Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23350 and AO356_23355 are separated by 43 nucleotides AO356_23355 and AO356_23360 are separated by 28 nucleotides AO356_23360 and AO356_23365 are separated by 1 nucleotides AO356_23365 and AO356_23370 are separated by 123 nucleotides
AO356_23350: AO356_23350 - S-transferase, at 5,237,172 to 5,238,011
_23350
AO356_23355: AO356_23355 - ABC transporter substrate-binding protein, at 5,238,055 to 5,238,789
_23355
AO356_23360: AO356_23360 - diguanylate cyclase, at 5,238,818 to 5,240,161
_23360
AO356_23365: AO356_23365 - amino acid ABC transporter substrate-binding protein, at 5,240,163 to 5,240,921
_23365
AO356_23370: AO356_23370 - aldo/keto reductase, at 5,241,045 to 5,242,040
_23370
Position (kb) 5238
5239
5240
5241 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5237.869 kb on + strand, within AO356_23350 at 5237.870 kb on - strand, within AO356_23350 at 5237.995 kb on + strand at 5237.995 kb on + strand at 5237.995 kb on + strand at 5238.005 kb on + strand at 5238.005 kb on + strand at 5238.006 kb on - strand at 5238.006 kb on - strand at 5238.019 kb on + strand at 5238.019 kb on + strand at 5238.019 kb on + strand at 5238.019 kb on + strand at 5238.019 kb on + strand at 5238.019 kb on + strand at 5238.019 kb on + strand at 5238.020 kb on - strand at 5238.020 kb on - strand at 5238.020 kb on - strand at 5238.020 kb on - strand at 5238.057 kb on - strand at 5238.197 kb on + strand, within AO356_23355 at 5238.197 kb on + strand, within AO356_23355 at 5238.197 kb on + strand, within AO356_23355 at 5238.197 kb on + strand, within AO356_23355 at 5238.198 kb on - strand, within AO356_23355 at 5238.198 kb on - strand, within AO356_23355 at 5238.198 kb on - strand, within AO356_23355 at 5238.198 kb on - strand, within AO356_23355 at 5238.198 kb on - strand, within AO356_23355 at 5238.198 kb on - strand, within AO356_23355 at 5238.362 kb on + strand, within AO356_23355 at 5238.401 kb on + strand, within AO356_23355 at 5238.402 kb on - strand, within AO356_23355 at 5238.481 kb on + strand, within AO356_23355 at 5238.481 kb on + strand, within AO356_23355 at 5238.482 kb on - strand, within AO356_23355 at 5238.482 kb on - strand, within AO356_23355 at 5238.572 kb on + strand, within AO356_23355 at 5238.690 kb on - strand, within AO356_23355 at 5238.690 kb on - strand, within AO356_23355 at 5238.754 kb on + strand at 5238.754 kb on + strand at 5238.755 kb on - strand at 5238.789 kb on + strand at 5238.789 kb on + strand at 5238.789 kb on + strand at 5238.790 kb on - strand at 5238.790 kb on - strand at 5238.790 kb on - strand at 5238.790 kb on - strand at 5238.861 kb on - strand at 5238.861 kb on - strand at 5238.861 kb on - strand at 5239.042 kb on + strand, within AO356_23360 at 5239.167 kb on + strand, within AO356_23360 at 5239.168 kb on - strand, within AO356_23360 at 5239.168 kb on - strand, within AO356_23360 at 5239.168 kb on - strand, within AO356_23360 at 5239.179 kb on + strand, within AO356_23360 at 5239.180 kb on - strand, within AO356_23360 