Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_20095 and AO356_20100 are separated by 279 nucleotides AO356_20100 and AO356_20105 overlap by 4 nucleotides
AO356_20095: AO356_20095 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 4,495,464 to 4,496,483
_20095
AO356_20100: AO356_20100 - (2Fe-2S)-binding protein, at 4,496,763 to 4,497,233
_20100
AO356_20105: AO356_20105 - acylaldehyde oxidase, at 4,497,230 to 4,499,545
_20105
Position (kb)
4496
4497
4498 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4496.598 kb on - strand at 4496.606 kb on - strand at 4496.627 kb on + strand at 4496.628 kb on - strand at 4496.628 kb on - strand at 4496.662 kb on + strand at 4496.663 kb on - strand at 4496.663 kb on - strand at 4496.663 kb on - strand at 4496.663 kb on - strand at 4496.663 kb on - strand at 4496.745 kb on + strand at 4496.746 kb on - strand at 4496.746 kb on - strand at 4496.746 kb on - strand at 4496.746 kb on - strand at 4496.768 kb on + strand at 4496.846 kb on - strand, within AO356_20100 at 4496.846 kb on - strand, within AO356_20100 at 4497.057 kb on + strand, within AO356_20100 at 4497.057 kb on + strand, within AO356_20100 at 4497.171 kb on + strand, within AO356_20100 at 4497.171 kb on + strand, within AO356_20100 at 4497.171 kb on + strand, within AO356_20100 at 4497.171 kb on + strand, within AO356_20100 at 4497.171 kb on + strand, within AO356_20100 at 4497.172 kb on - strand, within AO356_20100 at 4497.172 kb on - strand, within AO356_20100 at 4497.172 kb on - strand, within AO356_20100 at 4497.413 kb on + strand at 4497.413 kb on + strand at 4497.414 kb on - strand at 4497.414 kb on - strand at 4497.617 kb on + strand, within AO356_20105 at 4497.617 kb on + strand, within AO356_20105 at 4497.759 kb on - strand, within AO356_20105 at 4497.843 kb on - strand, within AO356_20105 at 4497.938 kb on + strand, within AO356_20105 at 4497.965 kb on + strand, within AO356_20105 at 4497.965 kb on + strand, within AO356_20105 at 4498.039 kb on - strand, within AO356_20105 at 4498.083 kb on + strand, within AO356_20105 at 4498.089 kb on + strand, within AO356_20105 at 4498.169 kb on + strand, within AO356_20105 at 4498.170 kb on - strand, within AO356_20105
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,496,598 - -2.2 4,496,606 - +1.1 4,496,627 + +2.7 4,496,628 - +0.2 4,496,628 - +0.3 4,496,662 + -2.8 4,496,663 - -0.3 4,496,663 - +1.2 4,496,663 - +0.8 4,496,663 - -0.4 4,496,663 - -0.3 4,496,745 + -0.4 4,496,746 - +1.6 4,496,746 - -0.7 4,496,746 - -0.5 4,496,746 - +1.3 4,496,768 + -2.0 4,496,846 - AO356_20100 0.18 -1.5 4,496,846 - AO356_20100 0.18 +0.5 4,497,057 + AO356_20100 0.62 -0.6 4,497,057 + AO356_20100 0.62 +1.4 4,497,171 + AO356_20100 0.87 -0.6 4,497,171 + AO356_20100 0.87 +0.6 4,497,171 + AO356_20100 0.87 +0.8 4,497,171 + AO356_20100 0.87 +1.3 4,497,171 + AO356_20100 0.87 +0.5 4,497,172 - AO356_20100 0.87 +0.7 4,497,172 - AO356_20100 0.87 -0.4 4,497,172 - AO356_20100 0.87 -0.9 4,497,413 + +0.1 4,497,413 + -0.1 4,497,414 - -0.3 4,497,414 - -1.0 4,497,617 + AO356_20105 0.17 -1.7 4,497,617 + AO356_20105 0.17 +1.2 4,497,759 - AO356_20105 0.23 -1.0 4,497,843 - AO356_20105 0.26 -2.0 4,497,938 + AO356_20105 0.31 -0.2 4,497,965 + AO356_20105 0.32 +1.2 4,497,965 + AO356_20105 0.32 +0.6 4,498,039 - AO356_20105 0.35 +2.0 4,498,083 + AO356_20105 0.37 +0.2 4,498,089 + AO356_20105 0.37 -2.0 4,498,169 + AO356_20105 0.41 +1.1 4,498,170 - AO356_20105 0.41 -1.5
Or see this region's nucleotide sequence