Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02735 and AO356_02740 overlap by 4 nucleotides AO356_02740 and AO356_02745 are separated by 4 nucleotides AO356_02745 and AO356_02750 are separated by 245 nucleotides
AO356_02735: AO356_02735 - type III secretion regulator InvE, at 599,242 to 600,348
_02735
AO356_02740: AO356_02740 - protein InvG, at 600,345 to 602,000
_02740
AO356_02745: AO356_02745 - transcriptional regulator, at 602,005 to 602,742
_02745
AO356_02750: AO356_02750 - nitrate transporter, at 602,988 to 604,199
_02750
Position (kb) 600
601
602
603 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 599.726 kb on + strand, within AO356_02735 at 599.727 kb on - strand, within AO356_02735 at 599.747 kb on + strand, within AO356_02735 at 599.790 kb on - strand, within AO356_02735 at 600.368 kb on - strand at 600.508 kb on + strand at 600.509 kb on - strand at 600.784 kb on + strand, within AO356_02740 at 600.832 kb on + strand, within AO356_02740 at 600.832 kb on + strand, within AO356_02740 at 600.833 kb on - strand, within AO356_02740 at 600.833 kb on - strand, within AO356_02740 at 600.918 kb on - strand, within AO356_02740 at 601.426 kb on + strand, within AO356_02740 at 601.546 kb on + strand, within AO356_02740 at 601.546 kb on + strand, within AO356_02740 at 601.546 kb on + strand, within AO356_02740 at 601.702 kb on + strand, within AO356_02740 at 601.702 kb on + strand, within AO356_02740 at 601.702 kb on + strand, within AO356_02740 at 601.702 kb on + strand, within AO356_02740 at 601.703 kb on - strand, within AO356_02740 at 601.703 kb on - strand, within AO356_02740 at 601.708 kb on + strand, within AO356_02740 at 601.907 kb on + strand at 601.907 kb on + strand at 601.908 kb on - strand at 601.909 kb on + strand at 601.909 kb on + strand at 601.910 kb on - strand at 601.910 kb on - strand at 602.021 kb on + strand at 602.022 kb on - strand at 602.152 kb on + strand, within AO356_02745 at 602.290 kb on - strand, within AO356_02745 at 602.360 kb on + strand, within AO356_02745 at 602.393 kb on + strand, within AO356_02745 at 602.393 kb on + strand, within AO356_02745 at 602.393 kb on + strand, within AO356_02745 at 602.394 kb on - strand, within AO356_02745 at 602.394 kb on - strand, within AO356_02745 at 602.503 kb on - strand, within AO356_02745 at 602.839 kb on + strand at 602.839 kb on + strand at 602.839 kb on + strand at 602.855 kb on - strand at 602.967 kb on + strand at 602.968 kb on - strand at 602.968 kb on - strand at 602.973 kb on + strand at 602.974 kb on - strand at 602.974 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 599,726 + AO356_02735 0.44 +3.1 599,727 - AO356_02735 0.44 -0.2 599,747 + AO356_02735 0.46 +0.3 599,790 - AO356_02735 0.50 +1.4 600,368 - +0.5 600,508 + +0.0 600,509 - +0.9 600,784 + AO356_02740 0.27 +0.6 600,832 + AO356_02740 0.29 -0.4 600,832 + AO356_02740 0.29 +1.7 600,833 - AO356_02740 0.29 -0.2 600,833 - AO356_02740 0.29 -0.4 600,918 - AO356_02740 0.35 +0.1 601,426 + AO356_02740 0.65 -0.9 601,546 + AO356_02740 0.73 +0.9 601,546 + AO356_02740 0.73 +0.8 601,546 + AO356_02740 0.73 +0.8 601,702 + AO356_02740 0.82 +1.9 601,702 + AO356_02740 0.82 -1.5 601,702 + AO356_02740 0.82 -0.3 601,702 + AO356_02740 0.82 +0.5 601,703 - AO356_02740 0.82 -1.0 601,703 - AO356_02740 0.82 +0.2 601,708 + AO356_02740 0.82 +0.5 601,907 + +0.4 601,907 + -1.1 601,908 - +0.0 601,909 + -1.1 601,909 + -0.7 601,910 - -1.0 601,910 - +0.5 602,021 + -1.0 602,022 - +0.4 602,152 + AO356_02745 0.20 -0.3 602,290 - AO356_02745 0.39 -2.4 602,360 + AO356_02745 0.48 -0.1 602,393 + AO356_02745 0.53 +0.8 602,393 + AO356_02745 0.53 +0.8 602,393 + AO356_02745 0.53 +0.2 602,394 - AO356_02745 0.53 -3.3 602,394 - AO356_02745 0.53 +2.6 602,503 - AO356_02745 0.67 -1.3 602,839 + +0.6 602,839 + +0.4 602,839 + -2.1 602,855 - -2.2 602,967 + -0.9 602,968 - -2.2 602,968 - +0.4 602,973 + +0.1 602,974 - +0.3 602,974 - -0.4
Or see this region's nucleotide sequence