at 5239.180 kb on - strand, within AO356_23360 at 5239.180 kb on - strand, within AO356_23360 at 5239.275 kb on + strand, within AO356_23360 at 5239.537 kb on + strand, within AO356_23360 at 5239.570 kb on + strand, within AO356_23360 at 5239.699 kb on + strand, within AO356_23360 at 5239.699 kb on + strand, within AO356_23360 at 5239.700 kb on - strand, within AO356_23360 at 5239.700 kb on - strand, within AO356_23360 at 5239.702 kb on + strand, within AO356_23360 at 5239.944 kb on + strand, within AO356_23360 at 5240.166 kb on + strand at 5240.167 kb on - strand at 5240.191 kb on + strand at 5240.192 kb on - strand at 5240.362 kb on + strand, within AO356_23365 at 5240.362 kb on + strand, within AO356_23365 at 5240.362 kb on + strand, within AO356_23365 at 5240.363 kb on - strand, within AO356_23365 at 5240.422 kb on + strand, within AO356_23365 at 5240.422 kb on + strand, within AO356_23365 at 5240.434 kb on + strand, within AO356_23365 at 5240.434 kb on + strand, within AO356_23365 at 5240.434 kb on + strand, within AO356_23365 at 5240.434 kb on + strand, within AO356_23365 at 5240.435 kb on - strand, within AO356_23365 at 5240.435 kb on - strand, within AO356_23365 at 5240.435 kb on - strand, within AO356_23365 at 5240.489 kb on - strand, within AO356_23365 at 5240.581 kb on + strand, within AO356_23365 at 5240.581 kb on + strand, within AO356_23365 at 5240.581 kb on + strand, within AO356_23365 at 5240.581 kb on + strand, within AO356_23365 at 5240.582 kb on - strand, within AO356_23365 at 5240.582 kb on - strand, within AO356_23365 at 5240.582 kb on - strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.588 kb on - strand, within AO356_23365 at 5240.588 kb on - strand, within AO356_23365 at 5240.588 kb on - strand, within AO356_23365 at 5240.588 kb on - strand, within AO356_23365 at 5240.645 kb on + strand, within AO356_23365 at 5240.659 kb on + strand, within AO356_23365 at 5240.659 kb on + strand, within AO356_23365 at 5240.809 kb on + strand, within AO356_23365 at 5240.809 kb on + strand, within AO356_23365 at 5240.809 kb on + strand, within AO356_23365 at 5240.809 kb on + strand, within AO356_23365 at 5240.810 kb on - strand, within AO356_23365 at 5240.969 kb on - strand at 5240.971 kb on + strand at 5240.971 kb on + strand at 5240.971 kb on + strand at 5240.972 kb on - strand at 5240.972 kb on - strand at 5240.972 kb on - strand at 5240.972 kb on - strand at 5240.977 kb on + strand at 5240.978 kb on - strand at 5240.978 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,237,869 + AO356_23350 0.83 -0.2 5,237,870 - AO356_23350 0.83 -0.9 5,237,995 + +0.9 5,237,995 + +0.5 5,237,995 + -1.8 5,238,005 + -1.0 5,238,005 + +2.2 5,238,006 - +0.4 5,238,006 - +2.1 5,238,019 + +1.0 5,238,019 + +0.7 5,238,019 + -0.0 5,238,019 + +0.6 5,238,019 + -2.6 5,238,019 + +1.2 5,238,019 + -0.4 5,238,020 - -1.1 5,238,020 - +1.7 5,238,020 - +1.2 5,238,020 - -0.5 5,238,057 - +1.4 5,238,197 + AO356_23355 0.19 +0.6 5,238,197 + AO356_23355 0.19 +1.2 5,238,197 + AO356_23355 0.19 +1.3 5,238,197 + AO356_23355 