Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25765 and AO356_25770 overlap by 4 nucleotides AO356_25770 and AO356_25775 are separated by 44 nucleotides AO356_25775 and AO356_25780 are separated by 205 nucleotides
AO356_25765: AO356_25765 - carnitine dehydratase, at 5,824,859 to 5,826,340
_25765
AO356_25770: AO356_25770 - 3-oxoadipate enol-lactonase, at 5,826,337 to 5,827,170
_25770
AO356_25775: AO356_25775 - peptide synthetase, at 5,827,215 to 5,827,805
_25775
AO356_25780: AO356_25780 - hypothetical protein, at 5,828,011 to 5,829,498
_25780
Position (kb) 5827
5828 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5826.355 kb on + strand at 5826.428 kb on - strand, within AO356_25770 at 5826.438 kb on + strand, within AO356_25770 at 5826.438 kb on + strand, within AO356_25770 at 5826.438 kb on + strand, within AO356_25770 at 5826.439 kb on - strand, within AO356_25770 at 5826.439 kb on - strand, within AO356_25770 at 5826.584 kb on + strand, within AO356_25770 at 5826.584 kb on + strand, within AO356_25770 at 5826.584 kb on + strand, within AO356_25770 at 5826.611 kb on + strand, within AO356_25770 at 5826.612 kb on - strand, within AO356_25770 at 5826.612 kb on - strand, within AO356_25770 at 5826.692 kb on + strand, within AO356_25770 at 5826.692 kb on + strand, within AO356_25770 at 5827.127 kb on + strand at 5827.128 kb on - strand at 5827.255 kb on + strand at 5827.255 kb on + strand at 5827.255 kb on + strand at 5827.256 kb on - strand at 5827.363 kb on + strand, within AO356_25775 at 5827.363 kb on + strand, within AO356_25775 at 5827.363 kb on + strand, within AO356_25775 at 5827.363 kb on + strand, within AO356_25775 at 5827.364 kb on - strand, within AO356_25775 at 5827.565 kb on - strand, within AO356_25775 at 5827.774 kb on + strand at 5827.774 kb on + strand at 5827.774 kb on + strand at 5827.774 kb on + strand at 5827.775 kb on - strand at 5827.788 kb on - strand at 5827.827 kb on - strand at 5828.068 kb on + strand at 5828.069 kb on - strand at 5828.069 kb on - strand at 5828.269 kb on + strand, within AO356_25780 at 5828.270 kb on - strand, within AO356_25780 at 5828.395 kb on + strand, within AO356_25780 at 5828.396 kb on - strand, within AO356_25780 at 5828.396 kb on - strand, within AO356_25780 at 5828.396 kb on - strand, within AO356_25780 at 5828.396 kb on - strand, within AO356_25780 at 5828.396 kb on - strand, within AO356_25780 at 5828.398 kb on + strand, within AO356_25780 at 5828.399 kb on - strand, within AO356_25780 at 5828.401 kb on + strand, within AO356_25780 at 5828.413 kb on + strand, within AO356_25780 at 5828.413 kb on + strand, within AO356_25780 at 5828.413 kb on + strand, within AO356_25780 at 5828.414 kb on - strand, within AO356_25780 at 5828.486 kb on - strand, within AO356_25780 at 5828.530 kb on + strand, within AO356_25780 at 5828.531 kb on - strand, within AO356_25780 at 5828.531 kb on - strand, within AO356_25780 at 5828.637 kb on + strand, within AO356_25780 at 5828.637 kb on + strand, within AO356_25780
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,826,355 + +1.1 5,826,428 - AO356_25770 0.11 +0.0 5,826,438 + AO356_25770 0.12 +2.3 5,826,438 + AO356_25770 0.12 +1.4 5,826,438 + AO356_25770 0.12 -2.1 5,826,439 - AO356_25770 0.12 +0.2 5,826,439 - AO356_25770 0.12 +0.4 5,826,584 + AO356_25770 0.30 +0.2 5,826,584 + AO356_25770 0.30 -1.1 5,826,584 + AO356_25770 0.30 -1.3 5,826,611 + AO356_25770 0.33 +1.3 5,826,612 - AO356_25770 0.33 +0.5 5,826,612 - AO356_25770 0.33 -0.1 5,826,692 + AO356_25770 0.43 +2.1 5,826,692 + AO356_25770 0.43 -0.9 5,827,127 + +0.5 5,827,128 - +0.1 5,827,255 + +0.3 5,827,255 + +2.0 5,827,255 + +1.9 5,827,256 - -0.4 5,827,363 + AO356_25775 0.25 +1.3 5,827,363 + AO356_25775 0.25 -0.9 5,827,363 + AO356_25775 0.25 -1.2 5,827,363 + AO356_25775 0.25 +1.4 5,827,364 - AO356_25775 0.25 -0.2 5,827,565 - AO356_25775 0.59 -0.5 5,827,774 + -2.3 5,827,774 + -1.0 5,827,774 + +1.7 5,827,774 + -0.2 5,827,775 - +0.7 5,827,788 - -0.3 5,827,827 - -0.6 5,828,068 + +1.1 5,828,069 - +1.7 5,828,069 - +0.8 5,828,269 + AO356_25780 0.17 +0.6 5,828,270 - AO356_25780 0.17 -0.8 5,828,395 + AO356_25780 0.26 -1.5 5,828,396 - AO356_25780 0.26 -1.4 5,828,396 - AO356_25780 0.26 -0.1 5,828,396 - AO356_25780 0.26 +1.0 5,828,396 - AO356_25780 0.26 -0.2 5,828,396 - AO356_25780 0.26 -1.5 5,828,398 + AO356_25780 0.26 -2.0 5,828,399 - AO356_25780 0.26 -0.5 5,828,401 + AO356_25780 0.26 -0.9 5,828,413 + AO356_25780 0.27 -2.3 5,828,413 + AO356_25780 0.27 +1.1 5,828,413 + AO356_25780 0.27 +1.0 5,828,414 - AO356_25780 0.27 +1.6 5,828,486 - AO356_25780 0.32 -0.5 5,828,530 + AO356_25780 0.35 -1.2 5,828,531 - AO356_25780 0.35 -0.8 5,828,531 - AO356_25780 0.35 -1.0 5,828,637 + AO356_25780 0.42 +0.7 5,828,637 + AO356_25780 0.42 -2.3
Or see this region's nucleotide sequence