Experiment: Succinic Acid; Light intensity 75 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0004 and Rru_A0005 are separated by 413 nucleotides Rru_A0005 and Rru_A0006 overlap by 14 nucleotides Rru_A0006 and Rru_A0007 are separated by 105 nucleotides Rru_A0007 and Rru_A0008 are separated by 103 nucleotides
Rru_A0004: Rru_A0004 - DNA gyrase, B subunit (NCBI), at 4,362 to 6,770
_A0004
Rru_A0005: Rru_A0005 - killer suppression protein HigA, putative (NCBI), at 7,184 to 7,528
_A0005
Rru_A0006: Rru_A0006 - Plasmid maintenance system antidote protein (NCBI), at 7,515 to 8,657
_A0006
Rru_A0007: Rru_A0007 - hypothetical protein (NCBI), at 8,763 to 9,104
_A0007
Rru_A0008: Rru_A0008 - hypothetical protein (NCBI), at 9,208 to 9,414
_A0008
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6.852 kb on + strand at 6.853 kb on - strand at 6.853 kb on - strand at 6.853 kb on - strand at 6.988 kb on - strand at 7.002 kb on + strand at 7.008 kb on - strand at 7.095 kb on - strand at 7.192 kb on + strand at 7.196 kb on + strand at 7.197 kb on - strand at 7.201 kb on + strand at 7.202 kb on - strand at 7.202 kb on - strand at 7.245 kb on + strand, within Rru_A0005 at 7.246 kb on - strand, within Rru_A0005 at 7.354 kb on + strand, within Rru_A0005 at 7.354 kb on + strand, within Rru_A0005 at 7.354 kb on + strand, within Rru_A0005 at 7.411 kb on + strand, within Rru_A0005 at 7.411 kb on + strand, within Rru_A0005 at 7.411 kb on + strand, within Rru_A0005 at 7.411 kb on + strand, within Rru_A0005 at 7.412 kb on - strand, within Rru_A0005 at 7.412 kb on - strand, within Rru_A0005 at 7.471 kb on + strand, within Rru_A0005 at 7.471 kb on + strand, within Rru_A0005 at 7.472 kb on - strand, within Rru_A0005 at 7.472 kb on - strand, within Rru_A0005 at 7.472 kb on - strand, within Rru_A0005 at 7.472 kb on - strand, within Rru_A0005 at 7.472 kb on - strand, within Rru_A0005 at 7.472 kb on - strand, within Rru_A0005 at 7.511 kb on + strand at 7.512 kb on - strand at 8.099 kb on + strand, within Rru_A0006 at 8.099 kb on + strand, within Rru_A0006 at 8.099 kb on + strand, within Rru_A0006 at 8.279 kb on + strand, within Rru_A0006 at 8.280 kb on - strand, within Rru_A0006 at 8.580 kb on + strand at 8.655 kb on + strand at 8.656 kb on - strand at 8.700 kb on - strand at 9.233 kb on + strand, within Rru_A0008 at 9.233 kb on + strand, within Rru_A0008 at 9.233 kb on + strand, within Rru_A0008 at 9.234 kb on - strand, within Rru_A0008 at 9.234 kb on - strand, within Rru_A0008 at 9.234 kb on - strand, within Rru_A0008 at 9.234 kb on - strand, within Rru_A0008 at 9.275 kb on + strand, within Rru_A0008 at 9.275 kb on + strand, within Rru_A0008 at 9.275 kb on + strand, within Rru_A0008 at 9.275 kb on + strand, within Rru_A0008 at 9.275 kb on + strand, within Rru_A0008 at 9.276 kb on - strand, within Rru_A0008 at 9.375 kb on + strand, within Rru_A0008 at 9.393 kb on + strand, within Rru_A0008 at 9.393 kb on + strand, within Rru_A0008 at 9.414 kb on + strand at 9.415 kb on - strand at 9.499 kb on + strand at 9.499 kb on + strand at 9.500 kb on - strand at 9.500 kb on - strand at 9.505 kb on + strand at 9.510 kb on + strand at 9.510 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 75 uM remove 6,852 + -1.0 6,853 - +0.0 6,853 - +1.9 6,853 - +0.0 6,988 - +1.4 7,002 + -1.5 7,008 - +1.7 7,095 - +0.8 7,192 + +0.0 7,196 + +1.0 7,197 - +0.0 7,201 + +0.9 7,202 - +1.4 7,202 - +0.0 7,245 + Rru_A0005 0.18 +1.3 7,246 - Rru_A0005 0.18 +0.0 7,354 + Rru_A0005 0.49 +0.4 7,354 + Rru_A0005 0.49 +0.0 7,354 + Rru_A0005 0.49 -1.5 7,411 + Rru_A0005 0.66 +1.2 7,411 + Rru_A0005 0.66 +1.5 7,411 + Rru_A0005 0.66 +0.5 7,411 + Rru_A0005 0.66 -2.0 7,412 - Rru_A0005 0.66 +0.6 7,412 - Rru_A0005 0.66 +0.0 7,471 + Rru_A0005 0.83 +0.0 7,471 + Rru_A0005 0.83 +0.6 7,472 - Rru_A0005 0.83 +0.0 7,472 - Rru_A0005 0.83 -0.8 7,472 - Rru_A0005 0.83 +0.0 7,472 - Rru_A0005 0.83 -1.0 7,472 - Rru_A0005 0.83 +1.1 7,472 - Rru_A0005 0.83 +0.0 7,511 + +0.0 7,512 - +1.0 8,099 + Rru_A0006 0.51 +0.0 8,099 + Rru_A0006 0.51 -0.8 8,099 + Rru_A0006 0.51 +0.0 8,279 + Rru_A0006 0.67 -0.4 8,280 - Rru_A0006 0.67 +0.0 8,580 + +0.5 8,655 + +1.8 8,656 - +0.0 8,700 - -1.0 9,233 + Rru_A0008 0.12 -0.2 9,233 + Rru_A0008 0.12 -0.3 9,233 + Rru_A0008 0.12 +2.4 9,234 - Rru_A0008 0.13 -0.4 9,234 - Rru_A0008 0.13 +1.0 9,234 - Rru_A0008 0.13 +0.0 9,234 - Rru_A0008 0.13 -1.5 9,275 + Rru_A0008 0.32 +0.0 9,275 + Rru_A0008 0.32 +1.8 9,275 + Rru_A0008 0.32 +1.4 9,275 + Rru_A0008 0.32 +0.2 9,275 + Rru_A0008 0.32 +1.3 9,276 - Rru_A0008 0.33 +1.2 9,375 + Rru_A0008 0.81 +0.0 9,393 + Rru_A0008 0.89 +0.0 9,393 + Rru_A0008 0.89 +0.0 9,414 + +1.2 9,415 - +0.0 9,499 + +0.6 9,499 + +0.7 9,500 - +0.8 9,500 - +0.0 9,505 + +0.9 9,510 + -0.4 9,510 + +1.2
Or see this region's nucleotide sequence