Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25105 and AO356_25110 are separated by 4 nucleotides AO356_25110 and AO356_25115 overlap by 4 nucleotides AO356_25115 and AO356_25120 are separated by 129 nucleotides AO356_25120 and AO356_25125 are separated by 93 nucleotides
AO356_25105: AO356_25105 - nitric oxide reductase activation protein NorD, at 5,683,648 to 5,685,486
_25105
AO356_25110: AO356_25110 - protein DnrP, at 5,685,491 to 5,685,691
_25110
AO356_25115: AO356_25115 - transcription factor, at 5,685,688 to 5,686,371
_25115
AO356_25120: AO356_25120 - hypothetical protein, at 5,686,501 to 5,686,830
_25120
AO356_25125: AO356_25125 - short-chain dehydrogenase, at 5,686,924 to 5,688,114
_25125
Position (kb) 5685
5686
5687 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5685.049 kb on + strand, within AO356_25105 at 5685.049 kb on + strand, within AO356_25105 at 5685.116 kb on - strand, within AO356_25105 at 5685.163 kb on + strand, within AO356_25105 at 5685.164 kb on - strand, within AO356_25105 at 5685.164 kb on - strand, within AO356_25105 at 5685.164 kb on - strand, within AO356_25105 at 5685.164 kb on - strand, within AO356_25105 at 5685.278 kb on - strand, within AO356_25105 at 5685.355 kb on - strand at 5685.383 kb on - strand at 5685.391 kb on + strand at 5685.391 kb on + strand at 5685.391 kb on + strand at 5685.391 kb on + strand at 5685.391 kb on + strand at 5685.391 kb on + strand at 5685.391 kb on + strand at 5685.391 kb on + strand at 5685.412 kb on + strand at 5685.666 kb on + strand, within AO356_25110 at 5685.667 kb on - strand, within AO356_25110 at 5686.025 kb on + strand, within AO356_25115 at 5686.025 kb on + strand, within AO356_25115 at 5686.026 kb on - strand, within AO356_25115 at 5686.026 kb on - strand, within AO356_25115 at 5686.026 kb on - strand, within AO356_25115 at 5686.026 kb on - strand, within AO356_25115 at 5686.026 kb on - strand, within AO356_25115 at 5686.085 kb on + strand, within AO356_25115 at 5686.085 kb on + strand, within AO356_25115 at 5686.085 kb on + strand, within AO356_25115 at 5686.211 kb on + strand, within AO356_25115 at 5686.211 kb on + strand, within AO356_25115 at 5686.212 kb on - strand, within AO356_25115 at 5686.386 kb on + strand at 5686.387 kb on - strand at 5686.387 kb on - strand at 5686.387 kb on - strand at 5686.387 kb on - strand at 5686.394 kb on - strand at 5686.645 kb on + strand, within AO356_25120 at 5686.659 kb on - strand, within AO356_25120 at 5686.723 kb on + strand, within AO356_25120 at 5686.923 kb on + strand at 5686.923 kb on + strand at 5686.923 kb on + strand at 5686.924 kb on - strand at 5686.924 kb on - strand at 5686.924 kb on - strand at 5686.924 kb on - strand at 5686.924 kb on - strand at 5686.924 kb on - strand at 5686.924 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,685,049 + AO356_25105 0.76 +1.3 5,685,049 + AO356_25105 0.76 -1.1 5,685,116 - AO356_25105 0.80 +0.2 5,685,163 + AO356_25105 0.82 -2.1 5,685,164 - AO356_25105 0.82 -1.8 5,685,164 - AO356_25105 0.82 +1.5 5,685,164 - AO356_25105 0.82 -2.9 5,685,164 - AO356_25105 0.82 +1.3 5,685,278 - AO356_25105 0.89 -2.8 5,685,355 - -0.9 5,685,383 - +1.5 5,685,391 + -2.4 5,685,391 + +1.2 5,685,391 + -0.1 5,685,391 + -1.6 5,685,391 + +1.1 5,685,391 + +0.6 5,685,391 + +1.0 5,685,391 + +0.4 5,685,412 + +1.5 5,685,666 + AO356_25110 0.87 -0.0 5,685,667 - AO356_25110 0.88 +2.7 5,686,025 + AO356_25115 0.49 -0.3 5,686,025 + AO356_25115 0.49 +1.3 5,686,026 - AO356_25115 0.49 +1.8 5,686,026 - AO356_25115 0.49 -2.3 5,686,026 - AO356_25115 0.49 +0.1 5,686,026 - AO356_25115 0.49 -1.4 5,686,026 - AO356_25115 0.49 -0.2 5,686,085 + AO356_25115 0.58 -0.1 5,686,085 + AO356_25115 0.58 -0.4 5,686,085 + AO356_25115 0.58 +0.5 5,686,211 + AO356_25115 0.76 -2.6 5,686,211 + AO356_25115 0.76 +1.1 5,686,212 - AO356_25115 0.77 +3.3 5,686,386 + -2.0 5,686,387 - -1.8 5,686,387 - -1.2 5,686,387 - -0.2 5,686,387 - -0.4 5,686,394 - +0.6 5,686,645 + AO356_25120 0.44 -1.2 5,686,659 - AO356_25120 0.48 -0.1 5,686,723 + AO356_25120 0.67 +0.2 5,686,923 + +0.6 5,686,923 + +1.1 5,686,923 + +0.4 5,686,924 - +0.6 5,686,924 - +1.7 5,686,924 - -0.8 5,686,924 - +1.0 5,686,924 - +0.3 5,686,924 - -0.1 5,686,924 - +0.3
Or see this region's nucleotide sequence