Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25240 and AO356_25245 overlap by 4 nucleotides AO356_25245 and AO356_25250 are separated by 136 nucleotides AO356_25250 and AO356_25255 overlap by 13 nucleotides AO356_25255 and AO356_25260 are separated by 22 nucleotides
AO356_25240: AO356_25240 - oxidoreductase, at 5,715,111 to 5,715,272
_25240
AO356_25245: AO356_25245 - anaerobic ribonucleoside-triphosphate reductase activating protein, at 5,715,269 to 5,715,964
_25245
AO356_25250: AO356_25250 - hypothetical protein, at 5,716,101 to 5,717,468
_25250
AO356_25255: AO356_25255 - FTR1 family iron permease, at 5,717,456 to 5,718,310
_25255
AO356_25260: AO356_25260 - iron transporter, at 5,718,333 to 5,718,677
_25260
Position (kb) 5716
5717
5718 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5715.740 kb on + strand, within AO356_25245 at 5715.741 kb on - strand, within AO356_25245 at 5715.958 kb on + strand at 5715.958 kb on + strand at 5715.959 kb on - strand at 5715.959 kb on - strand at 5715.959 kb on - strand at 5716.094 kb on + strand at 5716.095 kb on - strand at 5716.095 kb on - strand at 5716.102 kb on + strand at 5716.303 kb on + strand, within AO356_25250 at 5716.304 kb on - strand, within AO356_25250 at 5716.390 kb on + strand, within AO356_25250 at 5716.391 kb on - strand, within AO356_25250 at 5716.391 kb on - strand, within AO356_25250 at 5716.905 kb on + strand, within AO356_25250 at 5717.071 kb on + strand, within AO356_25250 at 5717.254 kb on - strand, within AO356_25250 at 5717.371 kb on + strand at 5717.531 kb on + strand at 5717.623 kb on + strand, within AO356_25255 at 5717.623 kb on + strand, within AO356_25255 at 5717.656 kb on - strand, within AO356_25255 at 5717.656 kb on - strand, within AO356_25255 at 5718.155 kb on + strand, within AO356_25255 at 5718.195 kb on - strand, within AO356_25255 at 5718.195 kb on - strand, within AO356_25255 at 5718.195 kb on - strand, within AO356_25255 at 5718.292 kb on + strand at 5718.292 kb on + strand at 5718.293 kb on - strand at 5718.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,715,740 + AO356_25245 0.68 +1.3 5,715,741 - AO356_25245 0.68 +0.7 5,715,958 + +0.4 5,715,958 + -0.0 5,715,959 - -0.1 5,715,959 - +1.9 5,715,959 - +1.0 5,716,094 + +0.7 5,716,095 - +0.9 5,716,095 - +2.5 5,716,102 + -0.1 5,716,303 + AO356_25250 0.15 -1.5 5,716,304 - AO356_25250 0.15 +2.3 5,716,390 + AO356_25250 0.21 -1.3 5,716,391 - AO356_25250 0.21 +3.0 5,716,391 - AO356_25250 0.21 +0.8 5,716,905 + AO356_25250 0.59 +0.7 5,717,071 + AO356_25250 0.71 +0.5 5,717,254 - AO356_25250 0.84 +0.9 5,717,371 + +0.6 5,717,531 + +1.3 5,717,623 + AO356_25255 0.20 +1.1 5,717,623 + AO356_25255 0.20 +0.4 5,717,656 - AO356_25255 0.23 -0.6 5,717,656 - AO356_25255 0.23 +1.1 5,718,155 + AO356_25255 0.82 -0.1 5,718,195 - AO356_25255 0.86 +0.6 5,718,195 - AO356_25255 0.86 +1.4 5,718,195 - AO356_25255 0.86 +0.2 5,718,292 + +1.9 5,718,292 + -1.8 5,718,293 - -2.0 5,718,293 - +0.1
Or see this region's nucleotide sequence