Experiment: Succinic Acid; Light intensity 75 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1927 and Rru_A1928 are separated by 636 nucleotides Rru_A1928 and Rru_A1929 are separated by 123 nucleotides Rru_A1929 and Rru_A1930 overlap by 4 nucleotides
Rru_A1927: Rru_A1927 - Acetyl-CoA hydrolase (NCBI), at 2,231,609 to 2,233,114
_A1927
Rru_A1928: Rru_A1928 - Yeast 2-isopropylmalate synthase (NCBI), at 2,233,751 to 2,235,427
_A1928
Rru_A1929: Rru_A1929 - Phosphoadenosine phosphosulfate reductase (NCBI), at 2,235,551 to 2,236,279
_A1929
Rru_A1930: Rru_A1930 - Uncharacterized conserved protein UCP030820 (NCBI), at 2,236,276 to 2,236,812
_A1930
Position (kb)
2233
2234
2235
2236 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2232.760 kb on + strand, within Rru_A1927 at 2232.935 kb on - strand, within Rru_A1927 at 2233.111 kb on - strand at 2233.111 kb on - strand at 2233.242 kb on + strand at 2233.370 kb on + strand at 2233.381 kb on + strand at 2233.381 kb on + strand at 2233.382 kb on - strand at 2233.447 kb on + strand at 2233.447 kb on + strand at 2233.447 kb on + strand at 2233.447 kb on + strand at 2233.447 kb on + strand at 2233.447 kb on + strand at 2233.448 kb on - strand at 2233.448 kb on - strand at 2233.633 kb on + strand at 2233.634 kb on - strand at 2234.132 kb on + strand, within Rru_A1928 at 2234.133 kb on - strand, within Rru_A1928 at 2234.134 kb on + strand, within Rru_A1928 at 2234.134 kb on + strand, within Rru_A1928 at 2234.134 kb on + strand, within Rru_A1928 at 2234.134 kb on + strand, within Rru_A1928 at 2234.135 kb on - strand, within Rru_A1928 at 2234.135 kb on - strand, within Rru_A1928 at 2234.166 kb on + strand, within Rru_A1928 at 2234.167 kb on - strand, within Rru_A1928 at 2234.273 kb on + strand, within Rru_A1928 at 2234.273 kb on + strand, within Rru_A1928 at 2234.274 kb on - strand, within Rru_A1928 at 2234.309 kb on + strand, within Rru_A1928 at 2234.310 kb on - strand, within Rru_A1928 at 2234.417 kb on + strand, within Rru_A1928 at 2234.418 kb on - strand, within Rru_A1928 at 2234.418 kb on - strand, within Rru_A1928 at 2234.662 kb on + strand, within Rru_A1928 at 2234.695 kb on + strand, within Rru_A1928 at 2234.726 kb on + strand, within Rru_A1928 at 2234.727 kb on - strand, within Rru_A1928 at 2234.747 kb on - strand, within Rru_A1928 at 2234.747 kb on - strand, within Rru_A1928 at 2234.834 kb on + strand, within Rru_A1928 at 2234.835 kb on - strand, within Rru_A1928 at 2234.870 kb on + strand, within Rru_A1928 at 2234.870 kb on + strand, within Rru_A1928 at 2234.870 kb on + strand, within Rru_A1928 at 2234.870 kb on + strand, within Rru_A1928 at 2234.870 kb on + strand, within Rru_A1928 at 2234.870 kb on + strand, within Rru_A1928 at 2234.871 kb on - strand, within Rru_A1928 at 2234.871 kb on - strand, within Rru_A1928 at 2234.871 