Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_00305 and AO356_00310 are separated by 211 nucleotides AO356_00310 and AO356_00315 overlap by 8 nucleotides AO356_00315 and AO356_00320 are separated by 111 nucleotides
AO356_00305: AO356_00305 - hypothetical protein, at 56,659 to 57,279
_00305
AO356_00310: AO356_00310 - hypothetical protein, at 57,491 to 58,111
_00310
AO356_00315: AO356_00315 - nucleotidyltransferase, at 58,104 to 59,387
_00315
AO356_00320: AO356_00320 - hypothetical protein, at 59,499 to 60,803
_00320
Position (kb) 58
59
60 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 57.142 kb on - strand, within AO356_00305 at 57.185 kb on + strand, within AO356_00305 at 57.185 kb on + strand, within AO356_00305 at 57.185 kb on + strand, within AO356_00305 at 57.185 kb on + strand, within AO356_00305 at 57.185 kb on + strand, within AO356_00305 at 57.186 kb on - strand, within AO356_00305 at 57.200 kb on + strand, within AO356_00305 at 57.200 kb on + strand, within AO356_00305 at 57.200 kb on + strand, within AO356_00305 at 57.200 kb on + strand, within AO356_00305 at 57.201 kb on - strand, within AO356_00305 at 57.203 kb on + strand, within AO356_00305 at 57.204 kb on - strand, within AO356_00305 at 57.234 kb on + strand at 57.234 kb on + strand at 57.234 kb on + strand at 57.234 kb on + strand at 57.235 kb on - strand at 57.235 kb on - strand at 57.241 kb on + strand at 57.242 kb on - strand at 57.267 kb on + strand at 57.317 kb on + strand at 57.317 kb on + strand at 57.317 kb on + strand at 57.317 kb on + strand at 57.317 kb on + strand at 57.318 kb on - strand at 57.318 kb on - strand at 57.318 kb on - strand at 57.318 kb on - strand at 57.320 kb on - strand at 57.320 kb on - strand at 57.329 kb on + strand at 57.329 kb on + strand at 57.329 kb on + strand at 57.329 kb on + strand at 57.338 kb on + strand at 57.338 kb on + strand at 57.338 kb on + strand at 57.339 kb on - strand at 57.339 kb on - strand at 57.339 kb on - strand at 57.339 kb on - strand at 57.347 kb on + strand at 57.348 kb on - strand at 57.394 kb on - strand at 57.394 kb on - strand at 57.434 kb on + strand at 57.434 kb on + strand at 57.434 kb on + strand at 57.434 kb on + strand at 57.434 kb on + strand at 57.434 kb on + strand at 57.434 kb on + strand at 57.435 kb on - strand at 57.436 kb on + strand at 57.436 kb on + strand at 57.436 kb on + strand at 57.436 kb on + strand at 57.436 kb on + strand at 57.437 kb on - strand at 57.437 kb on - strand at 57.437 kb on - strand at 57.437 kb on - strand at 57.437 kb on - strand at 57.437 kb on - strand at 57.437 kb on - strand at 57.437 kb on - strand at 57.438 kb on + strand at 57.438 kb on + strand at 57.438 kb on + strand at 57.438 kb on + strand at 57.438 kb on + strand at 57.439 kb on - strand at 57.439 kb on - strand at 57.439 kb on - strand at 57.439 kb on - strand at 57.439 kb on - strand at 57.439 kb on - strand at 57.439 kb on - strand at 57.439 kb on - strand at 57.439 kb on - strand at 57.439 kb on - strand at 57.439 kb on - strand at 57.439 kb on - strand at 57.439 kb on - strand at 57.454 kb on - strand at 57.483 kb on + strand at 57.484 kb on - strand at 57.484 kb on - strand at 57.484 kb