Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_21830 and AO356_21835 are separated by 347 nucleotides AO356_21835 and AO356_21840 are separated by 175 nucleotides AO356_21840 and AO356_21845 are separated by 224 nucleotides AO356_21845 and AO356_21850 overlap by 1 nucleotides
AO356_21830: AO356_21830 - hypothetical protein, at 4,888,091 to 4,888,372
_21830
AO356_21835: AO356_21835 - glyoxalase, at 4,888,720 to 4,889,103
_21835
AO356_21840: AO356_21840 - N-methylproline demethylase, at 4,889,279 to 4,891,315
_21840
AO356_21845: AO356_21845 - electron transporter RnfB, at 4,891,540 to 4,892,289
_21845
AO356_21850: AO356_21850 - electron transfer flavoprotein subunit beta, at 4,892,289 to 4,893,224
_21850
Position (kb)
4889
4890
4891
4892 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4888.370 kb on + strand at 4888.370 kb on + strand at 4888.616 kb on + strand at 4888.616 kb on + strand at 4888.722 kb on - strand at 4888.722 kb on - strand at 4888.739 kb on + strand at 4888.739 kb on + strand at 4888.739 kb on + strand at 4888.739 kb on + strand at 4888.776 kb on - strand, within AO356_21835 at 4889.043 kb on - strand, within AO356_21835 at 4889.092 kb on - strand at 4889.119 kb on - strand at 4889.165 kb on + strand at 4889.310 kb on + strand at 4889.311 kb on - strand at 4889.311 kb on - strand at 4889.311 kb on - strand at 4889.314 kb on + strand at 4889.315 kb on - strand at 4889.326 kb on + strand at 4889.327 kb on - strand at 4889.327 kb on - strand at 4889.329 kb on + strand at 4889.330 kb on - strand at 4889.460 kb on - strand at 4889.460 kb on - strand at 4889.470 kb on - strand at 4889.545 kb on - strand, within AO356_21840 at 4889.604 kb on + strand, within AO356_21840 at 4889.649 kb on + strand, within AO356_21840 at 4889.649 kb on + strand, within AO356_21840 at 4889.649 kb on + strand, within AO356_21840 at 4889.649 kb on + strand, within AO356_21840 at 4889.649 kb on + strand, within AO356_21840 at 4889.649 kb on + strand, within AO356_21840 at 4889.650 kb on - strand, within AO356_21840 at 4889.650 kb on - strand, within AO356_21840 at 4889.751 kb on - strand, within AO356_21840 at 4890.232 kb on - strand, within AO356_21840 at 4890.250 kb on - strand, within AO356_21840 at 4890.250 kb on - strand, within AO356_21840 at 4890.250 kb on - strand, within AO356_21840 at 4890.267 kb on + strand, within AO356_21840 at 4890.267 kb on + strand, within AO356_21840 at 4890.267 kb on + strand, within AO356_21840 at 4890.267 kb on + strand, within AO356_21840 at 4890.267 kb on + strand, within AO356_21840 at 4890.268 kb on - strand, within AO356_21840 at 4890.268 kb on - strand, within AO356_21840 at 4890.268 kb on - strand, within AO356_21840 at 4890.268 kb on - strand, within AO356_21840 at 4890.750 kb on + strand, within AO356_21840 at 4890.867 kb on + strand, within AO356_21840 at 4890.868 kb on - strand, within AO356_21840 at 4891.087 kb on - strand, within AO356_21840 at 4891.087 kb on - strand, within AO356_21840 at 4891.188 kb on + strand