Experiment: Succinic Acid; Light intensity 75 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1320 and Rru_A1321 are separated by 101 nucleotides Rru_A1321 and Rru_A1322 are separated by 32 nucleotides Rru_A1322 and Rru_A1323 are separated by 17 nucleotides Rru_A1323 and Rru_A1324 are separated by 13 nucleotides
Rru_A1320: Rru_A1320 - Na+-transporting methylmalonyl-CoA/oxaloacetate decarboxylase, beta subunit (NCBI), at 1,558,503 to 1,559,714
_A1320
Rru_A1321: Rru_A1321 - Biotin--acetyl-CoA-carboxylase ligase (NCBI), at 1,559,816 to 1,560,649
_A1321
Rru_A1322: Rru_A1322 - alpha subunit of malonate decarboxylase (NCBI), at 1,560,682 to 1,562,355
_A1322
Rru_A1323: Rru_A1323 - Malonate decarboxylase delta subunit (NCBI), at 1,562,373 to 1,562,690
_A1323
Rru_A1324: Rru_A1324 - beta subunit of malonate decarboxylase (NCBI), at 1,562,704 to 1,563,660
_A1324
Position (kb)
1560
1561
1562
1563 Strain fitness (log2 ratio)
-2
-1
0
1 at 1559.720 kb on - strand at 1559.720 kb on - strand at 1559.804 kb on - strand at 1560.195 kb on + strand, within Rru_A1321 at 1560.296 kb on - strand, within Rru_A1321 at 1560.696 kb on + strand at 1560.730 kb on - strand at 1560.894 kb on + strand, within Rru_A1322 at 1560.895 kb on - strand, within Rru_A1322 at 1561.081 kb on + strand, within Rru_A1322 at 1561.082 kb on - strand, within Rru_A1322 at 1561.082 kb on - strand, within Rru_A1322 at 1561.103 kb on - strand, within Rru_A1322 at 1561.144 kb on + strand, within Rru_A1322 at 1561.145 kb on - strand, within Rru_A1322 at 1561.146 kb on + strand, within Rru_A1322 at 1561.147 kb on - strand, within Rru_A1322 at 1561.147 kb on - strand, within Rru_A1322 at 1561.147 kb on - strand, within Rru_A1322 at 1561.147 kb on - strand, within Rru_A1322 at 1561.171 kb on + strand, within Rru_A1322 at 1561.171 kb on + strand, within Rru_A1322 at 1561.171 kb on + strand, within Rru_A1322 at 1561.171 kb on + strand, within Rru_A1322 at 1561.171 kb on + strand, within Rru_A1322 at 1561.172 kb on - strand, within Rru_A1322 at 1561.172 kb on - strand, within Rru_A1322 at 1561.417 kb on + strand, within Rru_A1322 at 1561.418 kb on - strand, within Rru_A1322 at 1561.426 kb on + strand, within Rru_A1322 at 1561.495 kb on + strand, within Rru_A1322 at 1561.496 kb on - strand, within Rru_A1322 at 1561.496 kb on - strand, within Rru_A1322 at 1561.536 kb on + strand, within Rru_A1322 at 1561.536 kb on + strand, within Rru_A1322 at 1561.536 kb on + strand, within Rru_A1322 at 1561.536 kb on + strand, within Rru_A1322 at 1561.537 kb on - strand, within Rru_A1322 at 1561.537 kb on - strand, within Rru_A1322 at 1561.633 kb on + strand, within Rru_A1322 at 1561.633 kb on + strand, within Rru_A1322 at 1561.634 kb on - strand, within