Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22175 and AO356_22180 are separated by 58 nucleotides AO356_22180 and AO356_22185 are separated by 214 nucleotides AO356_22185 and AO356_22190 overlap by 4 nucleotides AO356_22190 and AO356_22195 are separated by 15 nucleotides
AO356_22175: AO356_22175 - cell division protein FtsK, at 4,963,107 to 4,965,512
_22175
AO356_22180: AO356_22180 - outer membrane lipoprotein carrier protein LolA, at 4,965,571 to 4,966,194
_22180
AO356_22185: AO356_22185 - recombination factor protein RarA, at 4,966,409 to 4,967,734
_22185
AO356_22190: AO356_22190 - camphor resistance protein CrcB, at 4,967,731 to 4,968,105
_22190
AO356_22195: AO356_22195 - serine--tRNA ligase, at 4,968,121 to 4,969,401
_22195
Position (kb)
4966
4967
4968 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4965.484 kb on + strand at 4965.484 kb on + strand at 4966.192 kb on + strand at 4966.192 kb on + strand at 4966.192 kb on + strand at 4966.193 kb on - strand at 4966.193 kb on - strand at 4966.193 kb on - strand at 4966.193 kb on - strand at 4966.193 kb on - strand at 4966.402 kb on - strand at 4966.405 kb on - strand at 4966.405 kb on - strand at 4966.405 kb on - strand at 4966.405 kb on - strand at 4966.482 kb on - strand at 4966.482 kb on - strand at 4966.712 kb on + strand, within AO356_22185 at 4966.787 kb on + strand, within AO356_22185 at 4966.788 kb on - strand, within AO356_22185 at 4966.880 kb on + strand, within AO356_22185 at 4966.880 kb on + strand, within AO356_22185 at 4966.881 kb on - strand, within AO356_22185 at 4966.881 kb on - strand, within AO356_22185 at 4966.881 kb on - strand, within AO356_22185 at 4966.881 kb on - strand, within AO356_22185 at 4967.154 kb on - strand, within AO356_22185 at 4967.213 kb on + strand, within AO356_22185 at 4967.214 kb on - strand, within AO356_22185 at 4967.255 kb on + strand, within AO356_22185 at 4967.255 kb on + strand, within AO356_22185 at 4967.256 kb on - strand, within AO356_22185 at 4967.256 kb on - strand, within AO356_22185 at 4967.256 kb on - strand, within AO356_22185 at 4967.256 kb on - strand, within AO356_22185 at 4967.402 kb on + strand, within AO356_22185 at 4967.435 kb on + strand, within AO356_22185 at 4967.435 kb on + strand, within AO356_22185 at 4967.435 kb on + strand, within AO356_22185 at 4967.435 kb on + strand, within AO356_22185 at 4967.436 kb on - strand, within AO356_22185 at 4967.436 kb on - strand, within AO356_22185 at 4967.436 kb on - strand, within AO356_22185 at 4967.552 kb on + strand, within AO356_22185 at 4967.553 kb on - strand, within AO356_22185 at 4967.600 kb on + strand, within AO356_22185 at 4967.600 kb on + strand, within AO356_22185 at 4967.600 kb on + strand, within AO356_22185 at 4967.633 kb on + strand at 4967.633 kb on + strand at 4967.744 kb on + strand at 4967.744 kb on + strand at 4967.744 kb on + strand at 4967.833 kb on + strand, within AO356_22190 at 4967.833 kb on + strand, within AO356_22190 at 4967.833 kb on + strand, within AO356_22190 at 4967.833 kb on + strand, within AO356_22190 at 4967.885 kb on + strand, within AO356_22190 at 4967.885 kb on + strand, within AO356_22190 at 4967.885 kb on + strand, within AO356_22190 at 4967.885 kb on + strand, within AO356_22190 at 4967.887 kb on + strand, within AO356_22190 at 4968.037 kb on + strand, within AO356_22190
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,965,484 + +1.7 4,965,484 + +1.5 4,966,192 + +1.2 4,966,192 + -0.0 4,966,192 + -1.4 4,966,193 - +0.0 4,966,193 - -0.2 4,966,193 - +0.5 4,966,193 - -2.3 4,966,193 - -0.6 4,966,402 - -0.5 4,966,405 - -0.1 4,966,405 - +0.5 4,966,405 - -0.1 4,966,405 - -0.1 4,966,482 - +0.3 4,966,482 - -1.7 4,966,712 + AO356_22185 0.23 -0.6 4,966,787 + AO356_22185 0.29 -1.1 4,966,788 - AO356_22185 0.29 +0.8 4,966,880 + AO356_22185 0.36 -1.3 4,966,880 + AO356_22185 0.36 -0.6 4,966,881 - AO356_22185 0.36 -0.2 4,966,881 - AO356_22185 0.36 +0.3 4,966,881 - AO356_22185 0.36 -0.4 4,966,881 - AO356_22185 0.36 -0.6 4,967,154 - AO356_22185 0.56 -0.7 4,967,213 + AO356_22185 0.61 -0.2 4,967,214 - AO356_22185 0.61 -2.8 4,967,255 + AO356_22185 0.64 +0.6 4,967,255 + AO356_22185 0.64 -0.2 4,967,256 - AO356_22185 0.64 +0.8 4,967,256 - AO356_22185 0.64 +1.7 4,967,256 - AO356_22185 0.64 -1.0 4,967,256 - AO356_22185 0.64 -0.4 4,967,402 + AO356_22185 0.75 -2.7 4,967,435 + AO356_22185 0.77 -1.3 4,967,435 + AO356_22185 0.77 +2.1 4,967,435 + AO356_22185 0.77 +2.0 4,967,435 + AO356_22185 0.77 +0.4 4,967,436 - AO356_22185 0.77 -1.5 4,967,436 - AO356_22185 0.77 +0.4 4,967,436 - AO356_22185 0.77 -0.4 4,967,552 + AO356_22185 0.86 +0.1 4,967,553 - AO356_22185 0.86 +1.7 4,967,600 + AO356_22185 0.90 +0.7 4,967,600 + AO356_22185 0.90 +0.6 4,967,600 + AO356_22185 0.90 +0.3 4,967,633 + -0.6 4,967,633 + +0.3 4,967,744 + -1.0 4,967,744 + +0.1 4,967,744 + +2.1 4,967,833 + AO356_22190 0.27 -0.0 4,967,833 + AO356_22190 0.27 -0.9 4,967,833 + AO356_22190 0.27 +0.8 4,967,833 + AO356_22190 0.27 -0.7 4,967,885 + AO356_22190 0.41 -1.7 4,967,885 + AO356_22190 0.41 -0.5 4,967,885 + AO356_22190 0.41 +0.4 4,967,885 + AO356_22190 0.41 -0.0 4,967,887 + AO356_22190 0.42 +0.1 4,968,037 + AO356_22190 0.82 -0.9
Or see this region's nucleotide sequence