on - strand at 57.488 kb on + strand at 57.488 kb on + strand at 57.488 kb on + strand at 57.488 kb on + strand at 57.488 kb on + strand at 57.489 kb on - strand at 57.489 kb on - strand at 57.489 kb on - strand at 57.490 kb on + strand at 57.490 kb on + strand at 57.533 kb on + strand at 57.534 kb on - strand at 57.546 kb on - strand at 57.572 kb on + strand, within AO356_00310 at 57.707 kb on + strand, within AO356_00310 at 57.708 kb on - strand, within AO356_00310 at 57.708 kb on - strand, within AO356_00310 at 57.716 kb on + strand, within AO356_00310 at 57.716 kb on + strand, within AO356_00310 at 57.716 kb on + strand, within AO356_00310 at 57.716 kb on + strand, within AO356_00310 at 57.717 kb on - strand, within AO356_00310 at 57.717 kb on - strand, within AO356_00310 at 57.717 kb on - strand, within AO356_00310 at 57.717 kb on - strand, within AO356_00310 at 57.719 kb on + strand, within AO356_00310 at 57.720 kb on - strand, within AO356_00310 at 57.720 kb on - strand, within AO356_00310 at 57.740 kb on + strand, within AO356_00310 at 57.740 kb on + strand, within AO356_00310 at 57.740 kb on + strand, within AO356_00310 at 57.741 kb on - strand, within AO356_00310 at 57.741 kb on - strand, within AO356_00310 at 57.846 kb on + strand, within AO356_00310 at 57.846 kb on + strand, within AO356_00310 at 58.015 kb on - strand, within AO356_00310 at 58.109 kb on + strand at 58.109 kb on + strand at 58.109 kb on + strand at 58.110 kb on - strand at 58.209 kb on + strand at 58.212 kb on + strand at 58.212 kb on + strand at 58.213 kb on - strand at 58.213 kb on - strand at 58.218 kb on - strand at 58.249 kb on + strand, within AO356_00315 at 58.250 kb on - strand, within AO356_00315 at 58.250 kb on - strand, within AO356_00315 at 58.250 kb on - strand, within AO356_00315 at 58.250 kb on - strand, within AO356_00315 at 58.257 kb on + strand, within AO356_00315 at 58.257 kb on + strand, within AO356_00315 at 58.326 kb on + strand, within AO356_00315 at 58.327 kb on - strand, within AO356_00315 at 58.381 kb on + strand, within AO356_00315 at 58.381 kb on + strand, within AO356_00315 at 58.381 kb on + strand, within AO356_00315 at 58.381 kb on + strand, within AO356_00315 at 58.382 kb on - strand, within AO356_00315 at 58.438 kb on + strand, within AO356_00315 at 58.438 kb on + strand, within AO356_00315 at 58.438 kb on + strand, within AO356_00315 at 58.438 kb on + strand, within AO356_00315 at 58.438 kb on + strand, within AO356_00315 at 58.461 kb on + strand, within AO356_00315 at 58.462 kb on - strand, within AO356_00315 at 58.462 kb on - strand, within AO356_00315 at 58.477 kb on + strand, within AO356_00315 at 58.506 kb on + strand, within AO356_00315 at 58.506 kb on + strand, within AO356_00315 at 58.506 kb on + strand, within AO356_00315 at 58.506 kb on + strand, within AO356_00315 at 58.506 kb on + strand, within AO356_00315 at 58.507 kb on - strand, within AO356_00315 at 58.507 kb on - strand, within AO356_00315 at 58.507 kb on - strand, within AO356_00315 at 58.507 kb on - strand, within AO356_00315 at 58.507 kb on - strand, within AO356_00315 at 58.507 kb on - strand, within AO356_00315 at 58.507 kb on - strand, within AO356_00315 