Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22255 and AO356_22260 are separated by 40 nucleotides AO356_22260 and AO356_22265 are separated by 60 nucleotides AO356_22265 and AO356_22270 are separated by 213 nucleotides
AO356_22255: AO356_22255 - gamma-glutamyltranspeptidase, at 4,976,702 to 4,978,429
_22255
AO356_22260: AO356_22260 - isochorismatase, at 4,978,470 to 4,979,003
_22260
AO356_22265: AO356_22265 - hypothetical protein, at 4,979,064 to 4,979,924
_22265
AO356_22270: AO356_22270 - hypothetical protein, at 4,980,138 to 4,981,103
_22270
Position (kb)
4979
4980 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4978.157 kb on + strand, within AO356_22255 at 4978.379 kb on + strand at 4978.379 kb on + strand at 4978.490 kb on + strand at 4978.731 kb on + strand, within AO356_22260 at 4978.731 kb on + strand, within AO356_22260 at 4978.806 kb on + strand, within AO356_22260 at 4978.806 kb on + strand, within AO356_22260 at 4978.975 kb on + strand at 4978.976 kb on - strand at 4979.038 kb on + strand at 4979.038 kb on + strand at 4979.038 kb on + strand at 4979.039 kb on - strand at 4979.039 kb on - strand at 4979.039 kb on - strand at 4979.039 kb on - strand at 4979.092 kb on + strand at 4979.092 kb on + strand at 4979.320 kb on + strand, within AO356_22265 at 4979.320 kb on + strand, within AO356_22265 at 4979.320 kb on + strand, within AO356_22265 at 4979.321 kb on - strand, within AO356_22265 at 4979.321 kb on - strand, within AO356_22265 at 4979.321 kb on - strand, within AO356_22265 at 4979.321 kb on - strand, within AO356_22265 at 4979.566 kb on + strand, within AO356_22265 at 4980.121 kb on + strand at 4980.122 kb on - strand at 4980.187 kb on + strand at 4980.188 kb on - strand at 4980.373 kb on + strand, within AO356_22270 at 4980.374 kb on - strand, within AO356_22270 at 4980.374 kb on - strand, within AO356_22270 at 4980.374 kb on - strand, within AO356_22270 at 4980.481 kb on - strand, within AO356_22270 at 4980.654 kb on + strand, within AO356_22270 at 4980.811 kb on + strand, within AO356_22270 at 4980.921 kb on + strand, within AO356_22270
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,978,157 + AO356_22255 0.84 -1.0 4,978,379 + +0.6 4,978,379 + -2.0 4,978,490 + -1.6 4,978,731 + AO356_22260 0.49 +0.5 4,978,731 + AO356_22260 0.49 -2.4 4,978,806 + AO356_22260 0.63 +0.4 4,978,806 + AO356_22260 0.63 -1.6 4,978,975 + +0.7 4,978,976 - -1.8 4,979,038 + -1.2 4,979,038 + -2.2 4,979,038 + -1.8 4,979,039 - -2.9 4,979,039 - +1.1 4,979,039 - +1.4 4,979,039 - +0.9 4,979,092 + -3.6 4,979,092 + +1.5 4,979,320 + AO356_22265 0.30 +1.4 4,979,320 + AO356_22265 0.30 -0.2 4,979,320 + AO356_22265 0.30 +1.4 4,979,321 - AO356_22265 0.30 +0.4 4,979,321 - AO356_22265 0.30 -0.5 4,979,321 - AO356_22265 0.30 +1.4 4,979,321 - AO356_22265 0.30 -0.6 4,979,566 + AO356_22265 0.58 +0.9 4,980,121 + -0.2 4,980,122 - +1.3 4,980,187 + +1.0 4,980,188 - +1.0 4,980,373 + AO356_22270 0.24 -0.5 4,980,374 - AO356_22270 0.24 +1.0 4,980,374 - AO356_22270 0.24 -1.8 4,980,374 - AO356_22270 0.24 +0.4 4,980,481 - AO356_22270 0.36 +0.4 4,980,654 + AO356_22270 0.53 +1.0 4,980,811 + AO356_22270 0.70 -0.3 4,980,921 + AO356_22270 0.81 -2.6
Or see this region's nucleotide sequence