Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22945 and AO356_22950 are separated by 145 nucleotides AO356_22950 and AO356_22955 are separated by 44 nucleotides AO356_22955 and AO356_22960 are separated by 66 nucleotides AO356_22960 and AO356_22965 are separated by 109 nucleotides
AO356_22945: AO356_22945 - peptidase M4, at 5,123,271 to 5,124,332
_22945
AO356_22950: AO356_22950 - peptidylprolyl isomerase, at 5,124,478 to 5,124,819
_22950
AO356_22955: AO356_22955 - ArsR family transcriptional regulator, at 5,124,864 to 5,125,631
_22955
AO356_22960: AO356_22960 - AraC family transcriptional regulator, at 5,125,698 to 5,126,489
_22960
AO356_22965: AO356_22965 - hypothetical protein, at 5,126,599 to 5,127,528
_22965
Position (kb) 5124
5125
5126 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5123.889 kb on + strand, within AO356_22945 at 5123.890 kb on - strand, within AO356_22945 at 5123.890 kb on - strand, within AO356_22945 at 5123.890 kb on - strand, within AO356_22945 at 5123.924 kb on - strand, within AO356_22945 at 5124.010 kb on + strand, within AO356_22945 at 5124.010 kb on + strand, within AO356_22945 at 5124.010 kb on + strand, within AO356_22945 at 5124.011 kb on - strand, within AO356_22945 at 5124.011 kb on - strand, within AO356_22945 at 5124.031 kb on + strand, within AO356_22945 at 5124.032 kb on - strand, within AO356_22945 at 5124.211 kb on - strand, within AO356_22945 at 5124.292 kb on + strand at 5124.292 kb on + strand at 5124.292 kb on + strand at 5124.292 kb on + strand at 5124.292 kb on + strand at 5124.344 kb on + strand at 5124.344 kb on + strand at 5124.381 kb on + strand at 5124.473 kb on - strand at 5124.530 kb on + strand, within AO356_22950 at 5124.601 kb on + strand, within AO356_22950 at 5124.886 kb on + strand at 5124.887 kb on - strand at 5124.887 kb on - strand at 5124.887 kb on - strand at 5124.887 kb on - strand at 5125.083 kb on + strand, within AO356_22955 at 5125.084 kb on - strand, within AO356_22955 at 5125.086 kb on + strand, within AO356_22955 at 5125.086 kb on + strand, within AO356_22955 at 5125.087 kb on - strand, within AO356_22955 at 5125.706 kb on + strand at 5125.706 kb on + strand at 5125.707 kb on - strand at 5125.707 kb on - strand at 5125.707 kb on - strand at 5125.995 kb on + strand, within AO356_22960 at 5126.032 kb on + strand, within AO356_22960 at 5126.032 kb on + strand, within AO356_22960 at 5126.033 kb on - strand, within AO356_22960 at 5126.218 kb on + strand, within AO356_22960 at 5126.219 kb on - strand, within AO356_22960 at 5126.219 kb on - strand, within AO356_22960 at 5126.308 kb on + strand, within AO356_22960 at 5126.309 kb on - strand, within AO356_22960 at 5126.309 kb on - strand, within AO356_22960 at 5126.309 kb on - strand, within AO356_22960 at 5126.347 kb on + strand, within AO356_22960 at 5126.348 kb on - strand, within AO356_22960 at 5126.348 kb on - strand, within AO356_22960 at 5126.389 kb on + strand, within AO356_22960 at 5126.536 kb on + strand at 5126.537 kb on - strand at 5126.537 kb on - strand at 5126.537 kb on - strand at 5126.539 kb on - strand at 5126.539 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,123,889 + AO356_22945 0.58 -0.2 5,123,890 - AO356_22945 0.58 +1.1 5,123,890 - AO356_22945 0.58 -1.0 5,123,890 - AO356_22945 0.58 -1.7 5,123,924 - AO356_22945 0.61 +0.5 5,124,010 + AO356_22945 0.70 +0.4 5,124,010 + AO356_22945 0.70 -0.2 5,124,010 + AO356_22945 0.70 -0.2 5,124,011 - AO356_22945 0.70 -2.3 5,124,011 - AO356_22945 0.70 -0.9 5,124,031 + AO356_22945 0.72 -1.6 5,124,032 - AO356_22945 0.72 +0.1 5,124,211 - AO356_22945 0.89 +1.3 5,124,292 + +0.2 5,124,292 + +1.3 5,124,292 + -0.5 5,124,292 + +0.1 5,124,292 + -1.2 5,124,344 + +0.3 5,124,344 + +1.5 5,124,381 + +2.7 5,124,473 - -3.0 5,124,530 + AO356_22950 0.15 -1.3 5,124,601 + AO356_22950 0.36 +1.2 5,124,886 + +0.6 5,124,887 - +0.8 5,124,887 - -1.1 5,124,887 - +0.4 5,124,887 - -1.6 5,125,083 + AO356_22955 0.29 +0.7 5,125,084 - AO356_22955 0.29 +1.4 5,125,086 + AO356_22955 0.29 -1.2 5,125,086 + AO356_22955 0.29 -0.3 5,125,087 - AO356_22955 0.29 +0.2 5,125,706 + -0.7 5,125,706 + +0.7 5,125,707 - -0.3 5,125,707 - +0.0 5,125,707 - +1.1 5,125,995 + AO356_22960 0.38 +2.1 5,126,032 + AO356_22960 0.42 -2.6 5,126,032 + AO356_22960 0.42 -1.1 5,126,033 - AO356_22960 0.42 +1.4 5,126,218 + AO356_22960 0.66 -0.4 5,126,219 - AO356_22960 0.66 -0.3 5,126,219 - AO356_22960 0.66 +0.3 5,126,308 + AO356_22960 0.77 +0.7 5,126,309 - AO356_22960 0.77 -0.4 5,126,309 - AO356_22960 0.77 -0.3 5,126,309 - AO356_22960 0.77 -2.4 5,126,347 + AO356_22960 0.82 -1.0 5,126,348 - AO356_22960 0.82 +0.9 5,126,348 - AO356_22960 0.82 +0.7 5,126,389 + AO356_22960 0.87 -0.3 5,126,536 + -2.5 5,126,537 - +2.5 5,126,537 - +0.3 5,126,537 - -1.0 5,126,539 - +0.4 5,126,539 - +1.0
Or see this region's nucleotide sequence