kb on - strand, within Rru_A1928 at 2234.871 kb on - strand, within Rru_A1928 at 2234.871 kb on - strand, within Rru_A1928 at 2234.923 kb on + strand, within Rru_A1928 at 2234.924 kb on - strand, within Rru_A1928 at 2235.060 kb on - strand, within Rru_A1928 at 2235.082 kb on + strand, within Rru_A1928 at 2235.082 kb on + strand, within Rru_A1928 at 2235.082 kb on + strand, within Rru_A1928 at 2235.083 kb on - strand, within Rru_A1928 at 2235.083 kb on - strand, within Rru_A1928 at 2235.083 kb on - strand, within Rru_A1928 at 2235.083 kb on - strand, within Rru_A1928 at 2235.260 kb on + strand at 2235.344 kb on + strand at 2235.425 kb on + strand at 2235.425 kb on + strand at 2235.425 kb on + strand at 2235.426 kb on - strand at 2235.426 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 75 uM remove 2,232,760 + Rru_A1927 0.76 +1.6 2,232,935 - Rru_A1927 0.88 +0.7 2,233,111 - +0.6 2,233,111 - +1.4 2,233,242 + -1.1 2,233,370 + +1.8 2,233,381 + +0.0 2,233,381 + -0.1 2,233,382 - -0.1 2,233,447 + +0.1 2,233,447 + -0.1 2,233,447 + -0.1 2,233,447 + +0.5 2,233,447 + -0.4 2,233,447 + -0.1 2,233,448 - -1.4 2,233,448 - -3.8 2,233,633 + +0.9 2,233,634 - +0.9 2,234,132 + Rru_A1928 0.23 -2.9 2,234,133 - Rru_A1928 0.23 -0.1 2,234,134 + Rru_A1928 0.23 -1.7 2,234,134 + Rru_A1928 0.23 +0.1 2,234,134 + Rru_A1928 0.23 -1.0 2,234,134 + Rru_A1928 0.23 -1.7 2,234,135 - Rru_A1928 0.23 -1.1 2,234,135 - Rru_A1928 0.23 -2.2 2,234,166 + Rru_A1928 0.25 -1.1 2,234,167 - Rru_A1928 0.25 -1.2 2,234,273 + Rru_A1928 0.31 -1.1 2,234,273 + Rru_A1928 0.31 -0.1 2,234,274 - Rru_A1928 0.31 -2.0 2,234,309 + Rru_A1928 0.33 -1.2 2,234,310 - Rru_A1928 0.33 -2.8 2,234,417 + Rru_A1928 0.40 -0.1 2,234,418 - Rru_A1928 0.40 -0.1 2,234,418 - Rru_A1928 0.40 -2.0 2,234,662 + Rru_A1928 0.54 +0.2 2,234,695 + Rru_A1928 0.56 -2.8 2,234,726 + Rru_A1928 0.58 -2.0 2,234,727 - Rru_A1928 0.58 -0.5 2,234,747 - Rru_A1928 0.59 -0.1 2,234,747 - Rru_A1928 0.59 -2.4 2,234,834 + Rru_A1928 0.65 -0.1 2,234,835 - Rru_A1928 0.65 -0.1 2,234,870 + Rru_A1928 0.67 -0.1 2,234,870 + Rru_A1928 0.67 -3.3 2,234,870 + Rru_A1928 0.67 -0.1 2,234,870 + Rru_A1928 0.67 -0.1 2,234,870 + Rru_A1928 0.67 -0.1 2,234,870 + Rru_A1928 0.67 -3.3 2,234,871 - Rru_A1928 0.67 -0.1 2,234,871 - Rru_A1928 0.67 -0.1 2,234,871 - Rru_A1928 0.67 -0.6 2,234,871 - Rru_A1928 0.67 -0.1 2,234,871 - Rru_A1928 0.67 -1.6 2,234,923 + Rru_A1928 0.70 -3.2 2,234,924 - Rru_A1928 0.70 -0.3 2,235,060 - Rru_A1928 0.78 -1.1 2,235,082 + Rru_A1928 0.79 -0.1 2,235,082 + Rru_A1928 0.79 -0.1 2,235,082 + Rru_A1928 0.79 -0.8 2,235,083 - Rru_A1928 0.79 -2.3 2,235,083 - Rru_A1928 0.79 -2.0 2,235,083 - Rru_A1928 0.79 -2.1 2,235,083 - Rru_A1928 0.79 -2.1 2,235,260 + -0.1 2,235,344 + -0.1 2,235,425 + +2.5 2,235,425 + +1.9 2,235,425 + +2.0 2,235,426 - -0.1 2,235,426 - +2.2
Or see this region's nucleotide sequence