Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_03275 and AO356_03280 are separated by 233 nucleotides AO356_03280 and AO356_03285 are separated by 105 nucleotides AO356_03285 and AO356_03290 are separated by 207 nucleotides
AO356_03275: AO356_03275 - acyl-CoA thioesterase, at 726,110 to 726,907
_03275
AO356_03280: AO356_03280 - metal-chelation protein CHAD, at 727,141 to 727,902
_03280
AO356_03285: AO356_03285 - hypothetical protein, at 728,008 to 728,298
_03285
AO356_03290: AO356_03290 - alpha/beta hydrolase, at 728,506 to 729,543
_03290
Position (kb)
727
728 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 726.225 kb on + strand, within AO356_03275 at 726.225 kb on + strand, within AO356_03275 at 726.225 kb on + strand, within AO356_03275 at 726.225 kb on + strand, within AO356_03275 at 726.226 kb on - strand, within AO356_03275 at 726.226 kb on - strand, within AO356_03275 at 726.433 kb on + strand, within AO356_03275 at 726.434 kb on - strand, within AO356_03275 at 726.513 kb on + strand, within AO356_03275 at 726.513 kb on + strand, within AO356_03275 at 726.514 kb on - strand, within AO356_03275 at 726.736 kb on + strand, within AO356_03275 at 726.737 kb on - strand, within AO356_03275 at 726.737 kb on - strand, within AO356_03275 at 726.784 kb on + strand, within AO356_03275 at 726.784 kb on + strand, within AO356_03275 at 726.785 kb on - strand, within AO356_03275 at 726.785 kb on - strand, within AO356_03275 at 726.792 kb on + strand, within AO356_03275 at 726.792 kb on + strand, within AO356_03275 at 726.792 kb on + strand, within AO356_03275 at 726.793 kb on - strand, within AO356_03275 at 726.793 kb on - strand, within AO356_03275 at 727.064 kb on + strand at 727.064 kb on + strand at 727.064 kb on + strand at 727.064 kb on + strand at 727.065 kb on - strand at 727.065 kb on - strand at 727.079 kb on - strand at 727.079 kb on - strand at 727.079 kb on - strand at 727.131 kb on + strand at 727.131 kb on + strand at 727.131 kb on + strand at 727.131 kb on + strand at 727.131 kb on + strand at 727.132 kb on - strand at 727.365 kb on - strand, within AO356_03280 at 727.365 kb on - strand, within AO356_03280 at 727.615 kb on + strand, within AO356_03280 at 727.615 kb on + strand, within AO356_03280 at 727.615 kb on + strand, within AO356_03280 at 727.616 kb on - strand, within AO356_03280 at 727.616 kb on - strand, within AO356_03280 at 727.616 kb on - strand, within AO356_03280 at 727.661 kb on + strand, within AO356_03280 at 727.661 kb on + strand, within AO356_03280 at 727.661 kb on + strand, within AO356_03280 at 727.661 kb on + strand, within AO356_03280 at 727.662 kb on - strand, within AO356_03280 at 727.662 kb on - strand, within AO356_03280 at 727.662 kb on - strand, within AO356_03280 at 727.908 kb on + strand at 727.908 kb on + strand at 727.908 kb on + strand at 727.908 kb on + strand at 727.908 kb on + strand at 727.908 kb on + strand at 727.909 kb on - strand at 727.909 kb on - strand at 727.909 kb on - strand at 727.909 kb on - strand at 727.909 kb on - strand at 727.909 kb on - strand at 727.915 kb on + strand at 727.916 kb on - strand at 727.944 kb on + strand at 727.945 kb on - strand at 727.945 kb on - strand at 727.978 kb on + strand at 727.978 kb on + strand at 727.978 kb on + strand at 727.979 kb on - strand at 727.979 kb on - strand at 728.036 kb on + strand at 728.036 kb on + strand at 728.036 kb on + strand at 728.132 kb on + strand, within AO356_03285 