at 58.507 kb on - strand, within AO356_00315 at 58.512 kb on + strand, within AO356_00315 at 58.693 kb on - strand, within AO356_00315 at 58.719 kb on + strand, within AO356_00315 at 58.773 kb on + strand, within AO356_00315 at 58.774 kb on - strand, within AO356_00315 at 58.847 kb on + strand, within AO356_00315 at 58.847 kb on + strand, within AO356_00315 at 58.886 kb on - strand, within AO356_00315 at 58.886 kb on - strand, within AO356_00315 at 58.931 kb on + strand, within AO356_00315 at 58.959 kb on + strand, within AO356_00315 at 58.959 kb on + strand, within AO356_00315 at 58.959 kb on + strand, within AO356_00315 at 58.960 kb on - strand, within AO356_00315 at 59.123 kb on + strand, within AO356_00315 at 59.123 kb on + strand, within AO356_00315 at 59.123 kb on + strand, within AO356_00315 at 59.123 kb on + strand, within AO356_00315 at 59.123 kb on + strand, within AO356_00315 at 59.123 kb on + strand, within AO356_00315 at 59.128 kb on - strand, within AO356_00315 at 59.192 kb on + strand, within AO356_00315 at 59.319 kb on + strand at 59.319 kb on + strand at 59.319 kb on + strand at 59.320 kb on - strand at 59.320 kb on - strand at 59.320 kb on - strand at 59.320 kb on - strand at 59.389 kb on + strand at 59.389 kb on + strand at 59.390 kb on - strand at 59.390 kb on - strand at 59.390 kb on - strand at 59.413 kb on - strand at 59.413 kb on - strand at 59.447 kb on - strand at 59.508 kb on + strand at 59.508 kb on + strand at 59.508 kb on + strand at 59.508 kb on + strand at 59.533 kb on + strand at 59.533 kb on + strand at 59.593 kb on - strand at 59.617 kb on + strand at 59.700 kb on + strand, within AO356_00320 at 59.700 kb on + strand, within AO356_00320 at 59.700 kb on + strand, within AO356_00320 at 59.700 kb on + strand, within AO356_00320 at 59.700 kb on + strand, within AO356_00320 at 59.700 kb on + strand, within AO356_00320 at 59.773 kb on - strand, within AO356_00320 at 59.801 kb on - strand, within AO356_00320 at 59.827 kb on - strand, within AO356_00320 at 59.904 kb on + strand, within AO356_00320 at 59.904 kb on + strand, within AO356_00320 at 59.934 kb on + strand, within AO356_00320 at 59.934 kb on + strand, within AO356_00320 at 59.934 kb on + strand, within AO356_00320 at 59.934 kb on + strand, within AO356_00320 at 59.934 kb on + strand, within AO356_00320 at 59.934 kb on + strand, within AO356_00320 at 59.935 kb on - strand, within AO356_00320 at 59.935 kb on - strand, within AO356_00320 at 59.935 kb on - strand, within AO356_00320 at 59.935 kb on - strand, within AO356_00320 at 59.935 kb on - strand, within AO356_00320 at 59.935 kb on - strand, within AO356_00320 at 59.945 kb on + strand, within AO356_00320 at 59.945 kb on + strand, within AO356_00320 at 59.946 kb on - strand, within AO356_00320 at 59.966 kb on - strand, within AO356_00320 at 59.966 kb on - strand, within AO356_00320 at 59.966 kb on - strand, within AO356_00320 at 59.966 kb on - strand, within AO356_00320 at 59.984 kb on + strand, within AO356_00320 at 60.117 kb on + strand, within AO356_00320 at 60.118 kb on - strand, within AO356_00320 at 60.184 kb on + strand, within AO356_00320 at 60.184 kb on + strand, within AO356_00320 at 60.185 kb on - strand, within AO356_00320 at 60.203 kb on - strand, within AO356_00320 at 60.279 kb on + strand, within AO356_00320 at 60.279 kb on + strand, within AO356_00320 at 60.280 kb on - strand, within AO356_00320 at 60.325 kb on - strand, within AO356_00320 at 60.325 kb on - strand, within AO356_00320
