Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_00130 and AO356_00135 are separated by 899 nucleotides AO356_00135 and AO356_00140 are separated by 70 nucleotides AO356_00140 and AO356_00150 are separated by 648 nucleotides
AO356_00130: AO356_00130 - XRE family transcriptional regulator, at 25,301 to 25,642
_00130
AO356_00135: AO356_00135 - hypothetical protein, at 26,542 to 27,123
_00135
AO356_00140: AO356_00140 - hypothetical protein, at 27,194 to 27,451
_00140
AO356_00150: AO356_00150 - ABC transporter substrate-binding protein, at 28,100 to 28,864
_00150
Position (kb) 26
27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 25.646 kb on + strand at 25.646 kb on + strand at 25.646 kb on + strand at 25.646 kb on + strand at 25.646 kb on + strand at 25.646 kb on + strand at 25.719 kb on + strand at 25.719 kb on + strand at 25.719 kb on + strand at 25.720 kb on - strand at 25.720 kb on - strand at 25.908 kb on + strand at 25.908 kb on + strand at 26.066 kb on + strand at 26.066 kb on + strand at 26.066 kb on + strand at 26.066 kb on + strand at 26.066 kb on + strand at 26.066 kb on + strand at 26.066 kb on + strand at 26.067 kb on - strand at 26.067 kb on - strand at 26.067 kb on - strand at 26.067 kb on - strand at 26.067 kb on - strand at 26.067 kb on - strand at 26.067 kb on - strand at 26.067 kb on - strand at 26.081 kb on - strand at 26.133 kb on - strand at 26.144 kb on + strand at 26.144 kb on + strand at 26.145 kb on - strand at 26.155 kb on + strand at 26.190 kb on - strand at 26.200 kb on + strand at 26.200 kb on + strand at 26.201 kb on - strand at 26.201 kb on - strand at 26.202 kb on + strand at 26.202 kb on + strand at 26.203 kb on - strand at 26.203 kb on - strand at 26.203 kb on - strand at 26.203 kb on - strand at 26.203 kb on - strand at 26.244 kb on + strand at 26.244 kb on + strand at 26.264 kb on - strand at 26.347 kb on - strand at 26.347 kb on - strand at 26.349 kb on + strand at 26.350 kb on - strand at 26.578 kb on + strand at 26.578 kb on + strand at 26.578 kb on + strand at 26.630 kb on + strand, within AO356_00135 at 26.631 kb on - strand, within AO356_00135 at 26.631 kb on - strand, within AO356_00135 at 26.704 kb on + strand, within AO356_00135 at 26.761 kb on + strand, within AO356_00135 at 26.762 kb on - strand, within AO356_00135 at 26.762 kb on - strand, within AO356_00135 at 26.763 kb on + strand, within AO356_00135 at 26.764 kb on - strand, within AO356_00135 at 26.764 kb on - strand, within AO356_00135 at 26.764 kb on - strand, within AO356_00135 at 26.840 kb on - strand, within AO356_00135 at 26.854 kb on - strand, within AO356_00135 at 26.896 kb on + strand, within AO356_00135 at 26.896 kb on + strand, within AO356_00135 at 26.896 kb on + strand, within AO356_00135 at 26.897 kb on - strand, within AO356_00135 at 27.105 kb on + strand at 27.105 kb on + strand at 27.106 kb on - strand at 27.106 kb on - strand at 27.106 kb on - strand at 27.106 kb on - strand at 27.106 kb on - strand at 27.106 kb on - strand at 27.139 kb on + strand at 27.139 kb on + strand at 27.139 kb on + strand at 27.140 kb on - strand at 27.140 kb on - strand at 27.140 kb on - strand at 27.239 kb on + strand, within