at 728.212 kb on - strand, within AO356_03285 at 728.302 kb on + strand at 728.335 kb on + strand at 728.335 kb on + strand at 728.335 kb on + strand at 728.335 kb on + strand at 728.398 kb on + strand at 728.398 kb on + strand at 728.528 kb on + strand at 728.529 kb on - strand at 728.554 kb on + strand at 728.562 kb on + strand at 728.596 kb on + strand at 728.650 kb on + strand, within AO356_03290 at 728.651 kb on - strand, within AO356_03290 at 728.651 kb on - strand, within AO356_03290 at 728.651 kb on - strand, within AO356_03290 at 728.701 kb on + strand, within AO356_03290 at 728.701 kb on + strand, within AO356_03290 at 728.701 kb on + strand, within AO356_03290 at 728.701 kb on + strand, within AO356_03290 at 728.702 kb on - strand, within AO356_03290 at 728.824 kb on + strand, within AO356_03290 at 728.824 kb on + strand, within AO356_03290 at 728.824 kb on + strand, within AO356_03290
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 726,225 + AO356_03275 0.14 +1.5 726,225 + AO356_03275 0.14 +1.1 726,225 + AO356_03275 0.14 +0.8 726,225 + AO356_03275 0.14 +1.3 726,226 - AO356_03275 0.15 -1.6 726,226 - AO356_03275 0.15 +1.3 726,433 + AO356_03275 0.40 +0.2 726,434 - AO356_03275 0.41 -3.0 726,513 + AO356_03275 0.51 +0.9 726,513 + AO356_03275 0.51 +0.2 726,514 - AO356_03275 0.51 +1.5 726,736 + AO356_03275 0.78 +1.0 726,737 - AO356_03275 0.79 -0.1 726,737 - AO356_03275 0.79 -2.2 726,784 + AO356_03275 0.84 -2.2 726,784 + AO356_03275 0.84 -1.0 726,785 - AO356_03275 0.85 +0.3 726,785 - AO356_03275 0.85 +0.3 726,792 + AO356_03275 0.85 -1.3 726,792 + AO356_03275 0.85 -0.6 726,792 + AO356_03275 0.85 -2.7 726,793 - AO356_03275 0.86 -1.0 726,793 - AO356_03275 0.86 +0.7 727,064 + +1.1 727,064 + +2.4 727,064 + -2.8 727,064 + +0.4 727,065 - +0.9 727,065 - -0.0 727,079 - -0.8 727,079 - +1.1 727,079 - +1.4 727,131 + +1.3 727,131 + +0.9 727,131 + -0.0 727,131 + +2.6 727,131 + -0.4 727,132 - +1.7 727,365 - AO356_03280 0.29 +1.0 727,365 - AO356_03280 0.29 -3.0 727,615 + AO356_03280 0.62 +1.8 727,615 + AO356_03280 0.62 -1.3 727,615 + AO356_03280 0.62 +0.9 727,616 - AO356_03280 0.62 -0.8 727,616 - AO356_03280 0.62 -0.6 727,616 - AO356_03280 0.62 +0.6 727,661 + AO356_03280 0.68 -1.5 727,661 + AO356_03280 0.68 +1.0 727,661 + AO356_03280 0.68 -0.8 727,661 + AO356_03280 0.68 +1.5 727,662 - AO356_03280 0.68 -0.4 727,662 - AO356_03280 0.68 -0.3 727,662 - AO356_03280 0.68 +0.7 727,908 + -0.5 727,908 + -1.1 727,908 + -0.0 727,908 + -2.1 727,908 + +0.1 727,908 + -0.5 727,909 - +1.1 727,909 - -0.8 727,909 - -0.3 727,909 - +0.1 727,909 - -2.5 727,909 - +3.8 727,915 + +0.7 727,916 - -1.4 727,944 + +0.1 727,945 - +0.3 727,945 - +2.2 727,978 + -0.6 727,978 + -1.0 727,978 + -0.9 727,979 - +1.0 727,979 - -1.2 728,036 + -1.1 728,036 + +1.9 728,036 + +2.0 728,132 + AO356_03285 0.43 +1.7 728,212 - AO356_03285 0.70 +0.4 728,302 + +2.0 728,335 + +0.6 728,335 + +0.7 728,335 + -2.7 728,335 + +1.1 728,398 + +1.5 728,398 + -2.9 728,528 + +0.3 728,529 - -0.4 728,554 + -0.2 728,562 + +0.8 728,596 + -0.0 728,650 + AO356_03290 0.14 -0.3 728,651 - AO356_03290 0.14 +0.4 728,651 - AO356_03290 0.14 +0.1 728,651 - AO356_03290 0.14 +0.9 728,701 + AO356_03290 0.19 +0.6 728,701 + AO356_03290 0.19 +1.1 728,701 + AO356_03290 0.19 -0.5 728,701 + AO356_03290 0.19 -1.8 728,702 - AO356_03290 0.19 -1.2 728,824 + AO356_03290 0.31 +0.6 728,824 + AO356_03290 0.31 +2.1 728,824 + AO356_03290 0.31 -2.9
Or see this region's nucleotide sequence