Experiment: Succinic Acid; Light intensity 75 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0819 and Rru_A0820 are separated by 18 nucleotides Rru_A0820 and Rru_A0821 overlap by 1 nucleotides Rru_A0821 and Rru_A0822 overlap by 4 nucleotides Rru_A0822 and Rru_A0823 are separated by 218 nucleotides
Rru_A0819: Rru_A0819 - Asparagine synthase (NCBI), at 974,936 to 976,897
_A0819
Rru_A0820: Rru_A0820 - HPr kinase (NCBI), at 976,916 to 977,821
_A0820
Rru_A0821: Rru_A0821 - hypothetical protein (NCBI), at 977,821 to 978,279
_A0821
Rru_A0822: Rru_A0822 - hypothetical protein (NCBI), at 978,276 to 978,554
_A0822
Rru_A0823: Rru_A0823 - Cupin region (NCBI), at 978,773 to 979,678
_A0823
Position (kb) 977
978
979 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 976.826 kb on + strand at 976.827 kb on - strand at 977.001 kb on - strand at 977.068 kb on - strand, within Rru_A0820 at 977.218 kb on + strand, within Rru_A0820 at 977.218 kb on + strand, within Rru_A0820 at 977.218 kb on + strand, within Rru_A0820 at 977.219 kb on - strand, within Rru_A0820 at 977.219 kb on - strand, within Rru_A0820 at 977.219 kb on - strand, within Rru_A0820 at 977.219 kb on - strand, within Rru_A0820 at 977.219 kb on - strand, within Rru_A0820 at 977.426 kb on - strand, within Rru_A0820 at 977.635 kb on + strand, within Rru_A0820 at 977.636 kb on - strand, within Rru_A0820 at 977.636 kb on - strand, within Rru_A0820 at 977.843 kb on + strand at 977.843 kb on + strand at 977.964 kb on + strand, within Rru_A0821 at 977.964 kb on + strand, within Rru_A0821 at 977.964 kb on + strand, within Rru_A0821 at 977.965 kb on - strand, within Rru_A0821 at 977.965 kb on - strand, within Rru_A0821 at 978.364 kb on + strand, within Rru_A0822 at 978.365 kb on - strand, within Rru_A0822 at 978.428 kb on + strand, within Rru_A0822 at 978.428 kb on + strand, within Rru_A0822 at 978.429 kb on - strand, within Rru_A0822 at 978.457 kb on + strand, within Rru_A0822 at 978.457 kb on + strand, within Rru_A0822 at 978.458 kb on - strand, within Rru_A0822 at 978.458 kb on - strand, within Rru_A0822 at 978.609 kb on + strand at 978.609 kb on + strand at 978.610 kb on - strand at 978.610 kb on - strand at 978.635 kb on + strand at 978.687 kb on - strand at 978.750 kb on + strand at 978.859 kb on - strand at 978.890 kb on - strand, within Rru_A0823 at 978.930 kb on - strand, within Rru_A0823 at 978.932 kb on - strand, within Rru_A0823 at 978.932 kb on - strand, within Rru_A0823 at 979.095 kb on - strand, within Rru_A0823 at 979.196 kb on + strand, within Rru_A0823 at 979.197 kb on - strand, within Rru_A0823
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 75 uM remove 976,826 + -1.0 976,827 - -0.0 977,001 - +0.3 977,068 - Rru_A0820 0.17 +1.2 977,218 + Rru_A0820 0.33 +0.8 977,218 + Rru_A0820 0.33 +0.7 977,218 + Rru_A0820 0.33 -2.8 977,219 - Rru_A0820 0.33 +2.0 977,219 - Rru_A0820 0.33 -0.0 977,219 - Rru_A0820 0.33 -0.0 977,219 - Rru_A0820 0.33 -0.0 977,219 - Rru_A0820 0.33 +0.6 977,426 - Rru_A0820 0.56 +0.7 977,635 + Rru_A0820 0.79 +0.7 977,636 - Rru_A0820 0.79 +1.0 977,636 - Rru_A0820 0.79 +0.2 977,843 + -0.0 977,843 + +0.7 977,964 + Rru_A0821 0.31 +0.8 977,964 + Rru_A0821 0.31 +0.3 977,964 + Rru_A0821 0.31 +0.6 977,965 - Rru_A0821 0.31 +0.2 977,965 - Rru_A0821 0.31 +0.9 978,364 + Rru_A0822 0.32 +1.2 978,365 - Rru_A0822 0.32 -1.0 978,428 + Rru_A0822 0.54 +1.2 978,428 + Rru_A0822 0.54 +1.0 978,429 - Rru_A0822 0.55 +0.6 978,457 + Rru_A0822 0.65 -0.0 978,457 + Rru_A0822 0.65 +0.8 978,458 - Rru_A0822 0.65 -0.0 978,458 - Rru_A0822 0.65 -0.0 978,609 + +1.4 978,609 + -0.0 978,610 - +0.2 978,610 - +1.4 978,635 + -0.0 978,687 - -0.0 978,750 + -0.0 978,859 - +1.1 978,890 - Rru_A0823 0.13 +0.9 978,930 - Rru_A0823 0.17 +0.6 978,932 - Rru_A0823 0.18 +1.2 978,932 - Rru_A0823 0.18 -0.0 979,095 - Rru_A0823 0.36 +0.8 979,196 + Rru_A0823 0.47 -0.0 979,197 - Rru_A0823 0.47 -2.0
Or see this region's nucleotide sequence