AO356_00140 at 27.248 kb on + strand, within AO356_00140 at 27.248 kb on + strand, within AO356_00140 at 27.248 kb on + strand, within AO356_00140 at 27.249 kb on - strand, within AO356_00140 at 27.267 kb on - strand, within AO356_00140 at 27.372 kb on + strand, within AO356_00140 at 27.373 kb on - strand, within AO356_00140 at 27.373 kb on - strand, within AO356_00140 at 27.411 kb on + strand, within AO356_00140 at 27.411 kb on + strand, within AO356_00140 at 27.411 kb on + strand, within AO356_00140 at 27.411 kb on + strand, within AO356_00140 at 27.411 kb on + strand, within AO356_00140 at 27.412 kb on - strand, within AO356_00140 at 27.412 kb on - strand, within AO356_00140 at 27.412 kb on - strand, within AO356_00140 at 27.565 kb on + strand at 27.565 kb on + strand at 27.704 kb on + strand at 27.704 kb on + strand at 27.705 kb on - strand at 27.861 kb on + strand at 28.042 kb on + strand at 28.052 kb on + strand at 28.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 25,646 + +0.5 25,646 + +0.8 25,646 + -0.4 25,646 + -0.4 25,646 + +0.0 25,646 + -0.6 25,719 + -1.6 25,719 + -1.3 25,719 + -1.4 25,720 - -0.7 25,720 - +0.0 25,908 + +1.5 25,908 + +1.5 26,066 + -0.8 26,066 + +1.5 26,066 + -0.4 26,066 + -0.1 26,066 + +0.4 26,066 + -0.1 26,066 + +0.3 26,067 - +0.9 26,067 - +0.1 26,067 - +1.4 26,067 - -1.6 26,067 - -0.6 26,067 - +0.5 26,067 - -0.7 26,067 - -0.1 26,081 - +1.7 26,133 - +0.4 26,144 + +1.0 26,144 + -0.6 26,145 - +0.8 26,155 + +1.6 26,190 - +0.4 26,200 + +1.4 26,200 + +1.1 26,201 - +2.6 26,201 - -0.9 26,202 + +0.7 26,202 + +0.5 26,203 - +1.5 26,203 - -2.9 26,203 - +0.6 26,203 - +0.1 26,203 - -0.8 26,244 + +2.0 26,244 + -1.7 26,264 - -1.9 26,347 - +2.0 26,347 - -0.6 26,349 + +2.6 26,350 - +0.8 26,578 + +0.3 26,578 + +0.6 26,578 + +0.9 26,630 + AO356_00135 0.15 +0.4 26,631 - AO356_00135 0.15 -3.0 26,631 - AO356_00135 0.15 +0.0 26,704 + AO356_00135 0.28 +0.5 26,761 + AO356_00135 0.38 +1.0 26,762 - AO356_00135 0.38 +2.9 26,762 - AO356_00135 0.38 +0.3 26,763 + AO356_00135 0.38 +1.5 26,764 - AO356_00135 0.38 -1.3 26,764 - AO356_00135 0.38 -0.9 26,764 - AO356_00135 0.38 +1.4 26,840 - AO356_00135 0.51 -0.3 26,854 - AO356_00135 0.54 +0.6 26,896 + AO356_00135 0.61 +1.4 26,896 + AO356_00135 0.61 +0.1 26,896 + AO356_00135 0.61 +0.8 26,897 - AO356_00135 0.61 +1.0 27,105 + -0.5 27,105 + +0.3 27,106 - -0.7 27,106 - +0.5 27,106 - +0.7 27,106 - +0.3 27,106 - +0.6 27,106 - +0.8 27,139 + -1.7 27,139 + +0.7 27,139 + -0.6 27,140 - -0.5 27,140 - -1.3 27,140 - +1.9 27,239 + AO356_00140 0.17 +1.1 27,248 + AO356_00140 0.21 -1.1 27,248 + AO356_00140 0.21 +1.6 27,248 + AO356_00140 0.21 -0.2 27,249 - AO356_00140 0.21 -0.7 27,267 - AO356_00140 0.28 +1.0 27,372 + AO356_00140 0.69 +3.5 27,373 - AO356_00140 0.69 +0.1 27,373 - AO356_00140 0.69 -0.4 27,411 + AO356_00140 0.84 -0.9 27,411 + AO356_00140 0.84 +2.1 27,411 + AO356_00140 0.84 -2.8 27,411 + AO356_00140 0.84 -2.8 27,411 + AO356_00140 0.84 +0.2 27,412 - AO356_00140 0.84 +1.0 27,412 - AO356_00140 0.84 +0.3 27,412 - AO356_00140 0.84 -0.8 27,565 + +1.7 27,565 + +0.3 27,704 + +0.1 27,704 + -0.9 27,705 - -2.6 27,861 + -0.3 28,042 + +2.0 28,052 + +0.2 28,053 - +2.1
Or see this region's nucleotide sequence