Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_03125 and AO356_03130 are separated by 64 nucleotides AO356_03130 and AO356_03135 are separated by 61 nucleotides AO356_03135 and AO356_03140 overlap by 11 nucleotides AO356_03140 and AO356_03145 are separated by 147 nucleotides
AO356_03125: AO356_03125 - cytoplasmic protein, at 699,442 to 700,671
_03125
AO356_03130: AO356_03130 - hypothetical protein, at 700,736 to 700,954
_03130
AO356_03135: AO356_03135 - LysR family transcriptional regulator, at 701,016 to 701,906
_03135
AO356_03140: AO356_03140 - hypothetical protein, at 701,896 to 702,645
_03140
AO356_03145: AO356_03145 - MarR family transcriptional regulator, at 702,793 to 703,254
_03145
Position (kb)
701
702 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 700.147 kb on + strand, within AO356_03125 at 700.313 kb on + strand, within AO356_03125 at 700.313 kb on + strand, within AO356_03125 at 700.314 kb on - strand, within AO356_03125 at 700.314 kb on - strand, within AO356_03125 at 700.314 kb on - strand, within AO356_03125 at 700.365 kb on - strand, within AO356_03125 at 700.365 kb on - strand, within AO356_03125 at 700.365 kb on - strand, within AO356_03125 at 700.365 kb on - strand, within AO356_03125 at 700.365 kb on - strand, within AO356_03125 at 700.496 kb on + strand, within AO356_03125 at 700.496 kb on + strand, within AO356_03125 at 700.496 kb on + strand, within AO356_03125 at 700.496 kb on + strand, within AO356_03125 at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.699 kb on - strand at 700.723 kb on - strand at 700.925 kb on + strand, within AO356_03130 at 700.926 kb on - strand, within AO356_03130 at 700.926 kb on - strand, within AO356_03130 at 700.931 kb on + strand, within AO356_03130 at 700.931 kb on + strand, within AO356_03130 at 700.931 kb on + strand, within AO356_03130 at 700.931 kb on + strand, within AO356_03130 at 700.931 kb on + strand, within AO356_03130 at 700.932 kb on - strand, within AO356_03130 at 700.932 kb on - strand, within AO356_03130 at 700.932 kb on - strand, within AO356_03130 at 700.932 kb on - strand, within AO356_03130 at 700.932 kb on - strand, within AO356_03130 at 700.932 kb on - strand, within AO356_03130 at 700.935 kb on + strand at 700.936 kb on - strand at 700.936 kb on - strand at 701.000 kb on - strand at 701.031 kb on + strand at 701.031 kb on + strand at 701.031 kb on + strand at 701.032 kb on - strand at 701.032 kb on - strand at 701.032 kb on - strand at 701.032 kb on - strand at 701.032 kb on - strand at 701.061 kb on + strand at 701.062 kb on - strand at 701.090 kb on + strand at 701.090 kb on + strand at 701.091 kb on - strand at 701.271 kb on + strand, within AO356_03135 at 701.330 kb on + strand, within AO356_03135 at 701.330 kb on + strand, within AO356_03135 at 701.331 kb on - strand, within AO356_03135 at 701.331 kb on - strand, within AO356_03135 at 701.331 kb on - strand, within AO356_03135 at 701.367 kb on + strand, within AO356_03135 at 701.368 kb on - strand, within AO356_03135 at 701.368 kb on - strand, within AO356_03135 at 701.368 kb on - strand, within AO356_03135 at 701.368 kb on - strand, within AO356_03135 at 701.368 kb on - strand, within AO356_03135 at 701.415 kb on + strand, within AO356_03135 at 701.682 kb on + strand, within AO356_03135 at 701.682 kb on + strand, within AO356_03135 at 701.683 kb on - strand, within AO356_03135 at 701.683 kb on - strand, within AO356_03135 at 701.779 kb on + strand, within AO356_03135 at 701.779 kb on + strand, within AO356_03135 at 702.069 kb on - strand, within AO356_03140 at 702.069 kb on - strand, within AO356_03140 at 702.069 kb on - strand, within AO356_03140 at 702.069 kb on - strand, within AO356_03140 at 702.069 kb on - strand, within AO356_03140 at 702.305 kb on + strand, within AO356_03140 at 702.305 kb on + strand, within AO356_03140 at 702.305 