0.27 +0.0 3,466,596 + Rru_A3009 0.31 -1.0 3,466,596 + Rru_A3009 0.31 +0.0 3,466,596 + Rru_A3009 0.31 -1.0 3,466,597 - Rru_A3009 0.31 -1.0 3,466,646 + Rru_A3009 0.35 +1.9 3,466,791 - Rru_A3009 0.46 -0.7 3,466,867 - Rru_A3009 0.52 +0.0 3,467,109 + Rru_A3009 0.70 +0.0 3,467,218 - Rru_A3009 0.78 +0.0 3,467,488 + +0.0 3,467,488 + +0.0 3,467,488 + +0.7 3,467,488 + -0.2 3,467,488 + -1.0 3,467,488 + -0.3 3,467,489 - +0.0 3,467,489 - +0.0 3,467,489 - +0.0 3,467,624 + Rru_A3010 0.13 +1.0 3,467,705 + Rru_A3010 0.23 +0.0 3,467,705 + Rru_A3010 0.23 +0.0 3,467,706 - Rru_A3010 0.23 +0.0 3,467,706 - Rru_A3010 0.23 -1.0 3,467,786 + Rru_A3010 0.33 +0.0 3,467,801 + Rru_A3010 0.35 +0.0 3,467,801 + Rru_A3010 0.35 +0.0 3,467,802 - Rru_A3010 0.35 +0.0 3,467,802 - Rru_A3010 0.35 +0.0 3,467,802 - Rru_A3010 0.35 -1.0 3,467,846 + Rru_A3010 0.41 +0.0 3,467,846 + Rru_A3010 0.41 +0.0 3,467,847 - Rru_A3010 0.41 +0.0 3,468,119 + Rru_A3010 0.75 +0.0 3,468,119 + Rru_A3010 0.75 -1.0 3,468,119 + Rru_A3010 0.75 +0.0 3,468,120 - Rru_A3010 0.75 +0.0 3,468,120 - Rru_A3010 0.75 -1.5 3,468,474 + +0.8 3,468,475 - +0.0 3,468,475 - +0.0 3,468,475 - +0.7 3,468,475 - +0.9
Or see this region's nucleotide sequence