at 4891.189 kb on - strand at 4891.285 kb on + strand at 4891.285 kb on + strand at 4891.286 kb on - strand at 4891.287 kb on + strand at 4891.287 kb on + strand at 4891.287 kb on + strand at 4891.287 kb on + strand at 4891.440 kb on + strand at 4891.441 kb on - strand at 4891.441 kb on - strand at 4891.527 kb on + strand at 4891.838 kb on - strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4892.093 kb on - strand, within AO356_21845 at 4892.189 kb on + strand, within AO356_21845 at 4892.190 kb on - strand, within AO356_21845 at 4892.192 kb on + strand, within AO356_21845 at 4892.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,888,370 + +1.2 4,888,370 + +1.9 4,888,616 + +1.5 4,888,616 + +0.8 4,888,722 - +0.2 4,888,722 - +0.1 4,888,739 + +0.3 4,888,739 + -1.6 4,888,739 + +0.2 4,888,739 + +2.6 4,888,776 - AO356_21835 0.15 +0.3 4,889,043 - AO356_21835 0.84 +2.0 4,889,092 - +0.5 4,889,119 - +1.3 4,889,165 + -2.7 4,889,310 + -2.7 4,889,311 - +0.8 4,889,311 - +1.5 4,889,311 - -0.5 4,889,314 + +1.0 4,889,315 - +1.2 4,889,326 + +0.2 4,889,327 - +0.2 4,889,327 - +0.6 4,889,329 + -1.4 4,889,330 - +1.4 4,889,460 - +0.5 4,889,460 - +0.2 4,889,470 - +0.8 4,889,545 - AO356_21840 0.13 -0.7 4,889,604 + AO356_21840 0.16 +1.3 4,889,649 + AO356_21840 0.18 -0.4 4,889,649 + AO356_21840 0.18 -0.5 4,889,649 + AO356_21840 0.18 +0.1 4,889,649 + AO356_21840 0.18 +0.5 4,889,649 + AO356_21840 0.18 +0.9 4,889,649 + AO356_21840 0.18 -2.4 4,889,650 - AO356_21840 0.18 +0.5 4,889,650 - AO356_21840 0.18 +3.8 4,889,751 - AO356_21840 0.23 +0.2 4,890,232 - AO356_21840 0.47 -0.5 4,890,250 - AO356_21840 0.48 +0.7 4,890,250 - AO356_21840 0.48 -0.4 4,890,250 - AO356_21840 0.48 -0.7 4,890,267 + AO356_21840 0.49 -0.5 4,890,267 + AO356_21840 0.49 +0.1 4,890,267 + AO356_21840 0.49 +2.3 4,890,267 + AO356_21840 0.49 +0.2 4,890,267 + AO356_21840 0.49 +0.9 4,890,268 - AO356_21840 0.49 +1.1 4,890,268 - AO356_21840 0.49 +0.8 4,890,268 - AO356_21840 0.49 +0.5 4,890,268 - AO356_21840 0.49 +0.9 4,890,750 + AO356_21840 0.72 -0.2 4,890,867 + AO356_21840 0.78 -1.3 4,890,868 - AO356_21840 0.78 +2.2 4,891,087 - AO356_21840 0.89 +1.7 4,891,087 - AO356_21840 0.89 -2.8 4,891,188 + -0.7 4,891,189 - +0.8 4,891,285 + +0.7 4,891,285 + -0.8 4,891,286 - +0.3 4,891,287 + +1.5 4,891,287 + +1.1 4,891,287 + +0.5 4,891,287 + -1.9 4,891,440 + +1.6 4,891,441 - +0.2 4,891,441 - -2.4 4,891,527 + -1.9 4,891,838 - AO356_21845 0.40 +1.9 4,891,963 + AO356_21845 0.56 -0.0 4,891,963 + AO356_21845 0.56 +1.7 4,891,963 + AO356_21845 0.56 -3.5 4,891,963 + AO356_21845 0.56 +1.5 4,891,963 + AO356_21845 0.56 +1.4 4,891,963 + AO356_21845 0.56 +0.7 4,891,963 + AO356_21845 0.56 -0.5 4,891,964 - AO356_21845 0.57 +1.5 4,891,964 - AO356_21845 0.57 +0.5 4,891,964 - AO356_21845 0.57 +1.0 4,891,964 - AO356_21845 0.57 +1.9 4,891,964 - AO356_21845 0.57 -0.7 4,892,093 - AO356_21845 0.74 +0.3 4,892,189 + AO356_21845 0.87 +0.2 4,892,190 - AO356_21845 0.87 +0.3 4,892,192 + AO356_21845 0.87 -0.3 4,892,260 - +1.3
Or see this region's nucleotide sequence