Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_16455

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_16450 and AO356_16455 are separated by 22 nucleotidesAO356_16455 and AO356_16460 are separated by 166 nucleotidesAO356_16460 and AO356_16465 are separated by 142 nucleotides AO356_16450: AO356_16450 - GTP-binding protein, at 3,719,041 to 3,720,141 _16450 AO356_16455: AO356_16455 - peptidyl-tRNA hydrolase, at 3,720,164 to 3,720,748 _16455 AO356_16460: AO356_16460 - 50S ribosomal protein L25, at 3,720,915 to 3,721,517 _16460 AO356_16465: AO356_16465 - ribose-phosphate pyrophosphokinase, at 3,721,660 to 3,722,601 _16465 Position (kb) 3720 3721Strain fitness (log2 ratio) -2 -1 0 1at 3719.288 kb on + strand, within AO356_16450at 3719.289 kb on - strand, within AO356_16450at 3719.289 kb on - strand, within AO356_16450at 3719.525 kb on + strand, within AO356_16450at 3719.525 kb on + strand, within AO356_16450at 3719.525 kb on + strand, within AO356_16450at 3719.525 kb on + strand, within AO356_16450at 3719.525 kb on + strand, within AO356_16450at 3719.525 kb on + strand, within AO356_16450at 3719.525 kb on + strand, within AO356_16450at 3719.526 kb on - strand, within AO356_16450at 3719.562 kb on + strand, within AO356_16450at 3720.108 kb on + strandat 3720.158 kb on + strandat 3720.158 kb on + strandat 3720.158 kb on + strandat 3720.159 kb on - strandat 3720.737 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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3,719,288 + AO356_16450 0.22 -1.3
3,719,289 - AO356_16450 0.23 -0.8
3,719,289 - AO356_16450 0.23 -0.0
3,719,525 + AO356_16450 0.44 +1.5
3,719,525 + AO356_16450 0.44 -0.5
3,719,525 + AO356_16450 0.44 -1.6
3,719,525 + AO356_16450 0.44 -2.2
3,719,525 + AO356_16450 0.44 -0.4
3,719,525 + AO356_16450 0.44 -1.2
3,719,525 + AO356_16450 0.44 -0.3
3,719,526 - AO356_16450 0.44 +0.2
3,719,562 + AO356_16450 0.47 -1.8
3,720,108 + -1.7
3,720,158 + -0.1
3,720,158 + +0.1
3,720,158 + -0.8
3,720,159 - +0.9
3,720,737 - +1.0

Or see this region's nucleotide sequence