Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_04580 and AO356_04585 overlap by 32 nucleotides AO356_04585 and AO356_04590 overlap by 17 nucleotides AO356_04590 and AO356_04595 are separated by 149 nucleotides
AO356_04580: AO356_04580 - L-aspartate oxidase, at 1,032,222 to 1,033,838
_04580
AO356_04585: AO356_04585 - hypothetical protein, at 1,033,807 to 1,034,259
_04585
AO356_04590: AO356_04590 - hypothetical protein, at 1,034,243 to 1,034,497
_04590
AO356_04595: AO356_04595 - aminomethyltransferase, at 1,034,647 to 1,035,588
_04595
Position (kb)
1034
1035 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1033.275 kb on + strand, within AO356_04580 at 1033.275 kb on + strand, within AO356_04580 at 1033.275 kb on + strand, within AO356_04580 at 1033.276 kb on - strand, within AO356_04580 at 1033.276 kb on - strand, within AO356_04580 at 1033.277 kb on + strand, within AO356_04580 at 1033.277 kb on + strand, within AO356_04580 at 1033.277 kb on + strand, within AO356_04580 at 1033.277 kb on + strand, within AO356_04580 at 1033.277 kb on + strand, within AO356_04580 at 1033.277 kb on + strand, within AO356_04580 at 1033.277 kb on + strand, within AO356_04580 at 1033.277 kb on + strand, within AO356_04580 at 1033.278 kb on - strand, within AO356_04580 at 1033.278 kb on - strand, within AO356_04580 at 1033.278 kb on - strand, within AO356_04580 at 1033.278 kb on - strand, within AO356_04580 at 1033.332 kb on + strand, within AO356_04580 at 1033.332 kb on + strand, within AO356_04580 at 1033.332 kb on + strand, within AO356_04580 at 1033.333 kb on - strand, within AO356_04580 at 1033.333 kb on - strand, within AO356_04580 at 1033.333 kb on - strand, within AO356_04580 at 1033.333 kb on - strand, within AO356_04580 at 1033.333 kb on - strand, within AO356_04580 at 1033.569 kb on + strand, within AO356_04580 at 1033.569 kb on + strand, within AO356_04580 at 1033.569 kb on + strand, within AO356_04580 at 1033.570 kb on - strand, within AO356_04580 at 1033.570 kb on - strand, within AO356_04580 at 1033.653 kb on + strand, within AO356_04580 at 1033.654 kb on - strand, within AO356_04580 at 1033.662 kb on + strand, within AO356_04580 at 1033.662 kb on + strand, within AO356_04580 at 1033.662 kb on + strand, within AO356_04580 at 1033.662 kb on + strand, within AO356_04580 at 1033.663 kb on - strand, within AO356_04580 at 1033.663 kb on - strand, within AO356_04580 at 1033.663 kb on - strand, within AO356_04580 at 1033.663 kb on - strand, within AO356_04580 at 1033.764 kb on + strand at 1033.764 kb on + strand at 1033.764 kb on + strand at 1033.764 kb on + strand at 1033.764 kb on + strand at 1033.765 kb on - strand at 1033.765 kb on - strand at 1033.765 kb on - strand at 1033.808 kb on + strand at 1033.808 kb on + strand at 1033.809 kb on - strand at 1033.809 kb on - strand at 1033.809 kb on - strand at 1033.827 kb on + strand at 1033.827 kb on + strand at 1033.827 kb on + strand at 1033.828 kb on - strand at 1033.828 kb on - strand at 1033.968 kb on + strand, within AO356_04585 at 1034.244 kb on + strand at 1034.244 kb on + strand at 1034.244 kb on + strand at 1034.244 kb on + strand at 1034.245 kb on - strand at 1034.245 kb on - strand at 1034.245 kb on - strand at 1034.245 kb on - strand at 1034.245 kb on - strand at 1034.514 kb on - strand at 1034.514 kb on - strand at 1034.525 kb on - strand at 1034.525 kb on - strand at 1034.526 kb on + strand at 1034.526 kb on + strand at 1034.526 kb on + strand at 1034.527 kb on - strand at 1034.527 kb on - strand at 1034.527 kb on - strand at 1034.527 kb on - strand at 1034.587 kb on + strand at 1034.610 kb on + strand at 1034.611 kb on - strand at 1034.611 kb on - strand at 1034.611 kb on - strand at 1034.619 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,033,275 + AO356_04580 0.65 -0.6 1,033,275 + AO356_04580 0.65 -0.2 1,033,275 + AO356_04580 0.65 +1.3 1,033,276 - AO356_04580 0.65 -1.7 1,033,276 - AO356_04580 0.65 -3.5 1,033,277 + AO356_04580 0.65 +0.3 1,033,277 + AO356_04580 0.65 -0.2 1,033,277 + AO356_04580 0.65 -0.0 1,033,277 + AO356_04580 0.65 +0.6 1,033,277 + AO356_04580 0.65 -1.3 1,033,277 + AO356_04580 0.65 -1.1 1,033,277 + AO356_04580 0.65 -2.6 1,033,277 + AO356_04580 0.65 -1.8 1,033,278 - AO356_04580 0.65 -2.0 1,033,278 - AO356_04580 0.65 -0.9 1,033,278 - AO356_04580 0.65 -0.6 1,033,278 - AO356_04580 0.65 -1.6 1,033,332 + AO356_04580 0.69 +0.1 1,033,332 + AO356_04580 0.69 +2.3 1,033,332 + AO356_04580 0.69 -1.4 1,033,333 - AO356_04580 0.69 -1.9 1,033,333 - AO356_04580 0.69 -2.5 1,033,333 - AO356_04580 0.69 -1.0 1,033,333 - AO356_04580 0.69 -0.6 1,033,333 - AO356_04580 0.69 -0.7 1,033,569 + AO356_04580 0.83 -0.0 1,033,569 + AO356_04580 0.83 +0.2 1,033,569 + AO356_04580 0.83 -1.9 1,033,570 - AO356_04580 0.83 -1.1 1,033,570 - AO356_04580 0.83 -2.3 1,033,653 + AO356_04580 0.88 -2.3 1,033,654 - AO356_04580 0.89 -2.3 1,033,662 + AO356_04580 0.89 -1.9 1,033,662 + AO356_04580 0.89 +2.3 1,033,662 + AO356_04580 0.89 +0.0 1,033,662 + AO356_04580 0.89 +1.1 1,033,663 - AO356_04580 0.89 -2.1 1,033,663 - AO356_04580 0.89 +0.4 1,033,663 - AO356_04580 0.89 -1.8 1,033,663 - AO356_04580 0.89 -1.9 1,033,764 + +1.4 1,033,764 + -0.9 1,033,764 + +1.1 1,033,764 + -0.1 1,033,764 + +0.6 1,033,765 - +0.1 1,033,765 - +1.4 1,033,765 - -0.7 1,033,808 + +0.8 1,033,808 + -1.9 1,033,809 - +1.6 1,033,809 - -2.6 1,033,809 - -2.1 1,033,827 + +0.7 1,033,827 + +1.1 1,033,827 + -0.4 1,033,828 - -0.2 1,033,828 - +3.9 1,033,968 + AO356_04585 0.36 +1.7 1,034,244 + -0.6 1,034,244 + -0.5 1,034,244 + -0.6 1,034,244 + -2.4 1,034,245 - -0.5 1,034,245 - -0.1 1,034,245 - -0.7 1,034,245 - -0.2 1,034,245 - -1.9 1,034,514 - +0.5 1,034,514 - -0.9 1,034,525 - -0.4 1,034,525 - -0.1 1,034,526 + -0.8 1,034,526 + -1.9 1,034,526 + -0.2 1,034,527 - +0.2 1,034,527 - +0.1 1,034,527 - -1.9 1,034,527 - +1.3 1,034,587 + +0.3 1,034,610 + -1.5 1,034,611 - -1.6 1,034,611 - -2.0 1,034,611 - -3.3 1,034,619 + +0.2
Or see this region's nucleotide sequence