Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_26320 and AO356_26325 are separated by 27 nucleotides AO356_26325 and AO356_26330 are separated by 1 nucleotides AO356_26330 and AO356_26335 are separated by 67 nucleotides
AO356_26320: AO356_26320 - protein CapI, at 5,961,964 to 5,962,935
_26320
AO356_26325: AO356_26325 - dolichol-phosphate mannosyltransferase, at 5,962,963 to 5,963,706
_26325
AO356_26330: AO356_26330 - Lipid A biosynthesis, N-terminal, at 5,963,708 to 5,964,001
_26330
AO356_26335: AO356_26335 - dolichyl-phosphate-mannose-protein mannosyltransferase, at 5,964,069 to 5,965,526
_26335
Position (kb) 5963
5964
5965 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5962.921 kb on + strand at 5962.978 kb on + strand at 5962.978 kb on + strand at 5962.978 kb on + strand at 5962.979 kb on - strand at 5962.979 kb on - strand at 5962.979 kb on - strand at 5962.979 kb on - strand at 5962.979 kb on - strand at 5962.979 kb on - strand at 5963.200 kb on + strand, within AO356_26325 at 5963.201 kb on - strand, within AO356_26325 at 5963.502 kb on + strand, within AO356_26325 at 5963.502 kb on + strand, within AO356_26325 at 5963.599 kb on + strand, within AO356_26325 at 5963.660 kb on + strand at 5963.661 kb on - strand at 5963.790 kb on - strand, within AO356_26330 at 5963.798 kb on + strand, within AO356_26330 at 5963.870 kb on + strand, within AO356_26330 at 5963.879 kb on + strand, within AO356_26330 at 5963.880 kb on - strand, within AO356_26330 at 5964.193 kb on - strand at 5964.315 kb on + strand, within AO356_26335 at 5964.316 kb on - strand, within AO356_26335 at 5964.384 kb on + strand, within AO356_26335 at 5964.393 kb on + strand, within AO356_26335 at 5964.393 kb on + strand, within AO356_26335 at 5964.393 kb on + strand, within AO356_26335 at 5964.393 kb on + strand, within AO356_26335 at 5964.393 kb on + strand, within AO356_26335 at 5964.394 kb on - strand, within AO356_26335 at 5964.394 kb on - strand, within AO356_26335 at 5964.394 kb on - strand, within AO356_26335 at 5964.456 kb on + strand, within AO356_26335 at 5964.457 kb on - strand, within AO356_26335 at 5964.457 kb on - strand, within AO356_26335 at 5964.501 kb on + strand, within AO356_26335 at 5964.502 kb on - strand, within AO356_26335 at 5964.504 kb on + strand, within AO356_26335 at 5964.505 kb on - strand, within AO356_26335 at 5964.505 kb on - strand, within AO356_26335 at 5964.588 kb on + strand, within AO356_26335 at 5964.816 kb on + strand, within AO356_26335 at 5964.843 kb on + strand, within AO356_26335 at 5964.843 kb on + strand, within AO356_26335 at 5964.844 kb on - strand, within AO356_26335 at 5964.928 kb on + strand, within AO356_26335 at 5964.929 kb on - strand, within AO356_26335 at 5964.946 kb on + strand, within AO356_26335 at 5964.947 kb on - strand, within AO356_26335 at 5964.993 kb on + strand, within AO356_26335 at 5964.993 kb on + strand, within AO356_26335 at 5964.993 kb on + strand, within AO356_26335 at 5964.994 kb on - strand, within AO356_26335
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,962,921 + -0.6 5,962,978 + -0.4 5,962,978 + -0.9 5,962,978 + -0.6 5,962,979 - +0.6 5,962,979 - -0.3 5,962,979 - -0.0 5,962,979 - -1.6 5,962,979 - +0.3 5,962,979 - -0.2 5,963,200 + AO356_26325 0.32 -2.1 5,963,201 - AO356_26325 0.32 +0.1 5,963,502 + AO356_26325 0.72 +1.0 5,963,502 + AO356_26325 0.72 +1.0 5,963,599 + AO356_26325 0.85 +0.1 5,963,660 + +0.1 5,963,661 - -0.4 5,963,790 - AO356_26330 0.28 -1.9 5,963,798 + AO356_26330 0.31 -0.3 5,963,870 + AO356_26330 0.55 +1.3 5,963,879 + AO356_26330 0.58 -2.4 5,963,880 - AO356_26330 0.59 +0.6 5,964,193 - -1.8 5,964,315 + AO356_26335 0.17 +0.7 5,964,316 - AO356_26335 0.17 +0.1 5,964,384 + AO356_26335 0.22 +0.4 5,964,393 + AO356_26335 0.22 +1.0 5,964,393 + AO356_26335 0.22 +2.4 5,964,393 + AO356_26335 0.22 -0.9 5,964,393 + AO356_26335 0.22 +0.2 5,964,393 + AO356_26335 0.22 +0.4 5,964,394 - AO356_26335 0.22 -0.0 5,964,394 - AO356_26335 0.22 +1.2 5,964,394 - AO356_26335 0.22 +0.5 5,964,456 + AO356_26335 0.27 +0.9 5,964,457 - AO356_26335 0.27 -0.1 5,964,457 - AO356_26335 0.27 -0.9 5,964,501 + AO356_26335 0.30 +1.3 5,964,502 - AO356_26335 0.30 +0.2 5,964,504 + AO356_26335 0.30 +1.4 5,964,505 - AO356_26335 0.30 +0.7 5,964,505 - AO356_26335 0.30 -0.7 5,964,588 + AO356_26335 0.36 -1.8 5,964,816 + AO356_26335 0.51 -2.7 5,964,843 + AO356_26335 0.53 -0.0 5,964,843 + AO356_26335 0.53 +1.8 5,964,844 - AO356_26335 0.53 +2.6 5,964,928 + AO356_26335 0.59 +0.1 5,964,929 - AO356_26335 0.59 -2.9 5,964,946 + AO356_26335 0.60 +1.5 5,964,947 - AO356_26335 0.60 -0.7 5,964,993 + AO356_26335 0.63 +0.2 5,964,993 + AO356_26335 0.63 +0.6 5,964,993 + AO356_26335 0.63 -1.6 5,964,994 - AO356_26335 0.63 -1.4
Or see this region's nucleotide sequence