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 57,142 - AO356_00305 0.78 +0.6 57,185 + AO356_00305 0.85 -0.0 57,185 + AO356_00305 0.85 +0.5 57,185 + AO356_00305 0.85 -1.2 57,185 + AO356_00305 0.85 -1.2 57,185 + AO356_00305 0.85 -1.6 57,186 - AO356_00305 0.85 +0.4 57,200 + AO356_00305 0.87 -1.0 57,200 + AO356_00305 0.87 +0.6 57,200 + AO356_00305 0.87 -0.1 57,200 + AO356_00305 0.87 -0.7 57,201 - AO356_00305 0.87 +1.6 57,203 + AO356_00305 0.88 -0.2 57,204 - AO356_00305 0.88 -0.5 57,234 + +1.1 57,234 + -1.2 57,234 + -0.5 57,234 + -1.6 57,235 - +0.4 57,235 - +0.4 57,241 + +0.3 57,242 - -2.2 57,267 + +1.7 57,317 + +0.8 57,317 + -0.7 57,317 + +0.7 57,317 + -1.7 57,317 + +0.5 57,318 - +1.7 57,318 - -0.7 57,318 - -1.5 57,318 - +0.1 57,320 - +1.7 57,320 - -0.2 57,329 + -0.0 57,329 + +1.7 57,329 + +0.8 57,329 + +0.8 57,338 + +0.3 57,338 + -0.1 57,338 + +0.6 57,339 - +0.9 57,339 - -0.1 57,339 - -0.8 57,339 - -0.1 57,347 + +0.3 57,348 - +0.8 57,394 - +0.5 57,394 - +1.6 57,434 + -0.5 57,434 + +0.8 57,434 + -1.2 57,434 + +0.9 57,434 + +0.5 57,434 + -1.6 57,434 + -2.8 57,435 - -2.6 57,436 + -0.3 57,436 + -0.2 57,436 + +0.3 57,436 + +0.6 57,436 + -0.5 57,437 - -0.0 57,437 - +0.8 57,437 - +0.0 57,437 - -0.6 57,437 - -0.9 57,437 - -1.0 57,437 - +1.1 57,437 - -0.3 57,438 + +0.7 57,438 + +0.1 57,438 + +1.9 57,438 + -0.5 57,438 + -1.4 57,439 - +0.1 57,439 - +1.3 57,439 - +0.2 57,439 - -0.5 57,439 - -0.3 57,439 - +2.4 57,439 - +1.9 57,439 - -1.0 57,439 - +0.2 57,439 - -2.2 57,439 - +0.9 57,439 - +1.8 57,439 - -0.8 57,454 - +2.0 57,483 + -0.2 57,484 - +0.7 57,484 - -0.1 57,484 - +1.6 57,488 + +0.2 57,488 + -0.1 57,488 + +0.6 57,488 + +0.1 57,488 + -0.6 57,489 - +0.7 57,489 - +0.7 57,489 - +2.5 57,490 + +0.8 57,490 + -1.8 57,533 + -0.0 57,534 - +0.5 57,546 - -2.6 57,572 + AO356_00310 0.13 +0.6 57,707 + AO356_00310 0.35 -0.2 57,708 - AO356_00310 0.35 +1.6 57,708 - AO356_00310 0.35 +0.3 57,716 + AO356_00310 0.36 +0.8 57,716 + AO356_00310 0.36 -1.2 57,716 + AO356_00310 0.36 +0.7 57,716 + AO356_00310 0.36 -1.5 57,717 - AO356_00310 0.36 +0.9 57,717 - AO356_00310 0.36 +1.1 57,717 - AO356_00310 0.36 -0.4 57,717 - AO356_00310 0.36 +1.3 57,719 + AO356_00310 0.37 -1.3 57,720 - AO356_00310 0.37 -2.8 57,720 - AO356_00310 0.37 +0.8 57,740 + AO356_00310 0.40 +1.4 57,740 + AO356_00310 0.40 +0.8 57,740 + AO356_00310 0.40 -0.3 57,741 - AO356_00310 0.40 +0.5 57,741 - AO356_00310 0.40 -0.3 57,846 + AO356_00310 0.57 +1.0 57,846 + AO356_00310 0.57 -0.2 58,015 - AO356_00310 0.84 +1.0 58,109 + +0.1 58,109 + -0.1 58,109 + -2.0 58,110 - +1.3 58,209 + -2.0 58,212 + +1.2 58,212 + -0.3 58,213 - +1.9 58,213 - -0.1 58,218 - -2.3 58,249 + AO356_00315 0.11 +0.5 58,250 - AO356_00315 0.11 -0.5 58,250 - AO356_00315 0.11 -1.4 58,250 - AO356_00315 0.11 -3.5 58,250 - AO356_00315 0.11 -1.5 58,257 + AO356_00315 0.12 -0.5 58,257 + AO356_00315 0.12 -0.3 58,326 + AO356_00315 0.17 -0.3 58,327 - AO356_00315 