0.19 +0.5 5,238,198 - AO356_23355 0.19 -1.7 5,238,198 - AO356_23355 0.19 +0.2 5,238,198 - AO356_23355 0.19 -1.8 5,238,198 - AO356_23355 0.19 -0.2 5,238,198 - AO356_23355 0.19 +1.2 5,238,198 - AO356_23355 0.19 +1.7 5,238,362 + AO356_23355 0.42 +1.7 5,238,401 + AO356_23355 0.47 -1.1 5,238,402 - AO356_23355 0.47 +0.5 5,238,481 + AO356_23355 0.58 +1.6 5,238,481 + AO356_23355 0.58 +1.3 5,238,482 - AO356_23355 0.58 -0.4 5,238,482 - AO356_23355 0.58 +0.5 5,238,572 + AO356_23355 0.70 +2.0 5,238,690 - AO356_23355 0.86 +0.7 5,238,690 - AO356_23355 0.86 +2.8 5,238,754 + +0.3 5,238,754 + +0.7 5,238,755 - +0.5 5,238,789 + +0.8 5,238,789 + -0.7 5,238,789 + -0.2 5,238,790 - +0.5 5,238,790 - +1.0 5,238,790 - -0.3 5,238,790 - +1.6 5,238,861 - +1.0 5,238,861 - +2.2 5,238,861 - +2.4 5,239,042 + AO356_23360 0.17 +0.9 5,239,167 + AO356_23360 0.26 +0.5 5,239,168 - AO356_23360 0.26 +0.9 5,239,168 - AO356_23360 0.26 +1.5 5,239,168 - AO356_23360 0.26 +0.3 5,239,179 + AO356_23360 0.27 +0.5 5,239,180 - AO356_23360 0.27 -0.1 5,239,180 - AO356_23360 0.27 -0.5 5,239,180 - AO356_23360 0.27 -0.2 5,239,275 + AO356_23360 0.34 -0.1 5,239,537 + AO356_23360 0.53 -2.8 5,239,570 + AO356_23360 0.56 -2.0 5,239,699 + AO356_23360 0.66 +0.6 5,239,699 + AO356_23360 0.66 +0.2 5,239,700 - AO356_23360 0.66 +1.1 5,239,700 - AO356_23360 0.66 -1.7 5,239,702 + AO356_23360 0.66 -2.2 5,239,944 + AO356_23360 0.84 +1.4 5,240,166 + +0.3 5,240,167 - +0.5 5,240,191 + -2.3 5,240,192 - -3.7 5,240,362 + AO356_23365 0.26 -0.7 5,240,362 + AO356_23365 0.26 -1.3 5,240,362 + AO356_23365 0.26 +0.6 5,240,363 - AO356_23365 0.26 +0.2 5,240,422 + AO356_23365 0.34 +0.5 5,240,422 + AO356_23365 0.34 -0.7 5,240,434 + AO356_23365 0.36 -1.4 5,240,434 + AO356_23365 0.36 +0.0 5,240,434 + AO356_23365 0.36 -2.5 5,240,434 + AO356_23365 0.36 +3.0 5,240,435 - AO356_23365 0.36 -1.5 5,240,435 - AO356_23365 0.36 +0.0 5,240,435 - AO356_23365 0.36 -0.5 5,240,489 - AO356_23365 0.43 +0.8 5,240,581 + AO356_23365 0.55 +1.3 5,240,581 + AO356_23365 0.55 -1.4 5,240,581 + AO356_23365 0.55 +1.1 5,240,581 + AO356_23365 0.55 +0.3 5,240,582 - AO356_23365 0.55 -1.2 5,240,582 - AO356_23365 0.55 -0.7 5,240,582 - AO356_23365 0.55 +1.3 5,240,587 + AO356_23365 0.56 +1.0 5,240,587 + AO356_23365 0.56 -2.8 5,240,587 + AO356_23365 0.56 -1.0 5,240,587 + AO356_23365 0.56 +0.8 5,240,587 + AO356_23365 0.56 +2.6 5,240,587 + AO356_23365 0.56 +0.6 5,240,587 + AO356_23365 0.56 +1.8 5,240,588 - AO356_23365 0.56 +0.6 5,240,588 - AO356_23365 0.56 -1.1 5,240,588 - AO356_23365 0.56 +0.8 5,240,588 - AO356_23365 0.56 +0.0 5,240,645 + AO356_23365 0.64 +0.9 5,240,659 + AO356_23365 0.65 -0.5 5,240,659 + AO356_23365 0.65 +3.0 5,240,809 + AO356_23365 0.85 -2.0 5,240,809 + AO356_23365 0.85 -0.3 5,240,809 + AO356_23365 0.85 +0.5 5,240,809 + AO356_23365 0.85 +0.9 5,240,810 - AO356_23365 0.85 +0.4 5,240,969 - +0.9 5,240,971 + -0.5 5,240,971 + +1.6 5,240,971 + +0.6 5,240,972 - -0.8 5,240,972 - +2.2 5,240,972 - +0.3 5,240,972 - -0.6 5,240,977 + -0.1 5,240,978 - +0.2 5,240,978 - -2.0
Or see this region's nucleotide sequence