Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_25305

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_25295 and AO356_25300 overlap by 4 nucleotidesAO356_25300 and AO356_25305 overlap by 4 nucleotidesAO356_25305 and AO356_25310 are separated by 21 nucleotidesAO356_25310 and AO356_25315 are separated by 5 nucleotides AO356_25295: AO356_25295 - hypothetical protein, at 5,726,212 to 5,726,778 _25295 AO356_25300: AO356_25300 - hypothetical protein, at 5,726,775 to 5,727,314 _25300 AO356_25305: AO356_25305 - metal ABC transporter ATPase, at 5,727,311 to 5,727,616 _25305 AO356_25310: AO356_25310 - hypothetical protein, at 5,727,638 to 5,728,126 _25310 AO356_25315: AO356_25315 - cyclopropane-fatty-acyl-phospholipid synthase, at 5,728,132 to 5,729,394 _25315 Position (kb) 5727 5728Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5726.369 kb on - strand, within AO356_25295at 5726.570 kb on + strand, within AO356_25295at 5726.571 kb on - strand, within AO356_25295at 5726.626 kb on + strand, within AO356_25295at 5726.627 kb on - strand, within AO356_25295at 5726.627 kb on - strand, within AO356_25295at 5726.806 kb on + strandat 5727.177 kb on + strand, within AO356_25300at 5727.177 kb on + strand, within AO356_25300at 5727.177 kb on + strand, within AO356_25300at 5727.178 kb on - strand, within AO356_25300at 5727.205 kb on - strand, within AO356_25300at 5727.205 kb on - strand, within AO356_25300at 5727.393 kb on - strand, within AO356_25305at 5727.393 kb on - strand, within AO356_25305at 5727.396 kb on + strand, within AO356_25305at 5727.396 kb on + strand, within AO356_25305at 5727.396 kb on + strand, within AO356_25305at 5727.397 kb on - strand, within AO356_25305at 5727.397 kb on - strand, within AO356_25305at 5727.397 kb on - strand, within AO356_25305at 5727.427 kb on + strand, within AO356_25305at 5727.427 kb on + strand, within AO356_25305at 5727.428 kb on - strand, within AO356_25305at 5727.428 kb on - strand, within AO356_25305at 5727.615 kb on - strandat 5727.677 kb on + strandat 5727.677 kb on + strandat 5727.678 kb on - strandat 5727.762 kb on + strand, within AO356_25310at 5727.763 kb on - strand, within AO356_25310at 5727.833 kb on - strand, within AO356_25310at 5728.133 kb on + strandat 5728.230 kb on - strandat 5728.230 kb on - strandat 5728.238 kb on + strandat 5728.239 kb on - strandat 5728.271 kb on + strand, within AO356_25315at 5728.340 kb on + strand, within AO356_25315at 5728.340 kb on + strand, within AO356_25315at 5728.341 kb on - strand, within AO356_25315at 5728.563 kb on - strand, within AO356_25315

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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5,726,369 - AO356_25295 0.28 -3.4
5,726,570 + AO356_25295 0.63 +0.2
5,726,571 - AO356_25295 0.63 +0.6
5,726,626 + AO356_25295 0.73 +1.7
5,726,627 - AO356_25295 0.73 -1.1
5,726,627 - AO356_25295 0.73 -0.5
5,726,806 + +1.1
5,727,177 + AO356_25300 0.74 +1.6
5,727,177 + AO356_25300 0.74 +1.9
5,727,177 + AO356_25300 0.74 -1.0
5,727,178 - AO356_25300 0.75 -0.7
5,727,205 - AO356_25300 0.80 +0.8
5,727,205 - AO356_25300 0.80 -0.7
5,727,393 - AO356_25305 0.27 -0.2
5,727,393 - AO356_25305 0.27 +0.1
5,727,396 + AO356_25305 0.28 -0.1
5,727,396 + AO356_25305 0.28 +1.6
5,727,396 + AO356_25305 0.28 +1.0
5,727,397 - AO356_25305 0.28 -0.6
5,727,397 - AO356_25305 0.28 -0.7
5,727,397 - AO356_25305 0.28 -0.9
5,727,427 + AO356_25305 0.38 +2.5
5,727,427 + AO356_25305 0.38 +1.7
5,727,428 - AO356_25305 0.38 +0.1
5,727,428 - AO356_25305 0.38 -0.0
5,727,615 - -1.3
5,727,677 + +0.8
5,727,677 + -0.7
5,727,678 - -0.7
5,727,762 + AO356_25310 0.25 -2.4
5,727,763 - AO356_25310 0.26 -1.6
5,727,833 - AO356_25310 0.40 +0.5
5,728,133 + +0.7
5,728,230 - -0.7
5,728,230 - +0.9
5,728,238 + +0.0
5,728,239 - +0.9
5,728,271 + AO356_25315 0.11 +1.0
5,728,340 + AO356_25315 0.16 +2.5
5,728,340 + AO356_25315 0.16 +1.1
5,728,341 - AO356_25315 0.17 -0.6
5,728,563 - AO356_25315 0.34 +0.1

Or see this region's nucleotide sequence