Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_20050 and AO356_20055 are separated by 193 nucleotides AO356_20055 and AO356_20060 are separated by 146 nucleotides AO356_20060 and AO356_20065 are separated by 65 nucleotides AO356_20065 and AO356_20070 overlap by 4 nucleotides
AO356_20050: AO356_20050 - hypothetical protein, at 4,487,986 to 4,488,765
_20050
AO356_20055: AO356_20055 - ATP-binding protein, at 4,488,959 to 4,489,474
_20055
AO356_20060: AO356_20060 - competence protein ComEC, at 4,489,621 to 4,491,867
_20060
AO356_20065: AO356_20065 - biopolymer transporter ExbB, at 4,491,933 to 4,492,598
_20065
AO356_20070: AO356_20070 - biopolymer transporter ExbD, at 4,492,595 to 4,493,023
_20070
Position (kb)
4489
4490
4491
4492 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4488.715 kb on + strand at 4488.715 kb on + strand at 4488.715 kb on + strand at 4488.715 kb on + strand at 4488.715 kb on + strand at 4488.716 kb on - strand at 4488.716 kb on - strand at 4488.716 kb on - strand at 4489.015 kb on - strand, within AO356_20055 at 4489.182 kb on + strand, within AO356_20055 at 4489.183 kb on - strand, within AO356_20055 at 4489.253 kb on - strand, within AO356_20055 at 4489.253 kb on - strand, within AO356_20055 at 4489.253 kb on - strand, within AO356_20055 at 4489.253 kb on - strand, within AO356_20055 at 4489.409 kb on + strand, within AO356_20055 at 4489.417 kb on + strand, within AO356_20055 at 4489.417 kb on + strand, within AO356_20055 at 4489.417 kb on + strand, within AO356_20055 at 4489.417 kb on + strand, within AO356_20055 at 4489.417 kb on + strand, within AO356_20055 at 4489.417 kb on + strand, within AO356_20055 at 4489.418 kb on - strand, within AO356_20055 at 4489.418 kb on - strand, within AO356_20055 at 4489.418 kb on - strand, within AO356_20055 at 4489.418 kb on - strand, within AO356_20055 at 4489.418 kb on - strand, within AO356_20055 at 4489.418 kb on - strand, within AO356_20055 at 4489.418 kb on - strand, within AO356_20055 at 4489.418 kb on - strand, within AO356_20055 at 4489.418 kb on - strand, within AO356_20055 at 4489.449 kb on + strand at 4489.449 kb on + strand at 4489.449 kb on + strand at 4489.449 kb on + strand at 4489.449 kb on + strand at 4489.449 kb on + strand at 4489.449 kb on + strand at 4489.449 kb on + strand at 4489.449 kb on + strand at 4489.450 kb on - strand at 4489.450 kb on - strand at 4489.450 kb on - strand at 4489.460 kb on + strand at 4489.474 kb on + strand at 4489.474 kb on + strand at 4489.514 kb on + strand at 4489.514 kb on + strand at 4489.515 kb on - strand at 4489.515 kb on - strand at 4489.515 kb on - strand at 4489.515 kb on - strand at 4489.515 kb on - strand at 4489.515 kb on - strand at 4489.515 kb on - strand at 4489.527 kb on + strand at 4489.587 kb on + strand at 4489.588 kb on - strand at 4489.588 kb on - strand at 4489.588 kb on - strand at 4489.588 kb on - strand at 4489.609 kb on - strand at 4489.726 kb on - strand at 4489.726 kb on - strand at 4489.726 kb on - strand at 4490.059 kb on + strand, within AO356_20060 at 4490.060 kb on - strand, within AO356_20060 at 4490.285 kb on + strand, within AO356_20060 at 4490.286 kb on - strand, within AO356_20060 at 4490.332 kb on + strand, within AO356_20060 at 4490.332 kb on + strand, within AO356_20060 at 4490.332 kb on + strand, within AO356_20060 at 4490.332 kb on + strand, within AO356_20060 at 4490.332 kb on + strand, within AO356_20060 at 4490.333 kb on - strand, within AO356_20060 at 4490.333 kb on - strand, within AO356_20060 at 4490.333 kb on - strand, within AO356_20060 at 4490.333 kb on - strand, within AO356_20060 at 4490.333 kb on - strand, within AO356_20060 at 4490.333 kb on - strand, within AO356_20060 at 4490.333 kb on - strand, within AO356_20060 at 4490.930 kb on + strand, within AO356_20060 at 4491.166 kb on + strand, within AO356_20060 at 4491.166 kb on + strand, within AO356_20060 at 4491.215 kb on + strand, within AO356_20060 at 4491.216 kb on - strand, within AO356_20060 at 4491.460 kb on - strand, within AO356_20060 at 4491.742 kb on + strand at 4491.743 kb on - strand at 4491.743 kb on - strand at 4491.743 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,488,715 + -0.1 4,488,715 + -0.0 4,488,715 + -1.5 4,488,715 + +1.0 4,488,715 + -0.2 4,488,716 - -2.6 4,488,716 - +0.1 4,488,716 - -1.5 4,489,015 - AO356_20055 0.11 +1.0 4,489,182 + AO356_20055 0.43 -0.0 4,489,183 - AO356_20055 0.43 -1.0 4,489,253 - AO356_20055 0.57 +0.9 4,489,253 - AO356_20055 0.57 -0.4 4,489,253 - AO356_20055 0.57 +0.3 4,489,253 - AO356_20055 0.57 +0.2 4,489,409 + AO356_20055 0.87 +1.8 4,489,417 + AO356_20055 0.89 +3.3 4,489,417 + AO356_20055 0.89 -0.8 4,489,417 + AO356_20055 0.89 +1.1 4,489,417 + AO356_20055 0.89 +0.1 4,489,417 + AO356_20055 0.89 +1.7 4,489,417 + AO356_20055 0.89 +0.8 4,489,418 - AO356_20055 0.89 -0.6 4,489,418 - AO356_20055 0.89 -2.3 4,489,418 - AO356_20055 0.89 +1.6 4,489,418 - AO356_20055 0.89 -2.5 4,489,418 - AO356_20055 0.89 +0.4 4,489,418 - AO356_20055 0.89 -1.1 4,489,418 - AO356_20055 0.89 +0.1 4,489,418 - AO356_20055 0.89 -0.3 4,489,418 - AO356_20055 0.89 +0.8 4,489,449 + -0.4 4,489,449 + -0.6 4,489,449 + +0.6 4,489,449 + -0.9 4,489,449 + +0.5 4,489,449 + +0.7 4,489,449 + -1.7 4,489,449 + -0.4 4,489,449 + -2.0 4,489,450 - -1.7 4,489,450 - +0.2 4,489,450 - -2.0 4,489,460 + -0.2 4,489,474 + -0.7 4,489,474 + +1.2 4,489,514 + -0.0 4,489,514 + -0.3 4,489,515 - +0.4 4,489,515 - -1.4 4,489,515 - -0.1 4,489,515 - +0.9 4,489,515 - -0.2 4,489,515 - +0.0 4,489,515 - -0.4 4,489,527 + -0.4 4,489,587 + -1.1 4,489,588 - +0.5 4,489,588 - +0.4 4,489,588 - -0.1 4,489,588 - -0.1 4,489,609 - -1.2 4,489,726 - +2.0 4,489,726 - -0.7 4,489,726 - +1.0 4,490,059 + AO356_20060 0.19 -1.4 4,490,060 - AO356_20060 0.20 +0.2 4,490,285 + AO356_20060 0.30 -1.9 4,490,286 - AO356_20060 0.30 -1.4 4,490,332 + AO356_20060 0.32 +2.1 4,490,332 + AO356_20060 0.32 -1.3 4,490,332 + AO356_20060 0.32 -0.0 4,490,332 + AO356_20060 0.32 -2.8 4,490,332 + AO356_20060 0.32 +2.2 4,490,333 - AO356_20060 0.32 +0.6 4,490,333 - AO356_20060 0.32 -3.2 4,490,333 - AO356_20060 0.32 -2.5 4,490,333 - AO356_20060 0.32 +0.6 4,490,333 - AO356_20060 0.32 -0.0 4,490,333 - AO356_20060 0.32 +0.2 4,490,333 - AO356_20060 0.32 +0.6 4,490,930 + AO356_20060 0.58 -1.9 4,491,166 + AO356_20060 0.69 -0.7 4,491,166 + AO356_20060 0.69 -0.1 4,491,215 + AO356_20060 0.71 +0.2 4,491,216 - AO356_20060 0.71 -1.3 4,491,460 - AO356_20060 0.82 +0.8 4,491,742 + +1.9 4,491,743 - -1.8 4,491,743 - +0.5 4,491,743 - +0.3
Or see this region's nucleotide sequence