Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_18335 and AO356_18340 are separated by 54 nucleotides AO356_18340 and AO356_18345 are separated by 107 nucleotides AO356_18345 and AO356_18350 overlap by 4 nucleotides AO356_18350 and AO356_18355 overlap by 8 nucleotides AO356_18355 and AO356_18360 overlap by 4 nucleotides
AO356_18335: AO356_18335 - LuxR family transcriptional regulator, at 4,130,657 to 4,131,283
_18335
AO356_18340: AO356_18340 - leucyl-tRNA synthetase, at 4,131,338 to 4,131,523
_18340
AO356_18345: AO356_18345 - endonuclease III, at 4,131,631 to 4,132,269
_18345
AO356_18350: AO356_18350 - NADH:quinone oxidoreductase, at 4,132,266 to 4,132,799
_18350
AO356_18355: AO356_18355 - electron transporter RnfG, at 4,132,792 to 4,133,400
_18355
AO356_18360: AO356_18360 - electron transporter RnfD, at 4,133,397 to 4,134,377
_18360
Position (kb)
4132
4133 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4131.268 kb on + strand at 4131.269 kb on - strand at 4131.299 kb on + strand at 4131.299 kb on + strand at 4131.300 kb on - strand at 4131.300 kb on - strand at 4131.306 kb on + strand at 4131.306 kb on + strand at 4131.307 kb on - strand at 4131.332 kb on + strand at 4131.332 kb on + strand at 4131.332 kb on + strand at 4131.332 kb on + strand at 4131.332 kb on + strand at 4131.332 kb on + strand at 4131.332 kb on + strand at 4131.333 kb on - strand at 4131.333 kb on - strand at 4131.354 kb on + strand at 4131.354 kb on + strand at 4131.354 kb on + strand at 4131.375 kb on - strand, within AO356_18340 at 4131.434 kb on + strand, within AO356_18340 at 4131.494 kb on + strand, within AO356_18340 at 4131.494 kb on + strand, within AO356_18340 at 4131.494 kb on + strand, within AO356_18340 at 4131.494 kb on + strand, within AO356_18340 at 4131.494 kb on + strand, within AO356_18340 at 4131.494 kb on + strand, within AO356_18340 at 4131.495 kb on - strand, within AO356_18340 at 4131.495 kb on - strand, within AO356_18340 at 4131.541 kb on - strand at 4131.541 kb on - strand at 4131.562 kb on + strand at 4131.562 kb on + strand at 4131.562 kb on + strand at 4131.562 kb on + strand at 4131.562 kb on + strand at 4131.562 kb on + strand at 4131.563 kb on - strand at 4131.563 kb on - strand at 4131.563 kb on - strand at 4131.563 kb on - strand at 4131.564 kb on + strand at 4131.564 kb on + strand at 4131.564 kb on + strand at 4131.565 kb on - strand at 4131.565 kb on - strand at 4131.608 kb on - strand at 4131.624 kb on + strand at 4131.656 kb on + strand at 4131.656 kb on + strand at 4131.656 kb on + strand at 4131.656 kb on + strand at 4131.657 kb on - strand at 4131.657 kb on - strand at 4131.657 kb on - strand at 4131.665 kb on - strand at 4131.717 kb on - strand, within AO356_18345 at 4131.717 kb on - strand, within AO356_18345 at 4131.717 kb on - strand, within AO356_18345 at 4131.758 kb on + strand, within AO356_18345 at 4131.759 kb on - strand, within AO356_18345 at 4131.759 kb on - strand, within AO356_18345 at 4131.759 kb on - strand, within AO356_18345 at 4131.759 kb on - strand, within AO356_18345 at 4131.829 kb on - strand, within AO356_18345 at 4132.047 kb on - strand, within AO356_18345 at 4132.047 kb on - strand, within AO356_18345 at 4132.076 kb on + strand, within AO356_18345 at 4132.076 kb on + strand, within AO356_18345 at 4132.077 kb on - strand, within AO356_18345 at 4132.077 kb on - strand, within AO356_18345 at 4132.113 kb on + strand, within AO356_18345 at 4132.193 kb on + strand, within AO356_18345 at 4132.193 kb on + strand, within AO356_18345 at 4132.194 kb on - strand, within AO356_18345 at 4132.194 kb on - strand, within AO356_18345 at 4132.243 kb on + strand at 4132.243 kb on + strand at 4132.244 kb on - strand at 4132.335 kb on + strand, within AO356_18350 at 4132.335 kb on + strand, within AO356_18350 at 4132.535 kb on - strand, within AO356_18350 at 4132.535 kb on - strand, within AO356_18350 at 4132.552 kb on + strand, within AO356_18350 at 4132.587 kb on + strand, within AO356_18350 at 4132.587 kb on + strand, within AO356_18350 at 4132.587 kb on + strand, within AO356_18350 at 4132.587 kb on + strand, within AO356_18350 at 4132.587 kb on + strand, within AO356_18350 at 4132.588 kb on - strand, within AO356_18350 at 4132.588 kb on - strand, within AO356_18350 at 4132.588 kb on - strand, within AO356_18350 at 4132.588 kb on - strand, within AO356_18350 at 4132.713 kb on + strand, within AO356_18350 at 4132.713 kb on + strand, within AO356_18350 at 4132.714 kb on - strand, within AO356_18350 at 4132.793 kb on + strand at 4132.793 kb on + strand at 4132.793 kb on + strand at 4132.838 kb on + strand at 4132.839 kb on - strand at 4132.839 kb on - strand at 4133.187 kb on + strand, within AO356_18355 at 4133.187 kb on + strand, within AO356_18355 at 4133.188 kb on - strand, within AO356_18355 at 4133.188 kb on - strand, within AO356_18355 at 4133.188 kb on - strand, within AO356_18355 at 4133.188 kb on - strand, within AO356_18355 at 4133.188 kb on - strand, within AO356_18355 at 4133.243 kb on + strand, within AO356_18355 at 4133.243 kb on + strand, within AO356_18355 at 4133.336 kb on + strand, within AO356_18355 at 4133.336 kb on + strand, within AO356_18355 at 4133.336 kb on + strand, within AO356_18355 at 4133.337 kb on - strand, within AO356_18355 at 4133.337 kb on - strand, within AO356_18355 at 4133.337 kb on - strand, within AO356_18355 at 4133.696 kb on + strand, within AO356_18360 at 4133.697 kb on - strand, within AO356_18360
