Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25025 and AO356_25030 overlap by 4 nucleotides AO356_25030 and AO356_25035 are separated by 40 nucleotides AO356_25035 and AO356_25040 are separated by 114 nucleotides AO356_25040 and AO356_25045 overlap by 26 nucleotides
AO356_25025: AO356_25025 - cytochrome C oxidase Cbb3, at 5,670,179 to 5,671,675
_25025
AO356_25030: AO356_25030 - uroporphyrin-III methyltransferase, at 5,671,672 to 5,672,514
_25030
AO356_25035: AO356_25035 - heme d1 biosynthesis radical SAM protein NirJ, at 5,672,555 to 5,673,733
_25035
AO356_25040: AO356_25040 - nitrite reductase, at 5,673,848 to 5,674,417
_25040
AO356_25045: AO356_25045 - protein nirG, at 5,674,392 to 5,674,835
_25045
Position (kb) 5672
5673
5674 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5671.581 kb on + strand at 5671.581 kb on + strand at 5671.581 kb on + strand at 5671.581 kb on + strand at 5671.582 kb on - strand at 5671.961 kb on + strand, within AO356_25030 at 5671.962 kb on - strand, within AO356_25030 at 5671.962 kb on - strand, within AO356_25030 at 5671.962 kb on - strand, within AO356_25030 at 5671.962 kb on - strand, within AO356_25030 at 5672.093 kb on + strand, within AO356_25030 at 5672.162 kb on + strand, within AO356_25030 at 5672.162 kb on + strand, within AO356_25030 at 5672.163 kb on - strand, within AO356_25030 at 5672.309 kb on + strand, within AO356_25030 at 5672.829 kb on + strand, within AO356_25035 at 5672.829 kb on + strand, within AO356_25035 at 5672.829 kb on + strand, within AO356_25035 at 5672.830 kb on - strand, within AO356_25035 at 5672.830 kb on - strand, within AO356_25035 at 5672.928 kb on + strand, within AO356_25035 at 5672.928 kb on + strand, within AO356_25035 at 5673.120 kb on + strand, within AO356_25035 at 5673.120 kb on + strand, within AO356_25035 at 5673.121 kb on - strand, within AO356_25035 at 5673.121 kb on - strand, within AO356_25035 at 5673.138 kb on + strand, within AO356_25035 at 5673.138 kb on + strand, within AO356_25035 at 5673.139 kb on - strand, within AO356_25035 at 5673.150 kb on - strand, within AO356_25035 at 5673.156 kb on + strand, within AO356_25035 at 5673.156 kb on + strand, within AO356_25035 at 5673.156 kb on + strand, within AO356_25035 at 5673.442 kb on - strand, within AO356_25035 at 5673.442 kb on - strand, within AO356_25035 at 5673.442 kb on - strand, within AO356_25035 at 5673.442 kb on - strand, within AO356_25035 at 5673.594 kb on + strand, within AO356_25035 at 5673.594 kb on + strand, within AO356_25035 at 5673.595 kb on - strand, within AO356_25035 at 5673.595 kb on - strand, within AO356_25035 at 5673.734 kb on - strand at 5673.734 kb on - strand at 5673.851 kb on + strand at 5673.975 kb on + strand, within AO356_25040 at 5674.044 kb on + strand, within AO356_25040 at 5674.405 kb on + strand at 5674.406 kb on - strand at 5674.406 kb on - strand at 5674.420 kb on + strand at 5674.420 kb on + strand at 5674.420 kb on + strand at 5674.420 kb on + strand at 5674.420 kb on + strand at 5674.420 kb on + strand at 5674.421 kb on - strand at 5674.421 kb on - strand at 5674.552 kb on + strand, within AO356_25045 at 5674.553 kb on - strand, within AO356_25045 at 5674.689 kb on + strand, within AO356_25045 at 5674.689 kb on + strand, within AO356_25045
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,671,581 + +0.2 5,671,581 + +0.4 5,671,581 + +1.3 5,671,581 + +1.2 5,671,582 - -0.1 5,671,961 + AO356_25030 0.34 -0.1 5,671,962 - AO356_25030 0.34 +0.9 5,671,962 - AO356_25030 0.34 +0.1 5,671,962 - AO356_25030 0.34 +0.1 5,671,962 - AO356_25030 0.34 -0.3 5,672,093 + AO356_25030 0.50 -0.6 5,672,162 + AO356_25030 0.58 -1.1 5,672,162 + AO356_25030 0.58 +1.6 5,672,163 - AO356_25030 0.58 +1.0 5,672,309 + AO356_25030 0.76 +0.6 5,672,829 + AO356_25035 0.23 +0.6 5,672,829 + AO356_25035 0.23 +1.2 5,672,829 + AO356_25035 0.23 +2.0 5,672,830 - AO356_25035 0.23 +0.2 5,672,830 - AO356_25035 0.23 +0.7 5,672,928 + AO356_25035 0.32 -1.9 5,672,928 + AO356_25035 0.32 +0.2 5,673,120 + AO356_25035 0.48 -0.4 5,673,120 + AO356_25035 0.48 +0.5 5,673,121 - AO356_25035 0.48 +0.8 5,673,121 - AO356_25035 0.48 -0.7 5,673,138 + AO356_25035 0.49 -0.3 5,673,138 + AO356_25035 0.49 +0.9 5,673,139 - AO356_25035 0.50 +1.2 5,673,150 - AO356_25035 0.50 +0.1 5,673,156 + AO356_25035 0.51 +0.1 5,673,156 + AO356_25035 0.51 +0.9 5,673,156 + AO356_25035 0.51 +0.9 5,673,442 - AO356_25035 0.75 +0.1 5,673,442 - AO356_25035 0.75 +1.7 5,673,442 - AO356_25035 0.75 +1.9 5,673,442 - AO356_25035 0.75 -2.3 5,673,594 + AO356_25035 0.88 -1.6 5,673,594 + AO356_25035 0.88 -0.5 5,673,595 - AO356_25035 0.88 -3.0 5,673,595 - AO356_25035 0.88 +0.5 5,673,734 - +0.5 5,673,734 - +0.2 5,673,851 + +0.7 5,673,975 + AO356_25040 0.22 +1.3 5,674,044 + AO356_25040 0.34 +2.9 5,674,405 + +0.0 5,674,406 - +1.1 5,674,406 - -2.2 5,674,420 + -2.1 5,674,420 + +1.3 5,674,420 + +0.3 5,674,420 + -0.0 5,674,420 + +0.4 5,674,420 + +1.5 5,674,421 - +0.2 5,674,421 - -2.7 5,674,552 + AO356_25045 0.36 -1.4 5,674,553 - AO356_25045 0.36 +0.6 5,674,689 + AO356_25045 0.67 -2.6 5,674,689 + AO356_25045 0.67 +0.2
Or see this region's nucleotide sequence