Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_00970 and AO356_00975 are separated by 133 nucleotides AO356_00975 and AO356_00980 are separated by 204 nucleotides AO356_00980 and AO356_00985 are separated by 5 nucleotides AO356_00985 and AO356_00990 are separated by 156 nucleotides
AO356_00970: AO356_00970 - LacI family transcriptional regulator, at 220,733 to 221,692
_00970
AO356_00975: AO356_00975 - hypothetical protein, at 221,826 to 222,278
_00975
AO356_00980: AO356_00980 - DNA damage resistance protein <small>(from data)</small>, at 222,483 to 222,782
_00980
AO356_00985: AO356_00985 - deoxyribonuclease, at 222,788 to 223,477
_00985
AO356_00990: AO356_00990 - penicillin-binding protein activator LpoB, at 223,634 to 223,999
_00990
Position (kb) 222
223 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 221.517 kb on + strand, within AO356_00970 at 221.518 kb on - strand, within AO356_00970 at 221.518 kb on - strand, within AO356_00970 at 221.624 kb on - strand at 221.709 kb on + strand at 221.709 kb on + strand at 221.710 kb on - strand at 221.715 kb on + strand at 221.715 kb on + strand at 221.716 kb on - strand at 221.770 kb on + strand at 221.771 kb on - strand at 221.771 kb on - strand at 221.771 kb on - strand at 221.775 kb on + strand at 221.837 kb on + strand at 221.837 kb on + strand at 221.838 kb on - strand at 221.977 kb on + strand, within AO356_00975 at 222.161 kb on + strand, within AO356_00975 at 222.305 kb on + strand at 222.305 kb on + strand at 222.306 kb on - strand at 222.393 kb on + strand at 222.394 kb on - strand at 222.472 kb on + strand at 222.472 kb on + strand at 222.472 kb on + strand at 222.473 kb on - strand at 222.473 kb on - strand at 222.480 kb on + strand at 222.498 kb on + strand at 222.519 kb on - strand, within AO356_00980 at 222.528 kb on - strand, within AO356_00980 at 222.565 kb on + strand, within AO356_00980 at 222.566 kb on - strand, within AO356_00980 at 222.566 kb on - strand, within AO356_00980 at 222.566 kb on - strand, within AO356_00980 at 222.574 kb on - strand, within AO356_00980 at 222.574 kb on - strand, within AO356_00980 at 222.574 kb on - strand, within AO356_00980 at 222.575 kb on + strand, within AO356_00980 at 222.575 kb on + strand, within AO356_00980 at 222.576 kb on - strand, within AO356_00980 at 222.805 kb on - strand at 222.893 kb on + strand, within AO356_00985 at 222.893 kb on + strand, within AO356_00985 at 222.893 kb on + strand, within AO356_00985 at 222.893 kb on + strand, within AO356_00985 at 222.894 kb on - strand, within AO356_00985 at 222.894 kb on - strand, within AO356_00985 at 222.894 kb on - strand, within AO356_00985 at 222.894 kb on - strand, within AO356_00985 at 222.917 kb on + strand, within AO356_00985 at 222.918 kb on - strand, within AO356_00985 at 222.928 kb on + strand, within AO356_00985 at 222.928 kb on + strand, within AO356_00985 at 222.929 kb on - strand, within AO356_00985 at 222.932 kb on + strand, within AO356_00985 at 222.933 kb on - strand, within AO356_00985 at 222.972 kb on + strand, within AO356_00985 at 222.972 kb on + strand, within AO356_00985 at 223.018 kb on + strand, within AO356_00985 at 223.018 kb on + strand, within AO356_00985 at 223.018 kb on + strand, within AO356_00985 at 223.018 kb on + strand, within AO356_00985 at 223.018 kb on + strand, within AO356_00985 at 