kb on + strand, within AO356_03140 at 702.305 kb on + strand, within AO356_03140 at 702.305 kb on + strand, within AO356_03140 at 702.306 kb on - strand, within AO356_03140 at 702.306 kb on - strand, within AO356_03140 at 702.306 kb on - strand, within AO356_03140 at 702.306 kb on - strand, within AO356_03140 at 702.306 kb on - strand, within AO356_03140 at 702.352 kb on - strand, within AO356_03140 at 702.352 kb on - strand, within AO356_03140 at 702.605 kb on - strand at 702.679 kb on + strand at 702.679 kb on + strand at 702.679 kb on + strand at 702.680 kb on - strand at 702.680 kb on - strand at 702.680 kb on - strand at 702.680 kb on - strand at 702.680 kb on - strand at 702.680 kb on - strand at 702.680 kb on - strand at 702.706 kb on + strand at 702.706 kb on + strand at 702.706 kb on + strand at 702.706 kb on + strand at 702.706 kb on + strand at 702.707 kb on - strand at 702.707 kb on - strand at 702.707 kb on - strand at 702.707 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 700,147 + AO356_03125 0.57 +0.2 700,313 + AO356_03125 0.71 +0.3 700,313 + AO356_03125 0.71 +1.7 700,314 - AO356_03125 0.71 -0.2 700,314 - AO356_03125 0.71 +2.6 700,314 - AO356_03125 0.71 -0.0 700,365 - AO356_03125 0.75 +0.5 700,365 - AO356_03125 0.75 +0.2 700,365 - AO356_03125 0.75 -0.0 700,365 - AO356_03125 0.75 +1.0 700,365 - AO356_03125 0.75 +0.8 700,496 + AO356_03125 0.86 +0.8 700,496 + AO356_03125 0.86 -0.6 700,496 + AO356_03125 0.86 -0.6 700,496 + AO356_03125 0.86 -0.1 700,698 + -2.6 700,698 + +1.3 700,698 + +0.2 700,698 + +1.7 700,698 + -1.4 700,698 + +1.5 700,698 + -1.0 700,698 + +1.3 700,698 + +1.6 700,698 + +1.1 700,699 - -0.4 700,723 - +0.6 700,925 + AO356_03130 0.86 +0.7 700,926 - AO356_03130 0.87 +1.3 700,926 - AO356_03130 0.87 +2.2 700,931 + AO356_03130 0.89 +0.5 700,931 + AO356_03130 0.89 -1.9 700,931 + AO356_03130 0.89 -0.4 700,931 + AO356_03130 0.89 +0.0 700,931 + AO356_03130 0.89 +1.0 700,932 - AO356_03130 0.89 +0.9 700,932 - AO356_03130 0.89 +0.7 700,932 - AO356_03130 0.89 +2.0 700,932 - AO356_03130 0.89 +0.6 700,932 - AO356_03130 0.89 +1.7 700,932 - AO356_03130 0.89 +1.2 700,935 + +1.6 700,936 - +0.5 700,936 - +0.4 701,000 - +0.5 701,031 + -1.3 701,031 + -2.0 701,031 + +0.7 701,032 - +0.3 701,032 - +1.2 701,032 - +0.8 701,032 - +2.0 701,032 - -1.3 701,061 + -1.0 701,062 - +0.7 701,090 + +1.8 701,090 + -1.7 701,091 - +1.8 701,271 + AO356_03135 0.29 -0.5 701,330 + AO356_03135 0.35 -0.7 701,330 + AO356_03135 0.35 -0.6 701,331 - AO356_03135 0.35 +2.3 701,331 - AO356_03135 0.35 -0.4 701,331 - AO356_03135 0.35 -0.0 701,367 + AO356_03135 0.39 +0.9 701,368 - AO356_03135 0.40 -0.8 701,368 - AO356_03135 0.40 +0.3 701,368 - AO356_03135 0.40 -0.3 701,368 - AO356_03135 0.40 +0.3 701,368 - AO356_03135 0.40 +1.0 701,415 + AO356_03135 0.45 -1.7 701,682 + AO356_03135 0.75 +0.4 701,682 + AO356_03135 0.75 +0.2 701,683 - AO356_03135 0.75 -1.7 701,683 - AO356_03135 0.75 -1.0 701,779 + AO356_03135 0.86 -2.4 701,779 + AO356_03135 0.86 +0.9 702,069 - AO356_03140 0.23 +0.5 702,069 - AO356_03140 0.23 -1.0 702,069 - AO356_03140 0.23 +0.7 702,069 - AO356_03140 0.23 +0.1 702,069 - AO356_03140 0.23 +1.5 702,305 + AO356_03140 0.55 -2.3 702,305 + AO356_03140 0.55 -1.1 702,305 + AO356_03140 0.55 -0.6 702,305 + AO356_03140 0.55 +2.3 702,305 + AO356_03140 0.55 -0.4 702,306 - AO356_03140 0.55 -2.0 702,306 - AO356_03140 0.55 +0.6 702,306 - AO356_03140 0.55 +0.0 702,306 - AO356_03140 0.55 +0.6 702,306 - AO356_03140 0.55 -0.9 702,352 - AO356_03140 0.61 +0.9 702,352 - AO356_03140 0.61 +1.0 702,605 - +0.8 702,679 + +0.5 702,679 + +0.5 702,679 + -0.9 702,680 - +0.8 702,680 - -0.6 702,680 - -0.5 702,680 - +1.7 702,680 - +0.6 702,680 - -1.3 702,680 - +2.2 702,706 + -0.4 702,706 + -0.3 702,706 + -2.8 702,706 + +0.2 702,706 + -0.7 702,707 - -1.2 702,707 - -0.2 702,707 - +2.5 702,707 - +0.5
Or see this region's nucleotide sequence