Rru_A1322 at 1561.634 kb on - strand, within Rru_A1322 at 1561.720 kb on + strand, within Rru_A1322 at 1561.720 kb on + strand, within Rru_A1322 at 1561.720 kb on + strand, within Rru_A1322 at 1561.721 kb on - strand, within Rru_A1322 at 1562.054 kb on - strand, within Rru_A1322 at 1562.054 kb on - strand, within Rru_A1322 at 1562.098 kb on + strand, within Rru_A1322 at 1562.098 kb on + strand, within Rru_A1322 at 1562.098 kb on + strand, within Rru_A1322 at 1562.098 kb on + strand, within Rru_A1322 at 1562.099 kb on - strand, within Rru_A1322 at 1562.323 kb on + strand at 1562.323 kb on + strand at 1562.324 kb on - strand at 1562.324 kb on - strand at 1562.356 kb on + strand at 1562.356 kb on + strand at 1562.688 kb on + strand at 1562.688 kb on + strand at 1562.688 kb on + strand at 1562.704 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 75 uM remove 1,559,720 - -0.5 1,559,720 - +0.5 1,559,804 - -0.1 1,560,195 + Rru_A1321 0.45 -0.7 1,560,296 - Rru_A1321 0.58 +0.7 1,560,696 + +1.2 1,560,730 - +1.0 1,560,894 + Rru_A1322 0.13 -0.5 1,560,895 - Rru_A1322 0.13 +0.8 1,561,081 + Rru_A1322 0.24 +1.2 1,561,082 - Rru_A1322 0.24 +0.3 1,561,082 - Rru_A1322 0.24 -0.1 1,561,103 - Rru_A1322 0.25 -0.1 1,561,144 + Rru_A1322 0.28 -0.1 1,561,145 - Rru_A1322 0.28 -0.1 1,561,146 + Rru_A1322 0.28 -0.1 1,561,147 - Rru_A1322 0.28 -0.1 1,561,147 - Rru_A1322 0.28 -0.1 1,561,147 - Rru_A1322 0.28 +0.5 1,561,147 - Rru_A1322 0.28 +1.5 1,561,171 + Rru_A1322 0.29 +0.6 1,561,171 + Rru_A1322 0.29 -0.1 1,561,171 + Rru_A1322 0.29 -0.9 1,561,171 + Rru_A1322 0.29 +0.4 1,561,171 + Rru_A1322 0.29 -0.0 1,561,172 - Rru_A1322 0.29 +0.1 1,561,172 - Rru_A1322 0.29 -0.1 1,561,417 + Rru_A1322 0.44 +0.8 1,561,418 - Rru_A1322 0.44 -0.1 1,561,426 + Rru_A1322 0.44 -1.1 1,561,495 + Rru_A1322 0.49 +0.6 1,561,496 - Rru_A1322 0.49 +0.3 1,561,496 - Rru_A1322 0.49 +0.9 1,561,536 + Rru_A1322 0.51 -1.1 1,561,536 + Rru_A1322 0.51 +0.1 1,561,536 + Rru_A1322 0.51 +0.7 1,561,536 + Rru_A1322 0.51 -0.7 1,561,537 - Rru_A1322 0.51 -0.1 1,561,537 - Rru_A1322 0.51 +1.1 1,561,633 + Rru_A1322 0.57 +0.4 1,561,633 + Rru_A1322 0.57 -0.1 1,561,634 - Rru_A1322 0.57 -0.1 1,561,634 - Rru_A1322 0.57 +0.5 1,561,720 + Rru_A1322 0.62 -0.1 1,561,720 + Rru_A1322 0.62 +1.0 1,561,720 + Rru_A1322 0.62 -0.1 1,561,721 - Rru_A1322 0.62 -0.1 1,562,054 - Rru_A1322 0.82 -0.1 1,562,054 - Rru_A1322 0.82 +0.9 1,562,098 + Rru_A1322 0.85 -2.7 1,562,098 + Rru_A1322 0.85 +0.9 1,562,098 + Rru_A1322 0.85 -0.1 1,562,098 + Rru_A1322 0.85 -0.1 1,562,099 - Rru_A1322 0.85 +0.9 1,562,323 + -0.1 1,562,323 + -1.5 1,562,324 - +0.8 1,562,324 - +0.6 1,562,356 + -1.4 1,562,356 + -1.3 1,562,688 + -1.0 1,562,688 + -0.7 1,562,688 + -0.1 1,562,704 - -1.1
Or see this region's nucleotide sequence