Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_00780 and AO356_00785 overlap by 4 nucleotides AO356_00785 and AO356_00790 are separated by 188 nucleotides AO356_00790 and AO356_00795 overlap by 11 nucleotides
AO356_00780: AO356_00780 - methyltransferase, at 177,005 to 177,751
_00780
AO356_00785: AO356_00785 - polysaccharide synthesis protein GtrA, at 177,748 to 178,155
_00785
AO356_00790: AO356_00790 - epimerase, at 178,344 to 179,441
_00790
AO356_00795: AO356_00795 - 4Fe-4S ferredoxin, at 179,431 to 181,017
_00795
Position (kb) 178
179
180 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 177.417 kb on - strand, within AO356_00780 at 177.515 kb on + strand, within AO356_00780 at 177.516 kb on - strand, within AO356_00780 at 177.600 kb on - strand, within AO356_00780 at 177.642 kb on + strand, within AO356_00780 at 177.787 kb on + strand at 177.788 kb on - strand at 177.828 kb on - strand, within AO356_00785 at 177.956 kb on - strand, within AO356_00785 at 177.956 kb on - strand, within AO356_00785 at 177.956 kb on - strand, within AO356_00785 at 178.100 kb on + strand, within AO356_00785 at 178.100 kb on + strand, within AO356_00785 at 178.165 kb on + strand at 178.165 kb on + strand at 178.165 kb on + strand at 178.166 kb on - strand at 178.166 kb on - strand at 178.166 kb on - strand at 178.188 kb on + strand at 178.189 kb on - strand at 178.189 kb on - strand at 178.243 kb on - strand at 178.287 kb on + strand at 178.287 kb on + strand at 178.288 kb on - strand at 178.491 kb on + strand, within AO356_00790 at 178.492 kb on - strand, within AO356_00790 at 178.492 kb on - strand, within AO356_00790 at 178.547 kb on - strand, within AO356_00790 at 178.547 kb on - strand, within AO356_00790 at 179.052 kb on + strand, within AO356_00790 at 179.924 kb on - strand, within AO356_00795 at 180.140 kb on - strand, within AO356_00795 at 180.175 kb on + strand, within AO356_00795
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 177,417 - AO356_00780 0.55 -3.8 177,515 + AO356_00780 0.68 +0.2 177,516 - AO356_00780 0.68 +0.4 177,600 - AO356_00780 0.80 -1.3 177,642 + AO356_00780 0.85 +2.7 177,787 + -0.9 177,788 - -1.1 177,828 - AO356_00785 0.20 -0.8 177,956 - AO356_00785 0.51 -1.3 177,956 - AO356_00785 0.51 +0.4 177,956 - AO356_00785 0.51 +1.0 178,100 + AO356_00785 0.86 -1.4 178,100 + AO356_00785 0.86 +0.2 178,165 + -0.7 178,165 + +0.5 178,165 + -0.7 178,166 - -1.1 178,166 - -1.3 178,166 - -0.4 178,188 + +0.1 178,189 - +0.8 178,189 - -1.3 178,243 - +2.7 178,287 + +0.3 178,287 + -2.5 178,288 - +1.7 178,491 + AO356_00790 0.13 +1.4 178,492 - AO356_00790 0.13 -0.5 178,492 - AO356_00790 0.13 +1.0 178,547 - AO356_00790 0.18 +0.2 178,547 - AO356_00790 0.18 +1.1 179,052 + AO356_00790 0.64 +0.7 179,924 - AO356_00795 0.31 +0.6 180,140 - AO356_00795 0.45 +1.1 180,175 + AO356_00795 0.47 +1.3
Or see this region's nucleotide sequence