0.17 -1.8 58,381 + AO356_00315 0.22 -0.2 58,381 + AO356_00315 0.22 -0.4 58,381 + AO356_00315 0.22 +0.9 58,381 + AO356_00315 0.22 -0.9 58,382 - AO356_00315 0.22 -0.6 58,438 + AO356_00315 0.26 +1.2 58,438 + AO356_00315 0.26 +0.8 58,438 + AO356_00315 0.26 -0.7 58,438 + AO356_00315 0.26 -1.6 58,438 + AO356_00315 0.26 -0.1 58,461 + AO356_00315 0.28 -0.1 58,462 - AO356_00315 0.28 +2.5 58,462 - AO356_00315 0.28 +1.3 58,477 + AO356_00315 0.29 -1.9 58,506 + AO356_00315 0.31 -1.0 58,506 + AO356_00315 0.31 +0.4 58,506 + AO356_00315 0.31 +1.0 58,506 + AO356_00315 0.31 +1.6 58,506 + AO356_00315 0.31 -1.3 58,507 - AO356_00315 0.31 +1.9 58,507 - AO356_00315 0.31 +0.4 58,507 - AO356_00315 0.31 +1.0 58,507 - AO356_00315 0.31 -2.8 58,507 - AO356_00315 0.31 -0.1 58,507 - AO356_00315 0.31 -0.8 58,507 - AO356_00315 0.31 -0.6 58,507 - AO356_00315 0.31 +1.8 58,512 + AO356_00315 0.32 -0.4 58,693 - AO356_00315 0.46 -0.6 58,719 + AO356_00315 0.48 +0.3 58,773 + AO356_00315 0.52 +0.4 58,774 - AO356_00315 0.52 +0.7 58,847 + AO356_00315 0.58 +0.1 58,847 + AO356_00315 0.58 -0.7 58,886 - AO356_00315 0.61 +0.7 58,886 - AO356_00315 0.61 +0.1 58,931 + AO356_00315 0.64 -1.5 58,959 + AO356_00315 0.67 -0.6 58,959 + AO356_00315 0.67 +0.9 58,959 + AO356_00315 0.67 +0.2 58,960 - AO356_00315 0.67 -0.2 59,123 + AO356_00315 0.79 +1.1 59,123 + AO356_00315 0.79 +2.0 59,123 + AO356_00315 0.79 +0.8 59,123 + AO356_00315 0.79 -0.4 59,123 + AO356_00315 0.79 -0.5 59,123 + AO356_00315 0.79 +0.6 59,128 - AO356_00315 0.80 +1.1 59,192 + AO356_00315 0.85 +1.0 59,319 + +0.4 59,319 + +1.2 59,319 + -2.5 59,320 - +3.0 59,320 - -0.9 59,320 - +1.1 59,320 - +1.6 59,389 + +0.2 59,389 + +0.2 59,390 - -0.7 59,390 - +0.7 59,390 - +0.4 59,413 - -0.3 59,413 - +0.9 59,447 - +0.4 59,508 + -0.0 59,508 + +0.7 59,508 + +1.5 59,508 + +3.3 59,533 + -0.1 59,533 + +0.3 59,593 - +1.4 59,617 + +2.8 59,700 + AO356_00320 0.15 +0.5 59,700 + AO356_00320 0.15 +0.1 59,700 + AO356_00320 0.15 -0.3 59,700 + AO356_00320 0.15 -2.0 59,700 + AO356_00320 0.15 +0.7 59,700 + AO356_00320 0.15 +0.3 59,773 - AO356_00320 0.21 -2.0 59,801 - AO356_00320 0.23 +2.5 59,827 - AO356_00320 0.25 +1.3 59,904 + AO356_00320 0.31 -0.6 59,904 + AO356_00320 0.31 +0.3 59,934 + AO356_00320 0.33 +0.6 59,934 + AO356_00320 0.33 +1.1 59,934 + AO356_00320 0.33 +1.0 59,934 + AO356_00320 0.33 -2.0 59,934 + AO356_00320 0.33 +0.4 59,934 + AO356_00320 0.33 +1.2 59,935 - AO356_00320 0.33 +0.7 59,935 - AO356_00320 0.33 -0.3 59,935 - AO356_00320 0.33 +1.6 59,935 - AO356_00320 0.33 -0.7 59,935 - AO356_00320 0.33 +1.7 59,935 - AO356_00320 0.33 -1.1 59,945 + AO356_00320 0.34 -0.5 59,945 + AO356_00320 0.34 -1.0 59,946 - AO356_00320 0.34 +0.0 59,966 - AO356_00320 0.36 +0.6 59,966 - AO356_00320 0.36 -1.7 59,966 - AO356_00320 0.36 +0.8 59,966 - AO356_00320 0.36 -0.9 59,984 + AO356_00320 0.37 +0.5 60,117 + AO356_00320 0.47 +0.2 60,118 - AO356_00320 0.47 +0.5 60,184 + AO356_00320 0.52 +0.4 60,184 + AO356_00320 0.52 -2.7 60,185 - AO356_00320 0.53 +1.1 60,203 - AO356_00320 0.54 -0.1 60,279 + AO356_00320 0.60 -1.4 60,279 + AO356_00320 0.60 +0.4 60,280 - AO356_00320 0.60 +0.9 60,325 - AO356_00320 0.63 -0.5 60,325 - AO356_00320 0.63 +1.9
Or see this region's nucleotide sequence