223.019 kb on - strand, within AO356_00985 at 223.019 kb on - strand, within AO356_00985 at 223.019 kb on - strand, within AO356_00985 at 223.103 kb on + strand, within AO356_00985 at 223.103 kb on + strand, within AO356_00985 at 223.104 kb on - strand, within AO356_00985 at 223.104 kb on - strand, within AO356_00985 at 223.295 kb on + strand, within AO356_00985 at 223.295 kb on + strand, within AO356_00985 at 223.295 kb on + strand, within AO356_00985 at 223.301 kb on + strand, within AO356_00985 at 223.302 kb on - strand, within AO356_00985 at 223.317 kb on - strand, within AO356_00985 at 223.352 kb on + strand, within AO356_00985 at 223.352 kb on + strand, within AO356_00985 at 223.352 kb on + strand, within AO356_00985 at 223.352 kb on + strand, within AO356_00985 at 223.352 kb on + strand, within AO356_00985 at 223.373 kb on + strand, within AO356_00985 at 223.635 kb on + strand at 223.636 kb on - strand at 223.651 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 221,517 + AO356_00970 0.82 -0.6 221,518 - AO356_00970 0.82 +0.5 221,518 - AO356_00970 0.82 -1.4 221,624 - +0.2 221,709 + +0.4 221,709 + -0.2 221,710 - +1.7 221,715 + +0.5 221,715 + -0.8 221,716 - -0.2 221,770 + -0.2 221,771 - +1.6 221,771 - -1.3 221,771 - -1.9 221,775 + -0.4 221,837 + +2.1 221,837 + -1.3 221,838 - +1.2 221,977 + AO356_00975 0.33 -0.8 222,161 + AO356_00975 0.74 -0.3 222,305 + -1.0 222,305 + -0.2 222,306 - +0.4 222,393 + +0.7 222,394 - +0.1 222,472 + +0.1 222,472 + -0.4 222,472 + +1.8 222,473 - +1.1 222,473 - -0.2 222,480 + +3.2 222,498 + +0.6 222,519 - AO356_00980 0.12 +0.6 222,528 - AO356_00980 0.15 -1.1 222,565 + AO356_00980 0.27 -1.3 222,566 - AO356_00980 0.28 -2.6 222,566 - AO356_00980 0.28 -1.5 222,566 - AO356_00980 0.28 -2.1 222,574 - AO356_00980 0.30 -1.4 222,574 - AO356_00980 0.30 +2.0 222,574 - AO356_00980 0.30 -1.9 222,575 + AO356_00980 0.31 -2.2 222,575 + AO356_00980 0.31 +0.1 222,576 - AO356_00980 0.31 -0.3 222,805 - +0.3 222,893 + AO356_00985 0.15 +0.9 222,893 + AO356_00985 0.15 +2.4 222,893 + AO356_00985 0.15 +0.4 222,893 + AO356_00985 0.15 +0.9 222,894 - AO356_00985 0.15 +1.8 222,894 - AO356_00985 0.15 -0.2 222,894 - AO356_00985 0.15 +1.7 222,894 - AO356_00985 0.15 -0.1 222,917 + AO356_00985 0.19 +2.1 222,918 - AO356_00985 0.19 -0.3 222,928 + AO356_00985 0.20 +0.5 222,928 + AO356_00985 0.20 -0.2 222,929 - AO356_00985 0.20 -2.1 222,932 + AO356_00985 0.21 +1.8 222,933 - AO356_00985 0.21 -0.0 222,972 + AO356_00985 0.27 +2.0 222,972 + AO356_00985 0.27 -0.1 223,018 + AO356_00985 0.33 -0.4 223,018 + AO356_00985 0.33 +1.2 223,018 + AO356_00985 0.33 +1.4 223,018 + AO356_00985 0.33 -0.2 223,018 + AO356_00985 0.33 -0.7 223,019 - AO356_00985 0.33 -0.3 223,019 - AO356_00985 0.33 -0.0 223,019 - AO356_00985 0.33 +0.3 223,103 + AO356_00985 0.46 +1.6 223,103 + AO356_00985 0.46 -0.8 223,104 - AO356_00985 0.46 +0.1 223,104 - AO356_00985 0.46 -2.1 223,295 + AO356_00985 0.73 +0.1 223,295 + AO356_00985 0.73 +1.0 223,295 + AO356_00985 0.73 +1.5 223,301 + AO356_00985 0.74 -0.9 223,302 - AO356_00985 0.74 -1.4 223,317 - AO356_00985 0.77 +1.3 223,352 + AO356_00985 0.82 -0.7 223,352 + AO356_00985 0.82 -0.1 223,352 + AO356_00985 0.82 +0.3 223,352 + AO356_00985 0.82 -0.1 223,352 + AO356_00985 0.82 -1.6 223,373 + AO356_00985 0.85 -0.0 223,635 + -1.6 223,636 - -0.1 223,651 - -2.6
Or see this region's nucleotide sequence