Experiment: Succinic Acid; Light intensity 75 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1299 and Rru_A1300 are separated by 5 nucleotides Rru_A1300 and Rru_A1301 are separated by 16 nucleotides Rru_A1301 and Rru_A1302 overlap by 4 nucleotides
Rru_A1299: Rru_A1299 - FAD linked oxidase-like (NCBI), at 1,532,308 to 1,533,678
_A1299
Rru_A1300: Rru_A1300 - N-formylglutamate deformylase (NCBI), at 1,533,684 to 1,534,478
_A1300
Rru_A1301: Rru_A1301 - Histidine ammonia-lyase (NCBI), at 1,534,495 to 1,536,039
_A1301
Rru_A1302: Rru_A1302 - Formiminoglutamate deiminase (NCBI), at 1,536,036 to 1,537,403
_A1302
Position (kb)
1534
1535
1536
1537 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1533.676 kb on + strand at 1533.685 kb on + strand at 1533.686 kb on - strand at 1533.686 kb on - strand at 1533.686 kb on - strand at 1533.766 kb on + strand, within Rru_A1300 at 1533.766 kb on + strand, within Rru_A1300 at 1533.767 kb on - strand, within Rru_A1300 at 1533.782 kb on + strand, within Rru_A1300 at 1533.783 kb on - strand, within Rru_A1300 at 1533.783 kb on - strand, within Rru_A1300 at 1533.784 kb on + strand, within Rru_A1300 at 1533.784 kb on + strand, within Rru_A1300 at 1533.785 kb on - strand, within Rru_A1300 at 1533.951 kb on + strand, within Rru_A1300 at 1534.043 kb on - strand, within Rru_A1300 at 1534.172 kb on - strand, within Rru_A1300 at 1534.466 kb on - strand at 1534.466 kb on - strand at 1534.466 kb on - strand at 1534.466 kb on - strand at 1534.592 kb on + strand at 1534.592 kb on + strand at 1535.095 kb on - strand, within Rru_A1301 at 1535.192 kb on + strand, within Rru_A1301 at 1535.193 kb on - strand, within Rru_A1301 at 1535.193 kb on - strand, within Rru_A1301 at 1535.462 kb on - strand, within Rru_A1301 at 1535.462 kb on - strand, within Rru_A1301 at 1535.542 kb on - strand, within Rru_A1301 at 1535.642 kb on - strand, within Rru_A1301 at 1535.876 kb on + strand, within Rru_A1301 at 1535.876 kb on + strand, within Rru_A1301 at 1535.876 kb on + strand, within Rru_A1301 at 1535.877 kb on - strand, within Rru_A1301 at 1535.992 kb on + strand at 1535.992 kb on + strand at 1535.992 kb on + strand at 1535.992 kb on + strand at 1535.992 kb on + strand at 1535.992 kb on + strand at 1535.992 kb on + strand at 1535.993 kb on - strand at 1535.993 kb on - strand at 1535.993 kb on - strand at 1535.993 kb on - strand at 1535.993 kb on - strand at 1536.391 kb on + strand, within Rru_A1302 at 1536.391 kb on + strand, within Rru_A1302 at 1536.431 kb on + strand, within Rru_A1302 at 1536.431 kb on + strand, within Rru_A1302 at 1536.432 kb on - strand, within Rru_A1302 at 1536.432 kb on - strand, within Rru_A1302 at 1536.432 kb on - strand, within Rru_A1302 at 1536.432 kb on - strand, within Rru_A1302 at 1536.432 kb on - strand, within Rru_A1302 at 1536.432 kb on - strand, within Rru_A1302 at 1536.432 kb on - strand, within Rru_A1302 at 1536.432 kb on - strand, within Rru_A1302 at 1536.678 kb on - strand, within Rru_A1302 at 1536.701 kb on + strand, within Rru_A1302 at 1536.702 kb on - strand, within Rru_A1302 at 1536.922 kb on + strand, within Rru_A1302 at 1536.923 kb on - strand, within Rru_A1302 at 1536.923 kb on - strand, within Rru_A1302 at 1536.923 kb on - strand, within Rru_A1302 at 1537.037 kb on - strand, within Rru_A1302 at 1537.037 kb on - strand, within Rru_A1302
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 75 uM remove 1,533,676 + +1.0 1,533,685 + +0.3 1,533,686 - +0.7 1,533,686 - -0.4 1,533,686 - +1.6 1,533,766 + Rru_A1300 0.10 -0.1 1,533,766 + Rru_A1300 0.10 -1.1 1,533,767 - Rru_A1300 0.10 +1.6 1,533,782 + Rru_A1300 0.12 +0.2 1,533,783 - Rru_A1300 0.12 -0.1 1,533,783 - Rru_A1300 0.12 +0.5 1,533,784 + Rru_A1300 0.13 +1.0 1,533,784 + Rru_A1300 0.13 +1.2 1,533,785 - Rru_A1300 0.13 -0.1 1,533,951 + Rru_A1300 0.34 -1.3 1,534,043 - Rru_A1300 0.45 -1.9 1,534,172 - Rru_A1300 0.61 +0.5 1,534,466 - -0.1 1,534,466 - +2.0 1,534,466 - -0.1 1,534,466 - -1.1 1,534,592 + -0.1 1,534,592 + -0.1 1,535,095 - Rru_A1301 0.39 -0.1 1,535,192 + Rru_A1301 0.45 +0.9 1,535,193 - Rru_A1301 0.45 -0.1 1,535,193 - Rru_A1301 0.45 +0.9 1,535,462 - Rru_A1301 0.63 -0.2 1,535,462 - Rru_A1301 0.63 -0.1 1,535,542 - Rru_A1301 0.68 -0.1 1,535,642 - Rru_A1301 0.74 +0.3 1,535,876 + Rru_A1301 0.89 -0.1 1,535,876 + Rru_A1301 0.89 +0.5 1,535,876 + Rru_A1301 0.89 +2.5 1,535,877 - Rru_A1301 0.89 -0.1 1,535,992 + -0.1 1,535,992 + -0.5 1,535,992 + +0.9 1,535,992 + +0.1 1,535,992 + +0.8 1,535,992 + +0.5 1,535,992 + -0.1 1,535,993 - -0.1 1,535,993 - -0.1 1,535,993 - -0.1 1,535,993 - -0.1 1,535,993 - +0.2 1,536,391 + Rru_A1302 0.26 -0.1 1,536,391 + Rru_A1302 0.26 +1.4 1,536,431 + Rru_A1302 0.29 +1.7 1,536,431 + Rru_A1302 0.29 -0.6 1,536,432 - Rru_A1302 0.29 -0.1 1,536,432 - Rru_A1302 0.29 +0.1 1,536,432 - Rru_A1302 0.29 +2.5 1,536,432 - Rru_A1302 0.29 +0.6 1,536,432 - Rru_A1302 0.29 -0.1 1,536,432 - Rru_A1302 0.29 +0.5 1,536,432 - Rru_A1302 0.29 +0.7 1,536,432 - Rru_A1302 0.29 -0.1 1,536,678 - Rru_A1302 0.47 +0.9 1,536,701 + Rru_A1302 0.49 +1.6 1,536,702 - Rru_A1302 0.49 -0.1 1,536,922 + Rru_A1302 0.65 -0.1 1,536,923 - Rru_A1302 0.65 -2.1 1,536,923 - Rru_A1302 0.65 +0.6 1,536,923 - Rru_A1302 0.65 +0.5 1,537,037 - Rru_A1302 0.73 -0.1 1,537,037 - Rru_A1302 0.73 +1.1
Or see this region's nucleotide sequence