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,131,268 + +0.8 4,131,269 - +0.6 4,131,299 + -1.3 4,131,299 + -0.6 4,131,300 - +0.9 4,131,300 - -0.1 4,131,306 + -1.6 4,131,306 + -1.4 4,131,307 - -0.3 4,131,332 + -0.8 4,131,332 + +0.9 4,131,332 + +0.3 4,131,332 + +1.0 4,131,332 + -1.6 4,131,332 + +1.4 4,131,332 + +0.2 4,131,333 - -1.3 4,131,333 - +2.3 4,131,354 + +1.0 4,131,354 + +1.8 4,131,354 + +1.7 4,131,375 - AO356_18340 0.20 -0.4 4,131,434 + AO356_18340 0.52 +2.6 4,131,494 + AO356_18340 0.84 -0.7 4,131,494 + AO356_18340 0.84 -1.5 4,131,494 + AO356_18340 0.84 +1.1 4,131,494 + AO356_18340 0.84 +2.2 4,131,494 + AO356_18340 0.84 +2.5 4,131,494 + AO356_18340 0.84 +1.0 4,131,495 - AO356_18340 0.84 -0.2 4,131,495 - AO356_18340 0.84 +1.0 4,131,541 - -0.6 4,131,541 - +0.8 4,131,562 + -0.3 4,131,562 + -1.0 4,131,562 + +0.2 4,131,562 + -1.5 4,131,562 + -1.9 4,131,562 + +0.3 4,131,563 - -0.8 4,131,563 - -0.3 4,131,563 - +0.4 4,131,563 - -2.2 4,131,564 + +0.5 4,131,564 + +1.5 4,131,564 + +0.2 4,131,565 - +1.0 4,131,565 - -1.2 4,131,608 - -0.6 4,131,624 + +0.2 4,131,656 + +1.8 4,131,656 + -1.0 4,131,656 + +0.5 4,131,656 + +0.1 4,131,657 - -1.3 4,131,657 - -0.0 4,131,657 - -2.8 4,131,665 - +0.9 4,131,717 - AO356_18345 0.13 -0.0 4,131,717 - AO356_18345 0.13 +2.8 4,131,717 - AO356_18345 0.13 -0.4 4,131,758 + AO356_18345 0.20 -0.6 4,131,759 - AO356_18345 0.20 -0.4 4,131,759 - AO356_18345 0.20 +0.7 4,131,759 - AO356_18345 0.20 +0.2 4,131,759 - AO356_18345 0.20 +1.1 4,131,829 - AO356_18345 0.31 -1.4 4,132,047 - AO356_18345 0.65 -0.3 4,132,047 - AO356_18345 0.65 -1.6 4,132,076 + AO356_18345 0.70 -1.7 4,132,076 + AO356_18345 0.70 -1.1 4,132,077 - AO356_18345 0.70 +1.0 4,132,077 - AO356_18345 0.70 -0.2 4,132,113 + AO356_18345 0.75 -0.4 4,132,193 + AO356_18345 0.88 -0.2 4,132,193 + AO356_18345 0.88 +2.1 4,132,194 - AO356_18345 0.88 -0.6 4,132,194 - AO356_18345 0.88 -1.1 4,132,243 + +1.5 4,132,243 + +1.2 4,132,244 - +1.1 4,132,335 + AO356_18350 0.13 -3.9 4,132,335 + AO356_18350 0.13 +1.9 4,132,535 - AO356_18350 0.50 -1.3 4,132,535 - AO356_18350 0.50 +1.5 4,132,552 + AO356_18350 0.54 -0.1 4,132,587 + AO356_18350 0.60 -3.2 4,132,587 + AO356_18350 0.60 +2.8 4,132,587 + AO356_18350 0.60 -0.9 4,132,587 + AO356_18350 0.60 -1.2 4,132,587 + AO356_18350 0.60 +0.8 4,132,588 - AO356_18350 0.60 -1.1 4,132,588 - AO356_18350 0.60 -0.1 4,132,588 - AO356_18350 0.60 -0.3 4,132,588 - AO356_18350 0.60 -1.5 4,132,713 + AO356_18350 0.84 -0.1 4,132,713 + AO356_18350 0.84 +1.1 4,132,714 - AO356_18350 0.84 -0.3 4,132,793 + -0.7 4,132,793 + -1.8 4,132,793 + -2.4 4,132,838 + +0.3 4,132,839 - +0.7 4,132,839 - +0.8 4,133,187 + AO356_18355 0.65 +1.4 4,133,187 + AO356_18355 0.65 +1.0 4,133,188 - AO356_18355 0.65 -0.1 4,133,188 - AO356_18355 0.65 -1.0 4,133,188 - AO356_18355 0.65 -1.0 4,133,188 - AO356_18355 0.65 -0.2 4,133,188 - AO356_18355 0.65 +1.6 4,133,243 + AO356_18355 0.74 -1.0 4,133,243 + AO356_18355 0.74 +1.7 4,133,336 + AO356_18355 0.89 -0.9 4,133,336 + AO356_18355 0.89 +1.4 4,133,336 + AO356_18355 0.89 -1.5 4,133,337 - AO356_18355 0.89 -1.4 4,133,337 - AO356_18355 0.89 +0.4 4,133,337 - AO356_18355 0.89 -2.4 4,133,696 + AO356_18360 0.30 +0.8 4,133,697 - AO356_18360 0.31 -2.3
Or see this region's nucleotide sequence